Proyecto Scial

En la actualidad, la pandemia originada por el Covid-19 ha afectado negativamente a muchos rubros de trabajo, generando una alta tasa de desempleo en el Perú, sobre todo a los pequeños y medianos negocios. No obstante, en este contexto el mundo se ha digitalizado y los servicios digitales han recibido un nuevo potencial. Un estudio realizado por Ipsos, en el 2021, reveló que las áreas más demandadas por emprendedores son las de marketing y publicidad con 43% y el manejo de herramientas digitales con un 26%. Sin embargo, aún existe un gran número de emprendedores que no logran adaptarse a las nuevas tendencias y continúan dirigiendo su negocio con poco éxito. Considerando ello, nuestro proyecto busca brindar una solución eficiente a través de la creación de una página web enfocada a las personas residentes de Lima Metropolitana con el objetivo de satisfacer la necesidad en el marketing digital y dirección estratégica e impulsar de manera eficiente la rentabilidad y mejorar el alcance de los emprendimientos a través de cursos y servicios online. Para la sustentación de dicho proyecto se desarrollaron diferentes experimentos que facultaron la validez de la necesidad hallada, las posibles soluciones planteadas y el resultado del concierge. Los resultados que se obtuvieron a partir de los experimentos realizados nos ayudaron a entender la importancia que tiene el validar el modelo de negocio; así como también validar el nivel de intención de compra del target. Se otorga a disposición del público el presente trabajo de investigación en beneficio del crecimiento de nuevos proyectos e ideas sostenibles que continúen con el camino de la constante innovación.At present, the pandemic caused by Covid-19 has negatively affected many areas of work, generating a high unemployment rate in Peru, especially for small and medium-sized businesses.  However, in this context, the world has gone digital and digital services have been given new potential. A study conducted by Ipsos in 2021 revealed that the areas most in demand by entrepreneurs are marketing and advertising with 43% and the management of digital tools with 26%. Nevertheless, there are still a large number of entrepreneurs who are unable to adapt to new trends and continue to run their businesses with little success. Considering this, our project seeks to provide an efficient solution through the creation of a website focused on the residents of Metropolitan Lima with the aim of satisfying the need in digital marketing and strategic management and efficiently boosting the profitability and improving the scope of enterprises through online courses and services.  In order to support the project, different experiments were carried out to validate the need found, the possible solutions proposed and the results of the concierge. The results obtained from the experiments helped us to understand the importance of validating the business model, as well as validating the level of purchase intention of the target. In view of the above, we make this investigation work available to the public for the benefit of the growth of new projects and sustainable ideas that continue on the path of constant innovation.Trabajo de investigació

Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)

In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field