Phenotypic and genotypic assay for detection of extended spectrum B-lactamases production by Klebsiella pnemoniae isolates in Emam Reza Hospital in Tabriz, Iran.

Objectives of this study were to investigate the prevalence of K. pneumoniae producing ESBLs, to evaluate the susceptibility of K. pneumoniae producing ESBLs towards non-beta-lactam antibiotics and to study the dominant ESBLs gene in Emam Reza hospital. K. pneumoniae producing ESBLs identified by phenotypic and genotypic methods. Polymerase Chain Reaction (PCR) performed for detection of blaSHV, TEM and CTX-M. The findings showed that 43.69%, 13.59%, 7.77%, 11.65% and 23.3% were from UTI, ICUs, surgery ward, lesion infections and RTI, respectively. The results showed that 43.7% of isolates were ESBLs produces. The findings revealed that 26.7%, 6.7%, 20% and 0% of K.pneumoniae producing ESBLs were resistant to amikacin, ciprofloxacin, cotrimoxazol and imipenem, respectively. Thirty-nine blaSHV, seven blaTEM and seven blaCTX-M identified among K.pneumoniae producing ESBLs. The results reflected in cold month resistant to third generation cephalosporins were more than warm months. Generally, frequency of blaSHV was more than blaCTX-M and blaTEM

Extended spectrum beta-lactamases among hospitalized patients in surgery wards, Ilam, Iran.

Objectives of this study were to study the molecular epidemiology of ESBL-producing Klebsiella pneumoniae in selective hospitals in Iran, to determine the prevalence of TEM, SHV and CTX-M genes responsible for ESBL production among ESBL-producing K. pneumoniae, to investigate the susceptibility of Klebsiellae spp producing ESBLs towards non-beta-lactam antibiotics, all in different seasons. Clinical isolates of K. pneumoniae were identified during Mar. 2007 to Apr. 2008 in Ilam hospitals, the province in west of Iran. All isolates were found in surgery wards. ESBL activity was first evaluated using the standard disc diffusion test for cephalosporins and monobactam, then using the double-disc synergy test between cephalosporins and clavulanate. PCR assay had done for ESBLs genes detection. The results showed sixteen K.pneumoniae were identified by chemical methods. No resistance had occurred among K.pneumoniae toward non-beta-lactam antibiotics. BlaSHV was dominant gene responsible for ESBLs production while just one blaTEM along with blaSHV were found. BlaCTX-M was not responsible for ESBLs production in our study. 37.5% K.pneumoniae producing ESBLs in surgery ward in west of Iran must be considerable and need to further study in different part of hospital in Ilam and in Iran. Strict antibiotic policy should be adopted in hospitals to estimate the impact of higher resistance in bacteria and to take steps for reducing these resistances