On the spectrum of atmospheric velocity fluctuations seen by MST/ST radar and their interpretation

The observations of the spectrum of atmospheric motions over the range of periods from a few minutes to many hours are considered that have been made with stratosphere-troposphere/mesosphere-stratosphere (ST/MST) radars in the past five years. This range of periods includes the periods associated with buoyancy waves and the scale of atmospheric motions often referred to by meteorologists as the mesoscale. The spectra of horizontal and vertical velocities are considered. Their interpretation is examined in terms of buoyancy wave theory and turbulence theory. To help in interpreting these spectra some recently determined aircraft wave number spectra are presented

Cereal Leaf Beetle (Coleoptera: Chrysomelidae) Influence of Seeding Rate of Oats on Populations

In field and greenhouse studies, more cereal leaf beetle [Oulema melanopus (Lin- naeus)] eggs and larvae were found per unit area on spring oats, Avena sativa L., planted either at intermediate (54 kg/ha) or high (136 kg/ha) seeding rates, than when planted at a lower seeding rate (14 kg/ha). However, there were fewer eggs and larvae per stem in plantings of the high or intermediate rates than in those of the lower rate. Oats should not be planted at less than the recommended rates in beetle-infested areas

Laparoscopic versus open colorectal resection for cancer and polyps: A cost-effectiveness study

Methods: Participants were recruited in 2006-2007 in a district general hospital in the south of England; those with a diagnosis of cancer or polyps were included in the analysis. Quality of life data were collected using EQ-5D, on alternate days after surgery for 4 weeks. Costs per patient, from a National Health Service perspective (in British pounds, 2006) comprised the sum of operative, hospital, and community costs. Missing data were filled using multiple imputation methods. The difference in mean quality adjusted life years and costs between surgery groups were estimated simultaneously using a multivariate regression model applied to 20 imputed datasets. The probability that laparoscopic surgery is cost-effective compared to open surgery for a given societal willingness-to-pay threshold is illustrated using a cost-effectiveness acceptability curve

Structural and Functional Studies of the KCNQ1-KCNE K\u3csup\u3e+\u3c/sup\u3e Channel Complex: A Dissertation

KCNQ1 is a homotetrameric voltage-gated potassium channel expressed in cardiomyocytes and epithelial tissues. However, currents arising from KCNQ1 have never been physiologically observed. KCNQ1 is able to provide the diverse potassium conductances required by these distinct cell types through coassembly with and modulation by type I transmembrane β-subunits of the KCNE gene family. KCNQ1-KCNE K+ channels play important physiological roles. In cardiac tissues the association of KCNQ1 with KCNE1 gives rise to IKs, the slow delayed outwardly rectifying potassium current. IKs is in part responsible for repolarizing heart muscle, and is therefore crucial in maintaining normal heart rhymicity. IKschannels help terminate each action potential and provide cardiac repolarization reserve. As such, mutations in either subunit can lead to Romano-Ward Syndrome or Jervell and Lange-Nielsen Syndrome, two forms of Q-T prolongation. In epithelial cells, KCNQ1-KCNE1, KCNQ1-KCNE2 and KCNQ1-KCNE3 give rise to potassium currents required for potassium recycling and secretion. These functions arise because the biophysical properties of KCNQ1 are always dramatically altered by KCNE co-expression. We wanted to understand how KCNE peptides are able to modulate KCNQ1. In Chapter II, we produce partial truncations of KCNE3 and demonstrate the transmembrane domain is necessary and sufficient for both assembly with and modulation of KCNQ1. Comparing these results with published results obtained from chimeric KCNE peptides and partial deletion mutants of KCNE1, we propose a bipartite modulation residing in KCNE peptides. Transmembrane modulation is either active (KCNE3) or permissive (KCNE1). Active transmembrane KCNE modulation masks juxtamembranous C-terminal modulation of KCNQ1, while permissive modulation allows C-terminal modulation of KCNQ1 to express. We test our hypothesis, and demonstrate C-terminal Long QT point mutants in KCNE1 can be masked by active trasnsmembrane modulation. Having confirmed the importance the C-terminus of KCNE1, we continue with two projects designed to elucidate KCNE1 C-terminal structure. In Chapter III we conduct an alanine-perturbation scan within the C-terminus. C-terminal KCNE1 alanine point mutations result in changes in the free energy for the KCNQ1-KCNE1 channel complex. High-impact point mutants cluster in an arrangement consistent with an alphahelical secondary structure, kinked by a single proline residue. In Chapter IV, we use oxidant-mediated disulfide bond formation between non-native cysteine residues to demonstrate amino acid side chains residing within the C-terminal domain of KCNE1 are close and juxtaposed to amino acid side chains on the cytoplasmic face of the KCNQ1 pore domain. Many of the amino acids identified as high impact through alanine perturbation correspond with residues identified as able to form disulfide bonds with KCNQ1. Taken together, we demonstrate that the interaction between the C-terminus of KCNE1 and the pore domain of KCNQ1 is required for the proper modulation of KCNQ1 by KCNE1, and by extension, normal IKs function and heart rhymicity

Ecological Observations on Predatory Coccinellidae (Coleoptera) in Southwestern Michigan

Ecological observations on habitat utilization by thirteen species of predatory Coccinellidae were made at a southern Michigan site during 1989 and 1990. Most of species were common during both years and used both agricul- tural and uncultivated habitats. Coccinella septempunctata and Coleomegilla maculata, were the most abundant in agricultural crops (alfalfa, maize, soy- bean and triticale), whereas Adalia bipunctata and Cycloneda munda, were the most abundant in deciduous and bushy habitats

The proposed flatland radar

A flexible very high frequency (VHF) stratosphere-troposphere (ST) radar configured for meteorological research is to be constructed near Urbana, Illinois. Measurement of small vertical velocities associated with synoptic-scale meteorology can be performed. A large Doppler microwave radar (CHILL) is located a few km from the site of the proposed ST radar. Since the microwave radar can measure the location and velocity of hydrometeors and the VHF ST radar can measure clear (or cloudy) air velocities, simultaneous observations by these two radars of stratiform or convective weather systems would provide valuable meteorological information

Application of Risk Informed Decision Making to Highly Reliable Three Dimensionally Woven Thermal Protection System for Mars Sample Return

The NASA Risk Informed Decision Making process is used to assess a trade space of three dimensionally woven thermal protection systems for application to the Mars Sample Return Earth Entry Vehicle. Candidate architectures are assessed based on mission assurance, technical development, cost, and schedule risk. Assessment methodology differed between the architectures, utilizing a four-point quantitative scale for mission assurance and technical development and highly tailored PERT techniques for cost and schedule. Risk results are presented, in addition to a review of RIDM effectiveness for this application

KCNE3 Truncation Mutants Reveal a Bipartite Modulation of KCNQ1 K+ Channels

The five KCNE genes encode a family of type I transmembrane peptides that assemble with KCNQ1 and other voltage-gated K+ channels, resulting in potassium conducting complexes with varied channel-gating properties. It has been recently proposed that a triplet of amino acids within the transmembrane domain of KCNE1 and KCNE3 confers modulation specificity to the peptide, since swapping of these three residues essentially converts the recipient KCNE into the donor (Melman, Y.F., A. Domenech, S. de la Luna, and T.V. McDonald. 2001. J. Biol. Chem. 276:6439–6444). However, these results are in stark contrast with earlier KCNE1 deletion studies, which demonstrated that a COOH-terminal region, highly conserved between KCNE1 and KCNE3, was responsible for KCNE1 modulation of KCNQ1 (Tapper, A.R., and A.L. George. 2000 J. Gen. Physiol. 116:379–389.). To ascertain whether KCNE3 peptides behave similarly to KCNE1, we examined a panel of NH2- and COOH-terminal KCNE3 truncation mutants to directly determine the regions required for assembly with and modulation of KCNQ1 channels. Truncations lacking the majority of their NH2 terminus, COOH terminus, or mutants harboring both truncations gave rise to KCNQ1 channel complexes with basal activation, a hallmark of KCNE3 modulation. These results demonstrate that the KCNE3 transmembrane domain is sufficient for assembly with and modulation of KCNQ1 channels and suggests a bipartite model for KCNQ1 modulation by KCNE1 and KCNE3 subunits. In this model, the KCNE3 transmembrane domain is active in modulation and overrides the COOH terminus' contribution, whereas the KCNE1 transmembrane domain is passive and reveals COOH-terminal modulation of KCNQ1 channels. We furthermore test the validity of this model by using the active KCNE3 transmembrane domain to functionally rescue a nonconducting, yet assembly and trafficking competent, long QT mutation located in the conserved COOH-terminal region of KCNE1