31 research outputs found
An Assessment of Desolvation on Rates of Acetyl Transfer: Insights into Enzyme Catalysis
Growth promoting and inhibitory activities of 3t3 and other cell lines for thiol-dependent lymphoma cells in vitro.
Production of autoinhibitory factors by mouse lymphoma cells in vitro and its relationship to thiol dependence.
Five different kinds of mouse lymphoma cells conditioned culture medium so that it became inhibitory to thiol-dependent cells but not to thiol-independent cells. Conditioning was rapid and appeared to be complete in 1-3 hr. The inhibitory effects could be reversed by concentrations of 2-mercaptoethanol higher than those usually required for growth promotion. Inhibitory factors were concentrated by ultrafiltration and chromatography on Sephadex G-10 which showed their apparent molecular weights to be 300-600. The factors were stable at 100 degrees and to proteases but were inactivated (after reduction) by iodoacetamide, indicating that they may be thiols or disulfides
Growth promoting and inhibitory activities of 3t3 and other cell lines for thiol-dependent lymphoma cells in vitro.
Проводится анализ становления и развития институтов невозможности исполнения обязательства в Турции, Швейцарии, Германии и России. Исследование позволяет заключить: в гражданском законодательстве Турции нашла закрепление и продолжает развиваться заимствованная из швейцарского гражданского права и критикуемая современной европейской цивилистикой пандектная модель рассматриваемого института, от которой в 2002 г. отказался немецкий законодатель и лишь отдельные элементы которой реализованы в ГК РФ
Production of autoinhibitory factors by mouse lymphoma cells in vitro and its relationship to thiol dependence.
Macromolecular inhibitory factor for lymphoid cells produced by mouse macrophages.
Supernatants of high density cultures of mouse peritoneal macrophages were inhibitory to homologous lymphoid cells in vitro by a number of parameters: proliferation and survival of lymphoma cells was impaired, the immune response of spleen cells to sheep red cells and to Concanavalin A (Con A) was decreased. Inhibition was due to factor(s) with mol. wt of approximately 110,000 as estimated by gel filtration. Activity was stable to heating at 56 degrees, and was resistant to trypsin but not to pronase