a) Analytical sensitivity of the PCR amplification of the Hexokinase (HK) gene (product size 197 bp).

<p>Lanes 1–8 were 10-fold dilutions of leishmanial genomic DNA from 10 ng to 1 fg per reaction. Lane 9 was the negative control and molecular sizes were compared with a 50 bp molecular weight ladder (MW). b) Analytical sensitivity of the PCR amplification of the Phosphoglucomutase (PGM) gene (product size 278 bp). Lane 1–8 were 10-fold dilutions of leishmanial genomic DNA from 1 ng to 1 fg per reaction. Molecular sizes were compared with a 50 bp molecular weight ladder (MW).</p

Agarose gel electrophoresis of : a) the PCR products from the Hexokinase (HK) genes of leishmanial reference strains after their double digestion with the endonucleases <i>MboI</i> and <i>HaeIII</i>, nd = not done; b) of PCR products from the HK genes of the leishmanial references and local Israeli and Palestinian strains, and Palestinian clinical samples after their double digestion with the endonucleases <i>MboI</i> and <i>HaeIII</i>.

<p>Uncut PCR product from <i>L. tropica</i> MHOM/PS/18JnMF4 ( = LRC-L891) was used as a control and molecular sizes were compared with a 50 bp molecular weight ladder (MW), nd = not done; c) of the PCR products from the Phosphoglucomutase (PGM) genes of the leishmanial reference strain <i>L. tropica</i> LRC-L863 and local Palestinian strains after single digestion with the endonuclease <i>MboI</i>. Only the heavier differentiating band is shown. Uncut PCR product from <i>L. tropica</i> MHOM/PS/02/79JnF20 ( = LRC-L885) was used as a control and molecular sizes were compared with a 50 bp molecular weight ladder (MW).</p

Leishmania guyanensis LgCL085 bin contig annotation files

Leishmania guyanensis LgCL085 bin contig annotation file

The HK and PGM genotypes of the strains of <i>Leishmania tropica</i> studied, listed by their WHO codes, in which M stands for mammal, HOM <i>Homo sapiens</i>, PRO the hyrax genus <i>Procavia</i>, I insect, SER the sand fly species <i>Phlebotomus sergenti</i>, ARA the sand fly species <i>Ph. arabicus</i>, ROS the sand fly species <i>Phlebotomus rossi</i>, EG Egypt, IL Israel, PS Palestine, KE Kenya, YE Yemen, NA Namibia, IQ Iraq, TR Turkey, IR Iran, AZ Azerbaijan, SY Syria, SU ex-Soviet Union, AF Afghanistan, MA Morocco, JO Jordan, IN India.

<p>The HK and PGM genotypes of the strains of <i>Leishmania tropica</i> studied, listed by their WHO codes, in which M stands for mammal, HOM <i>Homo sapiens</i>, PRO the hyrax genus <i>Procavia</i>, I insect, SER the sand fly species <i>Phlebotomus sergenti</i>, ARA the sand fly species <i>Ph. arabicus</i>, ROS the sand fly species <i>Phlebotomus rossi</i>, EG Egypt, IL Israel, PS Palestine, KE Kenya, YE Yemen, NA Namibia, IQ Iraq, TR Turkey, IR Iran, AZ Azerbaijan, SY Syria, SU ex-Soviet Union, AF Afghanistan, MA Morocco, JO Jordan, IN India.</p

Leishmania naiffi LnCL223 chromosome annotation files

Leishmania naiffi LnCL223 chromosome annotation file

a) Alignment of the HK nucleotide sequences of the strains of <i>Leishmania tropica</i>, <i>L. major</i> and <i>L. infantum</i>: cleavage sites of endonucleases MboI and HaeIII are ‘boxed’ in the HK sequence of the strains of <i>L. tropica</i>: HK-<i>Lt</i>G1 = <i>L. tropica</i> genotype 1 derived from strain ISER/IL/1998/LRC-L758; Lt = <i>L. tropica</i> DNA sequence, accession number = AY635845.1; HK-<i>Lt</i>G2 = <i>L. tropica</i> genotype 2 derived from strain MHOM/PS/2002/52JnYM18 ( = LRC-L890) Li = <i>L. infantum</i>, accession number = AY632240.1; Lm = <i>L. major</i>, accession number = FR796417.1.

<p>Arrows indicate positions of HK forward and reverse primers. b) Electropherograms of the sequences bestowing the <i>L. tropica</i> genotypes 1 and 2, HK-<i>Lt</i>G1 and HK-<i>Lt</i>G2, respectively. The pinpointed (↓) C at the bp 80 shows a double peak for (G–C) in the HK-<i>Lt</i>G2 electropherogram and should have been denoted by an S instead of the C. NN in the HK-<i>Lt</i>G2 electropherogram indicates (C) and (A) nucleotide, respectively.</p

Leishmania naiffi LnCL223 bin contig annotation files

Leishmania naiffi LnCL223 bin contig annotation file

Leishmania naiffi LnCL223 genome sequence files

Leishmania naiffi LnCL223 genome sequence file

Supplementary-Tables

Supplementary-Table

Figure-1-high-resolution

Figure-1-high-resolutio