Engineering Tools for the Estimation of Dredging-Induced Sediment Resuspension and Coastal Environmental Management

In recent years, increasing attention has been paid to environmental impacts that may result from resuspension, sedimentation and increase in concentration of chemicals during dredging activities. Dredging dislodges and resuspends bottom sediments that are not captured by dredge-head movements. Resuspended sediments are advected far from the dredging site as a dredging plume and the increase in the suspended solid concentration (SSC) can strongly differ, in time and space, depending on site and operational conditions. Well-established international guidelines often include numerical modelling applications to support environmental studies related to dredging activities. Despite the attention that has been focused on this issue, there is a lack of verified predictive techniques of plume dynamics at progressive distances from the different dredging sources, as a function of the employed dredging techniques and work programs, i.e., spatial and temporal variation of resuspension source. This chapter illustrates predictive techniques to estimate the SSC arising from dredges with different mechanisms of sediment release and to assess the spatial and temporal variability of the resulting plume in estuarine and coastal areas. Predictive tools are aimed to support technical choices during planning and operational phases and to better plan the location and frequency of environmental monitoring activities during dredging execution

HST survey of the Orion Nebula Cluster in the H2_2O 1.4 μ\mum absorption band: I. A census of substellar and planetary mass objects

In order to obtain a complete census of the stellar and sub-stellar population, down to a few M$_{Jup}$ in the $\sim1$ Myr old Orion Nebula Cluster, we used the infrared channel of the Wide Field Camera 3 of the Hubble Space Telescope with the F139M and F130N filters. These bandpasses correspond to the $1.4 \mu$m H$_2$O absorption feature and an adjacent line-free continuum region. Out of $4,504$ detected sources, $3,352$ (about $75\%$) appear fainter than m$_{130}=14$ (Vega mag) in the F130N filter, a brightness corresponding to the hydrogen-burning limit mass (M$\simeq 0.072 M_\odot$) at $\sim 1$ Myr. Of these, however, only $742$ sources have a negative F130M-139N color index, indicative of the presence of H$_2$O vapor in absorption, and can therefore be classified as bona-fide M and L dwarfs, with effective temperatures T$\lesssim 2850$ K at an assumed $1$ Myr cluster age. On our color-magnitude diagram, this population of sources with H$_2$O absorption appears clearly distinct from the larger background population of highly reddened stars and galaxies with positive F130M-F139N color index, and can be traced down to the sensitivity limit of our survey, m$_{130}\simeq 21.5$, corresponding to a $1$ Myr old $\simeq 3$M$_{Jup}$, planetary mass object under about 2 magnitudes of visual extinction. Theoretical models of the BT-Settl family predicting substellar isochrones of $1, 2$ and $3$ Myr (down to $\sim 1$M$_{Jup}$) fail to reproduce the observed H$_2$O color index at M$\lesssim 20$M$_{Jup}$. We perform a Bayesian analysis to determine extinction, mass and effective temperature of each sub-stellar member of our sample, together with its membership probability.Comment: Accepted for publication in the Astrophysical Journal. The resolution of several figures has been downgraded to comply with the size limit of arXiv submission

Before-During-After Biomonitoring Assessment for a Pipeline Construction in a Coastal Lagoon in the Northern Adriatic Sea (Italy)

During 2006–2008, a pipeline was buried in Vallona lagoon in the Northern Adriatic Sea (Italy). A Before-During-After environmental monitoring programme was scheduled to monitor possible alterations. Bioaccumulation of metal(loid)s, BTs (butyltins) and HMW-PAHs (High Molecular Weight Polycyclic Aromatic Hydrocarbons), and biological responses (Condition index, air Survival –LT50, Acetylcholinesterase, Micronuclei –MN, acyl-CoA oxidase, catalase, malondialdehyde –MDA, and the total oxyradical scavenging capacity-TOSCA) were investigated in Manila clams (Ruditapes philippinarum) from November 2005 to June 2015. In opera (IO) results showed higher levels of HMW-PAHs (73 ± 13 ng/g), BTs (90 ± 38 ng Sn/g) and increasing levels of Pb (6.7 ± 0.7 mg/kg) and Zn (73.6 ± 6.08 mg/kg) probably linked to works. Other contaminant alterations, especially metal(loid)s, before (AO) and after (PO) the burial, were attributed to a general condition of the area and mostly unrelated to works. In addition, LT50, MN and TOSCA showed alterations, probably due to hotspots occurring in IO. TOSCA and MDA increases, right after the burial, were considered delayed responses of IO, whilst other biological responses detected later were connected to the general condition of the area. Comparisons between results of Principal Component Analyses (PCAs) highlighted partial overlapping of AO and IO, whilst PO differed only for contaminants. Visual correlations between PCAs highlighted the biomarkers’ latter response

HST Survey of the Orion Nebula Cluster in the H₂O 1.4 μm Absorption Band. I. A Census of Substellar and Planetary-mass Objects

In order to obtain a complete census of the stellar and substellar population, down to a few M_(Jup) in the ~1 Myr old Orion Nebula Cluster, we used the infrared channel of the Wide Field Camera 3 of the Hubble Space Telescope with the F139M and F130N filters. These bandpasses correspond to the 1.4 μm H₂O absorption feature and an adjacent line-free continuum region. Out of 4504 detected sources, 3352 (about 75%) appear fainter than m₁₃₀ = 14 (Vega mag) in the F130N filter, a brightness corresponding to the hydrogen-burning limit mass M ≃ 0.072 M_⊙) at ~1 Myr. Of these, however, only 742 sources have a negative F130M–F139N color index, indicative of the presence of H₂O vapor in absorption, and can therefore be classified as bona fide M and L dwarfs, with effective temperatures T ≾ 2850 K at an assumed 1 Myr cluster age. On our color–magnitude diagram (CMD), this population of sources with H₂O absorption appears clearly distinct from the larger background population of highly reddened stars and galaxies with positive F130M–F139N color index and can be traced down to the sensitivity limit of our survey, m₁₃₀ ≃ 21.5, corresponding to a 1 Myr old ≃ 3 M_(Jup) planetary-mass object under about 2 mag of visual extinction. Theoretical models of the BT-Settl family predicting substellar isochrones of 1, 2, and 3 Myr down to ~1 M_(Jup) fail to reproduce the observed H₂O color index at M ≾ 20 M_(Jup). We perform a Bayesian analysis to determine extinction, mass, and effective temperature of each substellar member of our sample, together with its membership probability

Early downregulation of hsa-miR-144-3p in serum from drug-naïve Parkinson’s disease patients

Advanced age represents one of the major risk factors for Parkinson’s Disease. Recent biomedical studies posit a role for microRNAs, also known to be remodelled during ageing. However, the relationship between microRNA remodelling and ageing in Parkinson’s Disease, has not been fully elucidated. Therefore, the aim of the present study is to unravel the relevance of microRNAs as biomarkers of Parkinson’s Disease within the ageing framework. We employed Next Generation Sequencing to profile serum microRNAs from samples informative for Parkinson’s Disease (recently diagnosed, drug-naïve) and healthy ageing (centenarians) plus healthy controls, age-matched with Parkinson’s Disease patients. Potential microRNA candidates markers, emerging from the combination of differential expression and network analyses, were further validated in an independent cohort including both drug-naïve and advanced Parkinson’s Disease patients, and healthy siblings of Parkinson’s Disease patients at higher genetic risk for developing the disease. While we did not find evidences of microRNAs co-regulated in Parkinson’s Disease and ageing, we report that hsa-miR-144-3p is consistently down-regulated in early Parkinson’s Disease patients. Moreover, interestingly, functional analysis revealed that hsa-miR-144-3p is involved in the regulation of coagulation, a process known to be altered in Parkinson’s Disease. Our results consistently show the down-regulation of hsa-mir144-3p in early Parkinson’s Disease, robustly confirmed across a variety of analytical and experimental analyses. These promising results ask for further research to unveil the functional details of the involvement of hsa-mir144-3p in Parkinson’s Disease

Early downregulation of hsa-miR-144-3p in serum from drug-naïve Parkinson’s disease patients

open101siThis work was supported by the Horizon 2020 Framework Programme (Grant number 634821, PROPAG-AGING).Advanced age represents one of the major risk factors for Parkinson’s Disease. Recent biomedical studies posit a role for microRNAs, also known to be remodelled during ageing. However, the relationship between microRNA remodelling and ageing in Parkinson’s Disease, has not been fully elucidated. Therefore, the aim of the present study is to unravel the relevance of microRNAs as biomarkers of Parkinson’s Disease within the ageing framework. We employed Next Generation Sequencing to profile serum microRNAs from samples informative for Parkinson’s Disease (recently diagnosed, drug-naïve) and healthy ageing (centenarians) plus healthy controls, age-matched with Parkinson’s Disease patients. Potential microRNA candidates markers, emerging from the combination of differential expression and network analyses, were further validated in an independent cohort including both drug-naïve and advanced Parkinson’s Disease patients, and healthy siblings of Parkinson’s Disease patients at higher genetic risk for developing the disease. While we did not find evidences of microRNAs co-regulated in Parkinson’s Disease and ageing, we report that hsa-miR-144-3p is consistently down-regulated in early Parkinson’s Disease patients. Moreover, interestingly, functional analysis revealed that hsa-miR-144-3p is involved in the regulation of coagulation, a process known to be altered in Parkinson’s Disease. Our results consistently show the down-regulation of hsa-mir144-3p in early Parkinson’s Disease, robustly confirmed across a variety of analytical and experimental analyses. These promising results ask for further research to unveil the functional details of the involvement of hsa-mir144-3p in Parkinson’s Disease.openZago E.; Dal Molin A.; Dimitri G.M.; Xumerle L.; Pirazzini C.; Bacalini M.G.; Maturo M.G.; Azevedo T.; Spasov S.; Gomez-Garre P.; Perinan M.T.; Jesus S.; Baldelli L.; Sambati L.; Calandra Buonaura G.; Garagnani P.; Provini F.; Cortelli P.; Mir P.; Trenkwalder C.; Mollenhauer B.; Franceschi C.; Lio P.; Nardini C.; Adarmes-Gomez A.; Azevedo T.; Bacalini M.G.; Baldelli L.; Bartoletti-Stella A.; Bhatia K.P.; Marta B.-T.; Boninsegna C.; Broli M.; Dolores B.-R.; Calandra-Buonaura G.; Capellari S.; Carrion-Claro M.; Cilea R.; Clayton R.; Cortelli P.; Molin A.D.; De Luca S.; De Massis P.; Dimitri G.M.; Doykov I.; Escuela-Martin R.; Fabbri G.; Franceschi C.; Gabellini A.; Garagnani P.; Giuliani C.; Gomez-Garre P.; Guaraldi P.; Hagg S.; Hallqvist J.; Halsband C.; Heywood W.; Houlden H.; Huertas I.; Jesus S.; Jylhava J.; Labrador-Espinosa M.A.; Licari C.; Lio P.; Luchinat C.; Macias D.; Macri S.; Magrinelli F.; Rodriguez J.F.M.; Massimo D.; Maturo M.G.; Mengozzi G.; Meoni G.; Mignani F.; Milazzo M.; Mills K.; Mir P.; Mollenhauer B.; Nardini C.; Nassetti S.A.; Pedersen N.L.; Perinan-Tocino M.T.; Pirazzini C.; Provini F.; Ravaioli F.; Sala C.; Sambati L.; Scaglione C.L.M.; Schade S.; Schreglmann S.; Spasov S.; Strom S.; Tejera-Parrado C.; Tenori L.; Trenkwalder C.; Turano P.; Valzania F.; Ortega R.V.; Williams D.; Xumerle L.; Zago E.Zago E.; Dal Molin A.; Dimitri G.M.; Xumerle L.; Pirazzini C.; Bacalini M.G.; Maturo M.G.; Azevedo T.; Spasov S.; Gomez-Garre P.; Perinan M.T.; Jesus S.; Baldelli L.; Sambati L.; Calandra Buonaura G.; Garagnani P.; Provini F.; Cortelli P.; Mir P.; Trenkwalder C.; Mollenhauer B.; Franceschi C.; Lio P.; Nardini C.; Adarmes-Gomez A.; Azevedo T.; Bacalini M.G.; Baldelli L.; Bartoletti-Stella A.; Bhatia K.P.; Marta B.-T.; Boninsegna C.; Broli M.; Dolores B.-R.; Calandra-Buonaura G.; Capellari S.; Carrion-Claro M.; Cilea R.; Clayton R.; Cortelli P.; Molin A.D.; De Luca S.; De Massis P.; Dimitri G.M.; Doykov I.; Escuela-Martin R.; Fabbri G.; Franceschi C.; Gabellini A.; Garagnani P.; Giuliani C.; Gomez-Garre P.; Guaraldi P.; Hagg S.; Hallqvist J.; Halsband C.; Heywood W.; Houlden H.; Huertas I.; Jesus S.; Jylhava J.; Labrador-Espinosa M.A.; Licari C.; Lio P.; Luchinat C.; Macias D.; Macri S.; Magrinelli F.; Rodriguez J.F.M.; Massimo D.; Maturo M.G.; Mengozzi G.; Meoni G.; Mignani F.; Milazzo M.; Mills K.; Mir P.; Mollenhauer B.; Nardini C.; Nassetti S.A.; Pedersen N.L.; Perinan-Tocino M.T.; Pirazzini C.; Provini F.; Ravaioli F.; Sala C.; Sambati L.; Scaglione C.L.M.; Schade S.; Schreglmann S.; Spasov S.; Strom S.; Tejera-Parrado C.; Tenori L.; Trenkwalder C.; Turano P.; Valzania F.; Ortega R.V.; Williams D.; Xumerle L.; Zago E

Chemical-Physical Treatments Of Marine Contaminated Sediments – A Comparison

Managing of sediments coming from dredging operations in ports, harbor areas and navigation waterways has to deal with huge quantities of highly contaminated material. As a matter of fact, due to routine operations, to the need of deepening fairways and ports and, eventually, to remediation activities, every year more than 200·106 m3 of dredged materials are produced throughout Europe. Chemical-physical treatments are generally used in order to separate a contaminated fraction from a clean one in order to reduce the quantity of sediments to be disposed of. Within this research work, carried out by the Department of Chemical Material Environment Engineering of Sapienza University of Rome and ISPRA (High Institute for Environmental Protection and Research), former ICRAM, sediments coming from a harbor area characterized by metal contamination have been treated adopting three different technologies: sieving, hydro-cycloning and flotation. Results show that sieving, hydro-cycloning and flotation are able to separate products in which metal contaminants generally present lower concentration compared to that of untreated dredged sediments. Nevertheless, in order to further reduce metal content in the cleaned fraction, the examined treatment cannot stand alone as a single step, but a multi steps or a combination of treatments have to be considered