Neck fluid extravasation in shoulder arthroscopic surgery-a life-threatening complication: a report of two cases

Fluid extravasation in shoulder arthroscopy may lead to life-threatening consequences, such as airway compromise. These cases are rare and few were reported in the literature. Clinical cases of 2 female patients submitted to shoulder arthroscopy for a rotator cuff tear repair, complicated with fluid extravasation that led to trachea compression and need for intensive care unit (ICU) admission with postponed extubation. Neither patients showed any sequelae in the follow-up period. Report of these rare complications, with detailed and reproducible information, is vital for their comprehension and future elaboration of surveillance and treatment guidelines

Contribution to EPMA to airbone pollen analysis

As a component of aerosol, pollen is found in suspension with other mineralogical and anthropogenic particles that can adhere to the pollen wall. The aim of this study was to determine possible alterations that pollen grains suffer under different meteorological conditions in the coastal city of Porto, Portugal. For this study, 2 airborne pollen types were taken into account: Poaceae and Alnus spp sampled in July 2010 and February 2011, respectively. Quantitative analysis and X-ray map analysis were performed with a Field Emission Electron Probe Microanalyser (EPMA). Samples were mounted over adhesive carbon tape and carbon coated. Control pollen showed mainly the presence of C, O, N, P, K, Mg, S, Cl and Ca in different quantities, depending on the considered species. Airborne pollen consistently revealed a higher content of Mg and Cl than control pollen, while Si, Ca, K and P contents varied differently according to the samples. Our results show that during their “flight”, pollen grains acquire an external coating, become heavier and change significantly their composition

As experiências em proteção florestal da divisão florestal da Rigesa Meadwestvaco.

As experiências em proteção florestal da divisão florestal da Rigesa MeadWestvaco. A Divisão Florestal da Rigesa foi instalada em Três Barras SC em 1955 e atualmente possui unidades de manejo em 17 municípios no norte de Santa Catarina e sul do Paraná. A atividade florestal está voltada para o plantio, manejo e colheita de madeira plantada de Pinus e Eucalyptus. Na área de Proteção Florestal ao longo dos anos surgiram diversos desafios, para os quais a empresa tem criado ações específicas desenvolvidas por seu corpo técnico através de projetos cooperativos com Universidades e Instituições de Pesquisa, com o objetivo de encontrar a melhor solução para cada situação. Neste trabalho estão relatadas as últimas experiências e soluções encontradas pela empresa para os seguintes assuntos; Manejo Integrado do Pulgão-do- Pinus Cinara spp, detecção de Pissodes castaneus em florestas de Pinus; Cálculo da FMA ? Fórmula de Monte Alegre através da Estação Meteorológica Automática1 CD-ROM. Palestra (Pragas florestais). Co-promoção: Embrapa Florestas

Low-level laser therapy decreases local effects induced by myotoxins isolated from Bothrops jararacussu snake venom

The prominent myotoxic effects induced by Bothrops jararacussu crude venom are due, in part, to its polycationic myotoxins, BthTX-I and BthTX-II. Both myotoxins have a phospholipase A2 structure: BthTX-II is an active enzyme Asp-49 PLA2, while BthTX-I is a Lys-49 PLA2 devoid of enzymatic activity. In this study, the effect of low-level laser therapy (LLLT), 685 nm laser at a dose of 4.2 J/cm2 on edema formation, leukocyte influx and myonecrosis caused by BthTX-I and BthTX-II, isolated from Bothrops jararacussu snake venom, was analyzed. BthTX-I and BthTX-II caused a significant edema formation, a prominent leukocyte infiltrate composed predominantly by neutrophils and myonecrosis in envenomed gastrocnemius muscle. LLLT significantly reduced the edema formation, neutrophil accumulation and myonecrosis induced by both myotoxins 24 hours after the injection. LLLT reduced the myonecrosis caused by BthTX-I and BthTX-II, respectively, by 60 and 43%; the edema formation, by 41 and 60.7%; and the leukocyte influx, by 57.5 and 51.6%. In conclusion, LLLT significantly reduced the effect of these snake toxins on the inflammatory response and myonecrosis. These results suggest that LLLT should be considered a potential therapeutic approach for treatment of local effects of Bothrops species venom.Fundação Vale Paraibana de Ensin

Synthesis of bio-based Polyester from microbial lipidic residue intended for biomedical application

In the last decade, selectively tuned bio-based polyesters have been increasingly used for their clinical potential in several biomedical applications, such as tissue engineering, wound healing, and drug delivery. With a biomedical application in mind, a flexible polyester was produced by melt polycondensation using the microbial oil residue collected after the distillation of β-farnesene (FDR) produced industrially by genetically modified yeast, Saccharomyces cerevisiae. After characterization, the polyester exhibited elongation up to 150% and presented Tg of −51.2 °C and Tm of 169.8 °C. In vitro degradation revealed a mass loss of about 87% after storage in PBS solution for 11 weeks under accelerated conditions (40 °C, RH = 75%). The water contact angle revealed a hydrophilic character, and biocompatibility with skin cells was demonstrated. 3D and 2D scaffolds were produced by salt-leaching, and a controlled release study at 30 °C was performed with Rhodamine B base (RBB, 3D) and curcumin (CRC, 2D), showing a diffusion-controlled mechanism with about 29.3% of RBB released after 48 h and 50.4% of CRC after 7 h. This polymer offers a sustainable and eco-friendly alternative for the potential use of the controlled release of active principles for wound dressing applications.info:eu-repo/semantics/publishedVersio

SIMBA: a web tool for managing bacterial genome assembly generated by Ion PGM sequencing technology

Background The evolution of Next-Generation Sequencing (NGS) has considerably reduced the cost per sequenced-base, allowing a significant rise of sequencing projects, mainly in prokaryotes. However, the range of available NGS platforms requires different strategies and software to correctly assemble genomes. Different strategies are necessary to properly complete an assembly project, in addition to the installation or modification of various software. This requires users to have significant expertise in these software and command line scripting experience on Unix platforms, besides possessing the basic expertise on methodologies and techniques for genome assembly. These difficulties often delay the complete genome assembly projects. Results In order to overcome this, we developed SIMBA (SImple Manager for Bacterial Assemblies), a freely available web tool that integrates several component tools for assembling and finishing bacterial genomes. SIMBA provides a friendly and intuitive user interface so bioinformaticians, even with low computational expertise, can work under a centralized administrative control system of assemblies managed by the assembly center head. SIMBA guides the users to execute assembly process through simple and interactive pages. SIMBA workflow was divided in three modules: (i) projects: allows a general vision of genome sequencing projects, in addition to data quality analysis and data format conversions; (ii) assemblies: allows de novo assemblies with the software Mira, Minia, Newbler and SPAdes, also assembly quality validations using QUAST software; and (iii) curation: presents methods to finishing assemblies through tools for scaffolding contigs and close gaps. We also presented a case study that validated the efficacy of SIMBA to manage bacterial assemblies projects sequenced using Ion Torrent PGM. Conclusion Besides to be a web tool for genome assembly, SIMBA is a complete genome assemblies project management system, which can be useful for managing of several projects in laboratories. SIMBA source code is available to download and install in local webservers at http://ufmg-simba.sourceforge.net

Deposition and passage of transthyretin through the blood-nerve barrier in recipients of familial amyloid polyneuropathy livers

Familial amyloid polyneuropathy (FAP) is characterized by deposition of mutated transthyretin (TTR) in the peripheral nervous system. Prior to amyloid fibrils, nonfibrillar TTR aggregates are deposited inducing oxidative stress with increased nitration (3-NT). As the major source of TTR is the liver, liver transplantation (LT) is used to halt FAP. Given the shortage of liver donors, domino LT (DLT) using FAP livers is performed. The correlation between TTR deposition in the skin and nerve was tested in biopsies from normal individuals, asymptomatic carriers (FAP 0) and FAP patients; in FAP 0, nonfibrillar TTR was observed both in the skin and nerve in the same individuals; in patients, amyloid was detected in both tissues. The occurrence of amyloidosis in recipients of FAP livers was evaluated 1-7 years after DLT: TTR deposition occurred in the skin 3 years after transplantation either as amyloid or aggregates; in one of the recipients, fibrillar TTR was present in the epineurium 6 years after DLT. Deposits were scarce and 3-NT immunostaining was irrelevant. Nerve biopsies from DLT recipients had no FAP-related neuropathy. Our findings suggest that TTR amyloid formation occurs faster than predicted and that TTR of liver origin can cross the blood-nerve barrier. Recipients of FAP livers should be under surveillance for TTR deposition and tissue damag

Influência da glicerina bruta na cinética de fermentação ruminal in vitro.

A glicerina bruta (GB) gerada da produção de biodiesel do óleo de mamona pode ser uma opção para alimentação dos ruminantes. O objetivo deste trabalho foi avaliar os efeitos dos níveis (0, 0,5, 1,0, 2,0, 3,0 e 5,0% da matéria seca) de inclusão da GB oriunda do óleo de mamona utilizado para produção de biodiesel na fermentação in vitro do feno de braquiarão cv. Marandu (FM), do concentrado comercial (CC) e do feno mais concentrado (FC) na relação de 1:1. Foi utilizada a técnica in vitro semiautomática de produção de gases e os dados foram ajustados a um modelo unicompartimental. O potencial máximo de produção de gases variou de 207 (1% GB) a 234 mL (3% GB) para FM; de 266 (5% GB) a 329 mL (0% GB) para CC e 202 (5% GB) a 279 mL (0% GB) para FC. A taxa de produção de gases variou de 0,02 (3% GB) a 0,07 mL/h (1% GB) para FM; de 0,10 (1% GB) a 0,14 mL/h (5% GB) para CC e 0,05 (2, 3 e 5% GB) a 0,10 mL/h (0 e 0,5%GB) para FC. A glicerina aumentou o tempo de colonização do FM de 1h:40min (0% GB) para 3h:17min (3% GB) . A adição de GB teve efeito negativo nos parâmetros de cinética de produção de gases e digestibilidade. Estudos in vivo são necessários para avaliar a GB como substância gliconeogênica