Defects in Crystalline Packings of Twisted Filament Bundles: I. Continuum Theory of Disclinations

We develop the theory of the coupling between in-plane order and out-of-plane geometry in twisted, two-dimensionally ordered filament bundles based on the non-linear continuum elasticity theory of columnar materials. We show that twisted textures of filament backbones necessarily introduce stresses into the cross-sectional packing of bundles and that these stresses are formally equivalent to the geometrically-induced stresses generated in thin elastic sheets that are forced to adopt spherical curvature. As in the case of crystalline order on curved membranes, geometrically-induced stresses couple elastically to the presence of topological defects in the in-plane order. We derive the effective theory of multiple disclination defects in the cross section of bundle with a fixed twist and show that above a critical degree of twist, one or more 5-fold disclinations is favored in the elastic energy ground state. We study the structure and energetics of multi-disclination packings based on models of equilibrium and non-equilibrium cross-sectional order.Comment: 21 pages, 4 figure

A rational cubic spline with tension

A rational cubic spline curve is described which has tension control parameters for manipulating the shape of the curve. The spline is presented in both interpolatory and rational B-spline forms, and the behaviour of the resulting representations is analysed with respect to variation of the control parameters

OAST space research and technology applications: Technology transfer successes

The ultimate measure of success in the Space Research and Technology Program is the incorporation of a technology into an operational mission. Charts are presented that describe technology products which OAST has helped support that (1) have been used in a space mission, (2) have been incorporated into the baseline design of a flight system in the development phase, or (3) have been picked up by a commercial or other non-NASA user. We hope that these examples will demonstrate the value of investment in technology. Pictured on the charts are illustrations of the technology product, the mission or user which has incorporated the technology, and where appropriate, results from the mission itself

Filling the void in confined polymer nematics: phase transitions in a minimal model of dsDNA packing

Inspired to understand the complex spectrum of space-filling organizations the dsDNA genome within the capsid of bacterial viruses, we study a minimal, coarse-grained model of single chains densely-packed into a finite spherical volume. We build the three basic elements of the model--i) the absence of chain ends ii) the tendency of parallel-strand alignment and iii) a preference of uniform areal density of chain segments--into a polymer nematic theory for confined chains. Given the geometric constraints of the problem, we show that axially symmetric packings fall into one of three topologies: the coaxial spool; the simple solenoid; and the twisted-solenoid. Among these, only the twisted-solenoid fills the volume without the presence of line-like disclinations, or voids, and are therefore generically preferred in the incompressible limit. An analysis of the thermodynamics behavior of this simple model reveals a rich behavior, a generic sequence of phases from the empty state for small container sizes, to the coaxial spool configuration at intermediate sizes, ultimately giving way, via a second-order, symmetry-breaking transition, to the twisted-solenoid structure above a critical sphere size.Comment: 7 pages; 3 figure

Theory of Crosslinked Bundles of Helical Filaments: Intrinsic Torques in Self-Limiting Biopolymer Assemblies

Inspired by the complex influence of the globular crosslinking proteins on the formation of biofilament bundles in living organisms, we study and analyze a theoretical model for the structure and thermodynamics of bundles of helical filaments assembled in the presence of crosslinking molecules. The helical structure of filaments, a universal feature of biopolymers such as filamentous actin, is shown to generically frustrate the geometry of crosslinking between the "grooves" of two neighboring filaments. We develop a coarse-grained model to investigate the interplay between the geometry of binding and mechanics of both linker and filament distortion, and we show that crosslinking in parallel bundles of helical filaments generates {\it intrinsic torques}, of the type that tend to wind bundle superhelically about its central axis. Crosslinking mediates a non-linear competition between the preference for bundle twist and the size-dependent mechanical cost of filament bending, which in turn gives rise to feedback between the global twist of self-assembled bundles and their lateral size. Finally, we demonstrate that above a critical density of bound crosslinkers, twisted bundles form with a thermodynamically preferred radius that, in turn, increases with a further increase in crosslinking bonds. We identify the {\it stiffness} of crosslinking bonds as a key parameter governing the sensitivity of bundle structure and assembly to the availability and affinity of crosslinkers.Comment: 15 pages, 9 figures, Appendi