Study on Seed Dormancy and Germination Characteristics of Portulaca pilosa

To improve understanding of Portulaca pilosa seed germination and lay technical foundation for seedling cultivation, this paper studies the characteristics of dormancy and germination of Portulaca pilosa seed. Research indicates that its primary seed dormancy period is about 25 d, dormancy can be completely released by storage at room temperature for 30 d; the seed is positively photoblastic seed that can’t sprout and can be induced into dormancy in low light conditions; the dormancy-released seed is more sensitive to light when compared with the freshly picked seed, and the germination peak arises 1 d earlier; Portulaca pilosa seed favorites humid conditions, and the higher the soil moisture, the higher the germination rate and germination energy of the seed. The dormancy-released seeds should be used for Portulaca pilosa seedling cultivation, and after the germination is accelerated for 2-3 d in the light conditions, the seeds are sown on the soil surface, and covered with transparent plastic film to keep moisture and light, which is conducive to the emergence of seeds

Capturing the interactome of newly transcribed RNA

© 2018 Macmillan Publishers Limited, part of Springer Nature. All rights reserved. We combine the labeling of newly transcribed RNAs with 5-ethynyluridine with the characterization of bound proteins. This approach, named capture of the newly transcribed RNA interactome using click chemistry (RICK), systematically captures proteins bound to a wide range of RNAs, including nascent RNAs and traditionally neglected nonpolyadenylated RNAs. RICK has identified mitotic regulators amongst other novel RNA-binding proteins with preferential affinity for nonpolyadenylated RNAs, revealed a link between metabolic enzymes/factors and nascent RNAs, and expanded the known RNA-bound proteome of mouse embryonic stem cells. RICK will facilitate an in-depth interrogation of the total RNA-bound proteome in different cells and systems.Link_to_subscribed_fulltex