Optical Tweezers For Studying Taxis In Parasites

In this work we present a methodology to measure force strengths and directions of living parasites with an optical tweezers setup. These measurements were used to study the parasites chemotaxis in real time. We observed behavior and measured the force of: (i)Leishmania amazonensis in the presence of two glucose gradients; (ii)Trypanosoma cruzi in the vicinity of the digestive system walls, and (iii)Trypanosoma rangeli in the vicinity of salivary glands as a function of distance. Our results clearly show a chemotactic behavior in every case. This methodology can be used to study any type of taxis, such as chemotaxis, osmotaxis, thermotaxis, phototaxis, of any kind of living microorganisms. These studies can help us to understand the microorganism sensory systems and their response function to these gradients. © 2011 IOP Publishing Ltd.134DPDx-Trypanosomiasis, American. Fact Sheet. Centers for Disease Control (CDC). Retrieved 2008-09-11(2002), WHO, World Health OrganizationGrisard, E.C., Moraes, M.H., Guarneri, A.A., Girardi, F.P., Rodrigues, J.B., Eger-Mangrich, I., Tyler, K.M., Steindel, M., Different serological cross-reactivity of Trypanosoma rangeli forms in Trypanosoma cruzi-infected patients sera (2008) Parasites Vectors, 1 (1), p. 20Bagorda, A., Parent, C.A., Eukaryotic chemotaxis at a glance (2008) J. Cell Sci., 121 (16), pp. 2621-2624Teves, M.E., Guidobaldi, H.A., Ũates, D.R., Sanchez, R., Miska, W., Publicover, S.J., Morales Garcia, A.A., Giojalas, L.C., Molecular mechanism for human sperm chemotaxis mediated by progesterone (2009) PLoS ONE, 4 (12), p. 8211Snchez, R., Seplveda, C., Risopatrón, J., Villegas, J., Giojalas, L.C., Human sperm chemotaxis depends on critical levels of reactive oxygen species (2010) Fertil Steril., 93 (1), pp. 150-153Wu, J.Y., Feng, L., Park, H.-T., Havlioglu, N., Wen, L., Tang, H., Bacon, K.B., Rao, Y., The neuronal repellent Slit inhibits leukocyte chemotaxis induced by chemotactic factors (2001) Nature, 410 (6831), pp. 948-952. , DOI 10.1038/35073616Zheng, M., Sun, G., Cai, S., Mueller, R., Mrowietz, U., Significant reduction of T-cell chemotaxis to MCP-1 in patients with primary and metastatic melanoma (1999) Chin. Med. J. (Engl.), 112, pp. 493-496Kohidai, L., Chemotaxis: The proper physiological response to evaluate phylogeny of signal molecules (1999) Acta Biologica Hungarica, 50 (4), pp. 375-394Law, A.M.J., Aitken, M.D., Continuous-flow capillary assay for measuring bacterial chemotaxis (2005) Applied and Environmental Microbiology, 71 (6), pp. 3137-3143. , DOI 10.1128/AEM.71.6.3137-3143.2005Khan, S., Jain, S., Reid, G.P., Trentham, D.R., The fast tumble signal in bacterial chemotaxis (2004) Biophysical Journal, 86 (6), pp. 4049-4058. , DOI 10.1529/biophysj.103.033043Neuman, K.C., Chadd, E.H., Liou, G.F., Bergman, K., Block, S.M., Characterization of photodamage to Escherichia coli in optical traps (1999) Biophysical Journal, 77 (5), pp. 2856-2863Bleul, C.C., Farzan, M., Choe, H., Parolin, C., Clark-Lewis, I., Sodroski, J., Springer, T.A., The lymphocyte chemoattractant SDF-1 is a ligand for LESTR/fusin and blocks HIV-1 entry (1996) Nature, 382 (6594), pp. 829-833. , DOI 10.1038/382829a0Nagasawa, T., Hirota, S., Tachibana, K., Takakura, N., Nishikawa, S.-I., Kitamura, Y., Yoshida, N., Kishimoto, T., Defects of B-cell lymphopoiesis and bone-marrow myelopoiesis in mice lacking the CXC chemokine PBSF/SDF-1 (1996) Nature, 382 (6592), pp. 635-638. , DOI 10.1038/382635a0Nelson, R.D., Quie, P.G., Simmons, R.L., Chemotaxis under agarose-new and simple method for measuring chemotaxis and spontaneous migration of human polymorphonuclear leukocytes and monocytes (1975) J. Immunol., 115, pp. 1650-1656Blair, D.F., How bacteria sense and swim (1999) Annu. Rev. Microbiol., 49 (1), pp. 489-522Rao, C.V., Glekas, G.D., Ordal, G.W., The three adaptation systems of Bacillus subtilis chemotaxis (2008) Trends Microbiol., 16 (10), pp. 480-487Barros, V.C., Oliveira, J.S., Melo, M.N., Gontijo, N.F., Leishmania amazonensis: Chemotaxic and osmotaxic responses in promastigotes and their probable role in development in the phlebotomine gut (2006) Experimental Parasitology, 112 (3), pp. 152-157. , DOI 10.1016/j.exppara.2005.10.005, PII S0014489405002572Pfeffer, W., (1888) Unters. Botan. Inst. Tubingen, 2, pp. 582-661Adler, J., A method for measuring chemotaxis and use of the method to determine optimum conditions for chemotaxis by Escherichia coli (1973) J. Gen. Microbiol., 74, pp. 77-91Pozzo, L.Y., Fontes, A., De Thomaz, A.A., Santos, B.S., Farias, P.M., Ayres, D.C., Giorgio, S., Cesar, C.L., Studying taxis in real time using optical tweezers: Applications for Leishmania amazonensis parasites (2009) Micron, 40 (5-6), pp. 617-620Chagas, C., Nova tripanosomiase humana (1909) Mem. Inst. Oswaldo Cruz., 1, pp. 1-62Nogueira, N.F.S., Gonzales, M., Garcia, E.M., De Souza, W., Effect of azadirachtin A on the fine structure of the midgut of Rhodnius prolixus (1997) Journal of Invertebrate Pathology, 69 (1), pp. 58-63Gonzalez, M.S., Hamedi, A., Albuquerque-Cunha, J.M., Nogueira, N.F.S., De Souza, W., Ratcliffe, N.A., Azambuja, P., Mello, C.B., Antiserum against perimicrovillar membranes and midgut tissue reduces the development of Trypanosoma cruzi in the insect vector, Rhodnius prolixus (2006) Experimental Parasitology, 114 (4), pp. 297-304. , DOI 10.1016/j.exppara.2006.04.009, PII S0014489406001044Alves, C.R., Albuquerque-Cunha, J.M., Mello, C.B., Garcia, E.S., Nogueira, N.F., Bourguingnon, S.C., De Souza, W., Gonzalez, M.S., Trypanosoma cruzi: Attachment to perimicrovillar membrane glycoproteins of Rhodnius prolixus (2007) Experimental Parasitology, 116 (1), pp. 44-52. , DOI 10.1016/j.exppara.2006.11.012, PII S0014489406003171Gomes, S.A.O., Souza, A.L.F., Kiffer, T.M., Dick, C.F., Santos, A.L.A., Meyer-Fernandes, J.R., Ecto-phosphatase activity on the external surface of Rhodnius prolixus salivary glands: Modulation by carbohydrates and Trypanosoma rangeli (2008) Acta Trop., 106 (2), pp. 137-142Vallejo, G.A., Guhl, F., Schaub, G.A., Triatominae-Trypanosoma cruzi/T. rangeli: Vector-parasite interactions (2009) Acta Trop., 110 (2-3), pp. 137-147Happel, J., Brenner, H., (1991) Low Reynolds Number Hydrodynamics with Special Applications to Particulate Media(1971) Handbook of Chemistry and PhysicsDe Thomaz, A.A., Optical tweezers force measurements to study parasites chemotaxis (2009) Proc. SPIE, 7367, pp. 73671