Gestão da qualidade na Embrapa Clima Temperado.

bitstream/item/41092/1/documento-294.pd

Identificação e clonagem de promotores raiz-específicos de eucalipto.

A utilização de promotores tecido - específicos é uma das principais estratégias para direcionar a expres são de transgenes a determinados órgãos ou tipos celulares . Por isso, o objetivo deste trabalho foi a clonagem de promotores raiz - específicos , a partir de dados de RNA - Seq obtidos de bibliotecas de raiz, xilema e folha de eucalipto. Dois genes com padrão d e expressão preferencial em raiz foram inicialmente selecionados para isolamento da região promotora. Fragmentos de 1kb foram clonados em vetor binário pCAMBIA2301, fusionados ao gene repórter da beta - glucuronidase (GUS) , para transformação de Populus trem ula x Populus alba via Agrobacterium tumefaciens .Edição dos anais do 3º Simpósio de Bioquímica e Biotecnologia, 2013. Londrina

Manual de biossegurança em laboratórios da Embrapa Florestas.

bitstream/item/24481/1/Doc201.pd

Light Element Abundances From z=0 To z=5

Plausible ratios of deuterium to hydrogen D/H as a function of metallicity, time, and redshift are investigated. Guided by the heavy element abundance patterns observed locally in Galactic dwarf stars and at large redshift in quasi-stellar object absorption line systems, empirical evolution of the relative abundance ratios Li/D, B/D, N/D, O/D, and F/D for QSO absorption line systems are given for the possible evolutionary patterns in D/H. Shortened abstract.Comment: 30 pages including 8 figures, ApJ in pres

Flame made ceria supported noble metal catalysts for efficient H₂ production via the water gas shift reaction

Rh/ceria catalysts were synthesized by flame spray pyrolysis for high temperature water gas shift (WGS) reactions. These catalysts show a high specific surface area due to a high degree of nanocrystallinity. X-ray absorption spectroscopy (XAS) unraveled the formation of small Rh particles under WGS reaction conditions. The catalytic activity was examined at atmospheric pressure by measuring CO conversion as a function of temperature. Some methane formation was observed above 310 °C

Background discrimination capabilities of a heat and ionization germanium cryogenic detector

The discrimination capabilities of a 70 g heat and ionization Ge bolometer are studied. This first prototype has been used by the EDELWEISS Dark Matter experiment, installed in the Laboratoire Souterrain de Modane, for direct detection of WIMPs. Gamma and neutron calibrations demonstrate that this type of detector is able to reject more than 99.6% of the background while retaining 95% of the signal, provided that the background events distribution is not biased towards the surface of the Ge crystal. However, the 1.17 kg.day of data taken in a relatively important radioactive environment show an extra population slightly overlapping the signal. This background is likely due to interactions of low energy photons or electrons near the surface of the crystal, and is somewhat reduced by applying a higher charge-collecting inverse bias voltage (-6 V instead of -2 V) to the Ge diode. Despite this contamination, more than 98% of the background can be rejected while retaining 50% of the signal. This yields a conservative upper limit of 0.7 event.day^{-1}.kg^{-1}.keV^{-1}_{recoil} at 90% confidence level in the 15-45 keV recoil energy interval; the present sensitivity appears to be limited by the fast ambient neutrons. Upgrades in progress on the installation are summarized.Comment: Submitted to Astroparticle Physics, 14 page