Isolation And Characterization Of Y Chromosome Dna Probes

A sorted, cloned Y chromosome phage library was screened for unique Y chromosome sequences. Of the thousands of plaques screened, 13 did not hybridize to radiolabeled 46, XX total chromosomal DNA. Three plaques were characterized further. Clone Y1 hybridized to multiple restriction enzyme fragments in both male and female DNA with more intense bands in male DNA. Clone Y2, also found in female and male DNA, is probably located in the pseudosutosomal region because extra copies of either the X or Y chromosomes increased Y2 restriction enzyme fragment intensity in total cellular DNA. Clone Y5 was male specific in three of four restriction enzyme digests althouth in the fourth a light hybridizing band was observed in both male and female DNA. Clone Y5 was sublocalized to band Yq 11.22 by hybridization to a panel of cellular DNA from patients with Y chromosome rearrangements. Clone Y5 can be used to test for retention of the proximally long arm Y suggested to cause gonadal cancer in carrier females. The long series of GA repeats in Y5, anticipated to be polymorphic, may provide a sensitive means to follow Y chromosome variation in human populations. © 1992.1891581589Schonberg, (1989) Handbook of Human Growth and Developmental Biology, 2, pp. 229-237. , 2nd Edition, E. Meisami, P. Timivas, CRC Press, Boca Raton, FLPage, de la Chapelle, Weissenbach, (1985) Nature, 315, pp. 224-226Disteche, Casanova, Saal, Friedman, Sybert, Graham, Thuline, Page, (1986) PNAS, 83, pp. 7841-7844Vergnaud, Page, Simmler, Brown, Rouyer, Noel, Botstein, Weissenbach, (1986) Am. J. Hum. Genet, 38, pp. 109-124McLaren, Simpson, Tomonari, Chandler, Hogg, (1984) Nature, 312, pp. 552-555Simpson, Chandler, Goulmy, Disteche, Ferguson-Smith, Page, (1987) Nature, 326, pp. 876-878Koopman, Gubbay, Vivian, Goodfellow, Lovell-Badge, (1991) Nature, 351, pp. 117-121Washburn, Eicher, (1983) Nature, 303, pp. 338-34015Rouyer, Simmler, Johnsson, Vergnaud, Cooke, Weissenbach, (1986) Nature, 319, pp. 291-295Yen, Marsh, Allen, Taai, Ellison, Connolly, Neiswanger, Shapiro, (1988) Cell, 55, pp. 1123-1138de la Chapelle, The etiology of maleness in XX men (1981) Human Genetics, 58, pp. 105-116Page, Mosher, Simpson, Fisher, Mardon, Pollack, McGillivray, Brown, The sex-determining region of the human Y chromosome encodes a finger protein (1987) Cell, 51, pp. 1091-1104Lau, (1984) The Y Chromosome, Part A: Basic Characteristics of the Y Chromosome, pp. 177-192. , A. Sandberg, Alan R. Liss, New YorkNgo, Vergnaud, Johnsson, Lucotte, Weissenbach, (1986) Am. J. Hum. Genet, 38, pp. 407-418Wolman, David, Koo, (1985) The Y chromosome. Part A: basic characteristics of the Y chromosome, pp. 477-505. , A. Sandberg, Alan R. Liss, NYGemmill, Pearce-Birge, Bixenman, Hecht, Allanson, (1987) Am. J. Hum. Genet, 41, pp. 157-167Goosens, Kan, [49] DNA analysis in the diagnosis of hemoglobin disorders (1981) Method. Enzymol, 76, pp. 805-817Van Dilla, Deaven, Construction of gene libraries for each human chromosome (1990) Cytometry, 11, pp. 208-218Sambrook, Fritsch, Maniatis, (1989) Molecular Cloning: a Laboratory Manual, , 2nd Edition, Cold Spring Harbor Press, NYRigby, Dieckmann, Rhodes, Berg, (1977) J. Mol. Biol, 113, pp. 237-251Feinberg, Vogelstein, (1984) Anal. Biochem, 137, pp. 266-267Farah, Garmes, Cavalcanti, Mello, Porelli, Ramos, Sartorato, (1991) Brazilian J. Med. Biol. Res, 24, pp. 149-156Southern, (1975) J. Mol. Biol, 98, pp. 503-51

Gaucher disease: Current issues in diagnosis and treatment

FWN – Publicaties zonder aanstelling Universiteit Leide