Jaccoud arthropathy in systemic lupus erythematosus: clinical and serological findings

SummaryObjectiveTo study the prevalence of Jaccoud arthropathy (JA) in a sample of local systemic lupus erythematosus (SLE) patients and its clinical and serological associations.Methods308 SLE patients from a single university center for the last two years were interviewed and examined. The presence of JA was searched for according to the JA index. After this, charts were reviewed for clinical and serological profile.ResultsThe studied sample was composed by 94.5% females and 5.5% males with mean age of 38.08±12.04 years and mean disease duration of 29.68±11.63 years. A JA prevalence of 6.1% was found in this sample. There was a positive association of JA presence with arthritis complaints (p=0.001) and a negative association with renal involvement (p=0.028). Patients with JA had higher positivity for anti-dsDNA (p=0.022).ConclusionDespite the positive association of JA with arthritis and anti-dsDNA, there was a negative association with nephritis. This could suggest that JA patients belong with a SLE subset with a better prognosis

From moths to caterpillars: Ideal conditions for Galleria mellonella rearing for in vivo microbiological studies.

ABSTRACT Galleria mellonella is a well-accepted insect model for the study of pathogen-host interactions and antimicrobial compounds. The main advantages of this model include the low cost of maintenance, the fast life cycle, the possibility of using a large number of caterpillars and the innate immune system, which is evolutionarily conserved relative to mammals. Because of these advantages, different research groups have been working to implement the rearing of G. mellonella in laboratory conditions. This protocol describes our experience in the rearing of G. mellonella caterpillars for experimental infection models and the influence of different artificial diets on developmental and physiological parameters. Here, we suggest a diet composition that benefits the life cycle of G. mellonella by accelerating the larval phase length and increasing the caterpillar weight. This diet also stimulated the immune system of G. mellonella by increasing the hemolymph volume and hemocyte concentration. In addition, our rearing protocol generated caterpillars that are more resistant to infection by Staphylococcus aureus, Escherichia coli and Candida albicans. A standard G. mellonella rearing protocol is fundamental to minimize external influences on the results, and this simple and easy protocol can support researchers starting to rear G. mellonella

Single-particle characterization of aerosols collected at a remote site in the Amazonian rainforest and an urban site in Manaus, Brazil

In this study, aerosol samples collected at a remote site in the Amazonian rainforest and an urban site in Manaus, Brazil, were investigated on a single-particle basis using a quantitative energy-dispersive electron probe X-ray microanalysis (ED-EPMA). A total of 23 aerosol samples were collected in four size ranges (0.25–0.5, 0.5–1.0, 1.0–2.0, and 2.0–4.0&thinsp;µm) during the wet season in 2012 at two Amazon basin sites: 10 samples in Manaus, an urban area; and 13 samples at an 80&thinsp;m high tower, located at the Amazon Tall Tower Observatory (ATTO) site in the middle of the rainforest, 150&thinsp;km northeast of Manaus. The aerosol particles were classified into nine particle types based on the morphology on the secondary electron images (SEIs) together with the elemental concentrations of 3162 individual particles: (i) secondary organic aerosols (SOA); (ii) ammonium sulfate (AS); (iii) SOA and AS mixtures; (iv) aged mineral dust; (v) reacted sea salts; (vi) primary biological aerosol (PBA); (vii) carbon-rich or elemental carbon (EC) particles, such as soot, tarball, and char; (viii) fly ash; and (ix) heavy metal (HM, such as Fe, Zn, Ni, and Ti)-containing particles. In submicron aerosols collected at the ATTO site, SOA and AS mixture particles were predominant (50&thinsp;%–94&thinsp;% in relative abundance) with SOA and ammonium sulfate comprising 73&thinsp;%–100&thinsp;%. In supermicron aerosols at the ATTO site, aged mineral dust and sea salts (37&thinsp;%–70&thinsp;%) as well as SOA and ammonium sulfate (28&thinsp;%–58&thinsp;%) were abundant. PBAs were observed abundantly in the PM2−4 fraction (46&thinsp;%), and EC and fly ash particles were absent in all size fractions. The analysis of a bulk PM0.25−0.5 aerosol sample from the ATTO site using Raman microspectrometry and attenuated total reflection Fourier-transform infrared spectroscopy (ATR-FTIR) showed that ammonium sulfate, organics, and minerals are the major chemical species, which is consistent with the ED-EPMA results. In the submicron aerosols collected in Manaus, either SOA and ammonium sulfate (17&thinsp;%–80&thinsp;%) or EC particles (6&thinsp;%–78&thinsp;%) were dominant depending on the samples. In contrast, aged mineral dust, reacted sea salt, PBA, SOA, ammonium sulfate, and EC particles comprised most of the supermicron aerosols collected in Manaus. The SOA, ammonium sulfate, and PBAs were mostly of a biogenic origin from the rainforest, whereas the EC and HM-containing particles were of an anthropogenic origin. Based on the different contents of SOA, ammonium sulfate, and EC particles among the samples collected in Manaus, a considerable influence of the rainforest over the city was observed. Aged mineral dust and reacted sea-salt particles, including mineral dust mixed with sea salts probably during long-range transatlantic transport, were abundant in the supermicron fractions at both sites. Among the aged mineral dust and reacted sea-salt particles, sulfate-containing ones outnumbered those containing nitrates and sulfate&thinsp;+&thinsp;nitrate in the ATTO samples. In contrast, particles containing sulfate&thinsp;+&thinsp;nitrate were comparable in number to particles containing sulfate only in the Manaus samples, indicating the different sources and formation mechanisms of secondary aerosols, i.e., the predominant presence of sulfate at the ATTO site from mostly biogenic emissions and the elevated influences of nitrates from anthropogenic activities at the Manaus site.</p

Intake, digestibility, nitrogen balance and performance in lamb fed spineless cactus silage associated with forages adapted to the semiarid environment Spineless cactus silages in diets for lambs.

The study aimed to evaluate the intake, apparent digestibility, nitrogen balance and productive performance in lamb fed spineless cactus silage associated with forages adapted to the semiarid environment. Forty intact crossbred lambs, with an average body weight of 22.65 ± 1.01 kg, were distributed in a completely randomised design with five treatments (diets based on: spineless cactus silage (SCS), spineless cactus + buffelgrass silage (SCBS), spineless cactus + gliricidia silage (SCGS), spineless cactus + pornunça silage (SCPS) and corn silage (CS; witness) and eight animals per treatment. Intake, apparent nutrient digestibility, nitrogen balance, and performance of animals were evaluated. The SCGS diet showed higher intake of dry matter and organic matter (P<0.05). The SCGS and SCPS diets showed the highest crude protein intake (P = 0.005). Lower intake of ether extract was found in animals receiving diets containing SCS and SCBS (P = 0.001). SCBS and SCGS provided a higher intake of neutral detergent fiber (P = 0.015). SCS and SCGS diets displayed a higher intake of non-fiber carbohydrates (P = 0.003). SCGS diets displayed the highest total digestible nutrients intake (P = 0.001). SCPS diet showed lower digestibility of dry matter, organic matter, and crude protein (P<0.05). SCGS diet showed a lower digestibility coefficient for ether extract (P = 0.009). SCPS diet showed lower digestibility coefficients for neutral detergent fiber (P = 0.022). SCS and SCPS diets showed lower digestibility coefficients for non-fiber carbohydrates (P = 0.011). The CS promoted lower results for intake (P = 0.003), absorbed (P = 0.003) and balance (P = 0.012) for nitrogen. SCPS diet promoted higher excretion of nitrogen via faeces (P = 0.001) and less excretion of nitrogen via urine (P = 0.033). SCGS diet provided a higher final weight (P = 0.006). SCS and SCGS diets provided more significant body weight gain (P = 0.001). Combining spineless cactus with a roughage base allowed weight gains over 200 g/day, proving to be efficient in diets for feedlot lamb and gains similar or superior to corn-based diets

Water intake and ingestive behavior of sheep fed diets based on silages of cactus pear and tropical forages.

The objective was to evaluate the water intake and ingestive behavior of sheep fed diets containing silages of cactus pear combined with tropical forages. Forty sheep without defined breed, intact, with initial average weight of 22.65 ± 1.01 kg were distributed in a completely randomized design with 5 treatments and 8 replications. The experimental diets consisted of cactus pear silage (CPS), cactus pear + buffel grass silage (CPBS), cactus pear + gliricidia silage (CPGS), cactus pear + pornunça silage (CPPS), and corn silage (CS). CPGS provided higher water intake via food, total water intake, metabolic water, and excretion via feces and urine (P < 0.05). Animals that received diets containing CS showed higher water intake via drinking fountain, less efficient feeding and rumination of dry matter, less efficient rumination of neutral detergent fiber, grams of dry matter per cud, grams of neutral detergent fiber per cud, and the shortest average time spent in chewing per cud (P < 0.05). CPGS, CPPS, and CS provided longer times for rumination and numbers of cuds per day (P < 0.05). CPS showed animals spending more time in idleness, lower quantity of cuds per minute, higher concentration of crystals in urine, with a higher frequency of ammonia-magnesium phosphate and calcium oxalate. Silages based on cactus pear are an alternative to the supply of water via food for sheep in semi-arid

Techonolgy of Qualea grandiflora Mart. (Vochysiaceae) seeds

Qualea grandiflora Mart. (Vochysiaceae), commonly known as "pau-terra", is an arborous species native to the Brazilian savannah which possess commercial interests, as it can be used either as an ornamental or as a medicinal plant. "Pau-terra" can also be used in the heterogeneous reforestation of areas which are destined for restoration of permanent preservation degraded areas. Propagation studies with this species are scarce, being necessary then further clarification regarding the factors that influences the germination process. In this context, the objective of this work was to evaluate the influence of different temperatures, substrates and light conditions on seed germination. We selected light brown seeds which were subjected to different interactions between temperatures (15-25, 20-30, 25 and 30°C), substrate (paper, sand and vermiculite) and light (light and dark). All seeds were later dry-incubated at 32°C for 3, 6 and 12 hours. After treatments, seeds were kept in BOD at 58% RH and the following parameters were calculated: germination (%G) and germination speed index (GSI); the formation of normal and abnormal seedlings and the number dead seeds. Interaction was observed for all variables. In the optimum temperature range, the seeds behaved as photoblastic neutral or indifferent. Under alternating temperatures, darkness enhanced the germination, especially when combined with the lower temperatures. We noted that the sowing in sand, at 25°C, allowed the maintenance of suitable combinations of germination and seedling development. With respect to desiccation tolerance, "pau-terra" seeds presented an orthodox behavior, with a linear increase of the vigor as function of drying

Net Charge Fluctuations in Au + Au Interactions at sqrt(s_NN) = 130 GeV

Data from Au + Au interactions at sqrt(s_NN) = 130 GeV, obtained with the PHENIX detector at RHIC, are used to investigate local net charge fluctuations among particles produced near mid-rapidity. According to recent suggestions, such fluctuations may carry information from the Quark Gluon Plasma. This analysis shows that the fluctuations are dominated by a stochastic distribution of particles, but are also sensitive to other effects, like global charge conservation and resonance decays.Comment: 6 pages, RevTeX 3, 3 figures, 307 authors, submitted to Phys. Rev. Lett. on 21 March, 2002. Plain text data tables for the points plotted in figures for this and previous PHENIX publications are (will be made) publicly available at http://www.phenix.bnl.gov/phenix/WWW/run/phenix/papers.htm

Methods, fluxes and sources of gas phase alkyl nitrates in the coastal air

The daily and seasonal atmospheric concentrations, deposition fluxes and emission sources of a few C3-C9 gaseous alkyl nitrates (ANs) at the Belgian coast (De Haan) on the Southern North Sea were determined. An adapted sampler design for low- and high-volume air-sampling, optimized sample extraction and clean-up, as well as identification and quantification of ANs in air samples by means of gas chromatography mass spectrometry, are reported. The total concentrations of ANs ranged from 0.03 to 85 pptv and consisted primarily of the nitro-butane and nitro-pentane isomers. Air mass backward trajectories were calculated by the Hybrid Single-Particle Lagrangian Integrated Trajectory (HYSPLIT) model to determine the influence of main air masses on AN levels in the air. The shorter chain ANs have been the most abundant in the Atlantic/Channel/UK air masses, while longer chain ANs prevailed in continental air. The overall mean N fluxes of the ANs were slightly higher for summer than those for winter-spring, although their contributions to the total nitrogen flux were low. High correlations between AN and HNO2 levels were observed during winter/spring. During summer, the shorter chain ANs correlated well with precipitation. Source apportionment by means of principal component analysis indicated that most of the gas phase ANs could be attributed to traffic/combustion, secondary photochemical formation and biomass burning, although marine sources may also have been present and a contributing factor. © 2014 Springer International Publishing Switzerland