Remote Indigenous housing procurement and post-occupancy outcomes: a comparative study

James Davidson, Carroll Go-Sam and Paul Memmot

Using Working Models of Attachment to Understand Paranoia in the General Population

The focus of this thesis is on paranoia and the factors that are thought to influence its development and maintenance. This thesis consists of three parts. Part one presents a systematic literature review on the evidence for a hostile attribution bias in paranoia. No systematic review has yet considered the extent to which this social cognitive bias is associated with paranoia. The evidence for this relationship is considered in the context of both clinical and non-clinical populations. The results supported hostility bias as being significantly associated with the specific experience of paranoia, rather than the broad diagnosis of psychosis. Part two is an empirical paper on attachment style as a predictor of paranoia in a non-clinical population. The results supported the relationship between paranoia and hostility bias, as expected, but in the context of attachment style this relationship was no longer significant. As per our hypotheses, attachment anxiety and attachment avoidance were both highly significant predictors of paranoia, suggesting that hostility bias was an expression of attachment style. Findings are discussed in relation to the evidence base, new directions for future research and clinical practice recommendations. Part three is a critical appraisal discussing conceptual issues, development of the methodology and reflections on the research proces

Building new housing in remote Indigenous communities

SOCIAL AND ECONOMIC BENEFITS CAN ACCOMPANY CAPITAL INVESTMENT IN HOUSING AND INFRASTRUCTURE. THESE ARE MORE LIKELY TO BE REALISED WHEN CONTRACTING METHODS FOR HOUSING PROCUREMENT ARE ALIGNED TO COMMUNITY NEEDS. ALLIANCE CONTRACTING, WHERE RISKS ARE SHARED, CAN SUPPORT INNOVATION IN THE PROCUREMENT SYSTEM TO ACHIEVE ADDITIONAL SOCIAL AND ECONOMIC BENEFITS

Impact of Value Added Tax towards GDP growth

本研究的主要目标是为了探讨是否引进增值税对促进GDP增长有十分重要的角色.为了回答这个问题，来自年1980-2014的IMF世界经济数据用于进行这份研究工作.通过多元回归分析方法来计算此影响.结果表明，引进增值税对大约4%的GDP增长的积极且在统计上显著的影响.我们还发现，在一个百分点的增值税率由3%增加了长期经济增长.最后，我们发现引进增值税增加了发展中国家的经济增长比发达国家更多的约五倍.The main objective of this study is to explore whether the introduction of a value added tax (VAT) is significant in promoting GDP growth. To answer this question, data taken from the World Economic Output of the IMF from years 1980-2014 were used in carrying out this research work. Multiple regressions methodology was employed to calculate this impact. The results revealed that the introduction o...学位：经济学硕士院系专业：经济学院_国际商务硕士学号：1522012115420

Mechanistic Insights into Hsp104 Potentiation

Potentiated variants of Hsp104, a protein disaggregase from yeast, can dissolve protein aggregates connected to neurodegenerative diseases such as Parkinson disease and amyotrophic lateral sclerosis. However, the mechanisms underlying Hsp104 potentiation remain incompletely defined. Here, we establish that 2–3 subunits of the Hsp104 hexamer must bear an A503V potentiating mutation to elicit enhanced disaggregase activity in the absence of Hsp70. We also define the ATPase and substrate-binding modalities needed for potentiated Hsp104A503V activity in vitro and in vivo. Hsp104A503V disaggregase activity is strongly inhibited by the Y257A mutation that disrupts substrate binding to the nucleotide-binding domain 1 (NBD1) pore loop and is abolished by the Y662A mutation that disrupts substrate binding to the NBD2 pore loop. Intriguingly, Hsp104A503V disaggregase activity responds to mixtures of ATP and adenosine 5′-(γ-thio)-triphosphate (a slowly hydrolyzable ATP analogue) differently from Hsp104. Indeed, an altered pattern of ATP hydrolysis and altered allosteric signaling between NBD1 and NBD2 are likely critical for potentiation. Hsp104A503V variants bearing inactivating Walker A or Walker B mutations in both NBDs are inoperative. Unexpectedly, however, Hsp104A503V retains potentiated activity upon introduction of sensor-1 mutations that reduce ATP hydrolysis at NBD1 (T317A) or NBD2 (N728A). Hsp104T317A/A503V and Hsp104A503V/N728A rescue TDP-43 (TAR DNA-binding protein 43), FUS (fused in sarcoma), and α-synuclein toxicity in yeast. Thus, Hsp104A503V displays a more robust activity that is unperturbed by sensor-1 mutations that greatly reduce Hsp104 activity in vivo. Indeed, ATPase activity at NBD1 or NBD2 is sufficient for Hsp104 potentiation. Our findings will empower design of ameliorated therapeutic disaggregases for various neurodegenerative diseases

770-5 Chamber Specific Regulation of the Sarcoplasmic Reticulum Calcium ATPase Pump In Human Heart Failure

Alterations in the expression of Ca2+ channels have been described in failing human left ventricle, including down regulation of the ryanodine receptor (RyR)/Ca2+ release channel and the sarcoplasmic reticulum Ca2+ ATPase pump (SERCA) which are involved in excitation-contraction coupling and relaxation (Cir Res 71: 18, 1992). We previously reported chamber specific regulation of the RyR during end-stage human heart failure (Clin Res 42(2):166A. 1994). We investigated whether SERCA is also regulated in the other cardiac chambers during human heart failure. Total RNA and protein homogenates were isolated from the left and right atria (LA, RA) and left and right ventricles (LV, RV) obtained prospectively from 32 cardiac transplant patients and 4 normal controls. Messenger RNA (mRNA) levels of SERCA were quantified using Northern and slot blot hybridizations with a 1.6kb rat cardiac SERCA cDNA probe and normalized to 28S ribosomal levels. Protein levels of SERCA were quantified using enzyme-linked immunosorbent assays with monoclonal antibodies directed against dog cardiac SERCA. Northern analyses detected a single ≈4 kb mRNA in all regions. Compared to controls. SERCA mRNA expression in failing hearts was decreased in LV by 39% (p<0.005), unchanged in RV, and increased in LA by 255% (p<0.005) and in RA by 338% (p<0.025). Consistent with the mRNA data. immunodetectable levels of SERCA were also reduced in LV by 30% (p<0.05) and unchanged in RV; however, protein levels appeared unchanged or reduced in both atria in contrast to the mRNA. This is the first study reporting simultaneous measurements of SERCA mRNA and protein levels in the human heart. We conclude that chamber specific regulation of SERCA mRNA occurs during end-stage heart failure. corroborated by protein expression in the ventricles. Down regulations of SERCA may contribute to impaired relaxation and increased diastolic tone during heart failure

Rainfall Stimulation of Primary Production in Western Atlantic Ocean Waters: Roles of Different Nitrogen Sources and Co-Limiting Nutrients

Using shipboard bioassays, we examined the roles rainfall, individual and combined nutrients play in accelerating primary production in coastal, Gulf Stream and pelagic (Sargasso Sea) locations in the North Atlantic Ocean off North Carolina, USA, from 1993 to 1995. Photosynthetic CO2 fixation and net chlorophyll a (chl a) production were measured In replicated bioassays to assess individual and combined impacts of different constituents of atmospheric deposition, including natural rainfall, a synthetic rain mix, dissolved inorganic nitrogen (DIN; NH4+ ,NO3-), dissolved organic nitrogen (DON; urea),phosphorus (PO43-) and iron (as EDTA-chelated and unchelated FeCl3).Natural rainfall and DIN additions most often stimulated CO2 fixation and chl a production, but frequencies and magnitudes of biostimulation, relative to controls, varied between these indicators. Spatial differences in the types and magnitudes of stimulation were also observed. When added in equimolar amounts, NH4+ was, at times, more stimulatory than NO3-. The NO3- stimulation was significantly enhanced by Fe-EDTA. Urea was marginally stimulatory at the coastal location. PO43- was never stimulatory. Fe-EDTA and EDTA by themselves stimulated production only at the offshore locations, suggesting increased Fe limitation with increasing distance from land. Synthetic rain, which contained both sources of DIN, but not Fe, generally proved less stimulatory per unit N than natural rainfall. Results indicate a broad sensitivity of these waters to N additions, which in the case of NO3- are enhanced by Fe-EDTA. At all locations, the high level of stimulation of primary production attributable to natural rain may be due to the supply of both DIN and CO-limiting nutrients (e.g. Fe), contributing to the eutrophication potential of waters downwind of urban, industrial and agricultural emissions