11 research outputs found
Bovine pericardium retail preserved in glutaraldehyde and used as a vascular patch
<p>Abstract</p> <p>Background</p> <p>In this study we evaluated the performance of bovine pericardium preserved in glutaraldehyde used as a vascular patch.</p> <p>Methods</p> <p>Fourteen young pigs, six females and eight males, weighting 10.3 - 18.4 kg were used in our study. We implanted three remnants in each pig, two in the abdominal aorta and one was juxtaposed to the peritoneum. The smooth face (SF) and rough face (RF) of each remnant were implanted turned to the vessel inner portion and one remnant was juxtaposed to the peritoneum. The animals were sacrificed in 4.5 - 8 months after surgery (75 - 109 kg). The remnants were assessed for aorta wall, fibroses formation in inner apposition and calcification related to the face turned to the vessel inner portion.</p> <p>Results</p> <p>The rough face showed a lower dilatation level compared to the face implanted in adjacent aorta. There was no difference between intensity and/or incidence of graft calcification when the superficies were compared. The bovine pericardium preserved in glutaraldehyde did not show alterations in its structure when implanted with different faces turned to the inner portion of vessel.</p> <p>Conclusion</p> <p>When turned to the inner portion of the vessel, the rough face of the remnant presented a lower dilatation in relation to the adjacent aorta and a better quality of endothelium layer and did not show a difference between intensity and/or incidence of graft calcification.</p
Proliferating cell nuclear antigen and heat shock protein 70 immunolocalization in invasive ductal breast cancer not otherwise specified
A series of 80 female patients undergoing surgery for primary breast
ductal infiltrating carcinoma not otherwise specified (NOS) was
immunohistochemically studied in order to verify any relationships
between Proliferating Cell Nulear Antigen (PCNA) immunostaining, Heat
Shock Protein 70 (HSP70) immunoreactivity, and several
clinicopathological predictors.
Positive PCNA scores (> 20% of strongly immunopositive malignant
nuclei) were observed in neoplastic cells’ nuclei in 13 tumors (16.25%)
and were intimately associated with axillary nodal involvement (p =
0.0131), relatively high tumor grades (p = 0.0016), increased tumor size
(p = 0.0312), and low or negative levels of estrogen receptors (p =
0.0323). HSP70 positive immunoexpression in malignant cells’ cytoplasm
(percentage of HSP70 immunoreactive cells > 10%) was detected in 33
samples (41.25%). It correlated significantly with presence of axillary
lymph nodal metastases (p = 0.0033) and rather poor tumor
differentiation (p = 0.0014), whereas an association of borderline
statistical significance emerged between HSP70 immunoreactivity and high
progesterone receptor status (p = 0.0637).
PCNA positive immunostaining demonstrates the tumors’ proliferative
fraction and might be used as an indicator of increased malignant
potential in breast cancer since it was associated with four adverse
prognosticators. HSP70 immunodetection is a probable marker of the
biological stress experienced by breast cancer cells, since it was
related to relatively high tumor grades. Since both proteins may
potentially predict disease outcome, their prognostic significance must
be validated by direct relation to survival. A multivariate statistical
analysis including the variables with which both proteins were
associated will reveal any possible independent prognostic value of PCNA
and HSP70 immunostaining in local, ductal invasive breast cancer NOS