Modified Whole-Mount In situ Hybridization Protocol for the Detection of Transgene Expression in Electroporated Chick Embryos

hybridization. hybridization (WISH).Here we describe a modification to the WISH protocol that is essential to prevent DNA cross-hybridization and to specifically detect transgene mRNA transcripts in electroporated embryos. Our optimized WISH procedure can be applied not only to electroporated chick embryos but also to other embryos or adult tissues that have been transfected with large amounts of reporter- or expression construct DNA

Tolerance and cross-tolerance to neurocognitive effects of THC and alcohol in heavy cannabis users

and cross-tolerance to neurocognitive effects of THC and alcohol in heavy cannabis user

Role of the EGF-CFC gene cripto in cell differentiation and embryo development.

The EGF-CFC proteins have been recently recognized as a novel family of extracellular factors required during early vertebrate development. Cripto is the founder member of the EGF-CFC family initially related to the epidermal growth factor (EGF); its expression is increased in human colon, gastric, pancreatic and lung carcinoma and in different types of both mouse and human breast carcinomas. Genetic studies in the mouse have established an essential role of cripto in the formation and correct positioning of the anterior-posterior axis. Furthermore, the absence of cripto results in a defective precardiac mesoderm, unable to differentiate into functional cardiomyocytes. Although mouse and human Cripto have been shown to activate the ras/raf/MAP kinase pathway in mammary epithelial cell lines, genetic evidence in Zebrafish has been provided for a relationship between the EGF-CFC proteins and Nodal, a member of the transforming growth factor family. Here we review the biological role of cripto in development and differentiation, pointing out recent findings on the biochemical interactions of Cripto, Nodal and Activin-like receptors

Cripto in tumors and embryo development.

The discovery of a new gene with unknown functions and encoding a protein with novel characteristics left us with the puzzling question of a suitable name for it. The name cripto [1] was chosen to indicate that the main characteristic of this novel gene was the di¤culty in ¢nding its function. Nearly 10 years passed before we started to have a clue to some of the possible functions of cripto. This review focuses on the di¡erent studies that have ¢nally provided these clues

Correlation of the transaminases alteration with the clinical severity of patients with COVID-19: A meta-analysis of the first global outbreak

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FREE LIGHT CHAINS: A NEW POTENTIAL BIOMARKER FOR DISEASE STRATIFICATION IN NON-ALCOHOLIC STEATOHEPATITIS

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FREQUENCY AND REASONS FOR NON-LISTING IN REFERRED ADULT PATIENTS FOR LIVER TRANSPLANTATION

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