Validity and reliability of the Clinical Learning Environment, Supervision and Nurse Teacher (CLES plus T), Turkish version

Aim: A methodological type of study was conducted for the purpose of investigating the validity and reliability of the Turkish version of the Clinical Learning Environment, Supervision and Nurse Teacher (CLES+T) evaluation scale of the clinical learning environment of students, clinical nurses, and educators. Methods: Sample was comprised of 602 Turkish nursing students with clinical practice experience at the hospital. The CLES+T, developed by Saarikoski, was used for data collection. Language equivalency, internal consistency, item-total correlation, and structure validity were conducted within the scope of the validity and reliability study on the CLES +T scale. Results: It was determined that item-total correlations of four items were lower than 0.30, and those items were removed from the scale as a result of item analysis. The Cronbach's alpha value of the scale was 0.93-0.99; item total point correlations of the scale varied between 0.45 and 0.66; six factors were identified in the CLES+T factor analysis study, with a total variance explained by these six factors of 64%. Conclusion: According to the findings of the research, the CLES+T Turkish version was found to be a valid and reliable scale, which can be used to evaluate satisfaction of nursing students with their clinical education in Turkey

Ultra-Fast and Sensitive Detection of Non-Typhoidal Salmonella Using Microwave-Accelerated Metal-Enhanced Fluorescence (“MAMEF”)

Certain serovars of Salmonella enterica subsp. enterica cause invasive disease (e.g., enteric fever, bacteremia, septicemia, meningitis, etc.) in humans and constitute a global public health problem. A rapid, sensitive diagnostic test is needed to allow prompt initiation of therapy in individual patients and for measuring disease burden at the population level. An innovative and promising new rapid diagnostic technique is microwave-accelerated metal-enhanced fluorescence (MAMEF). We have adapted this assay platform to detect the chromosomal oriC locus common to all Salmonella enterica subsp. enterica serovars. We have shown efficient lysis of biologically relevant concentrations of Salmonella spp. suspended in bacteriological media using microwave-induced lysis. Following lysis and DNA release, as little as 1 CFU of Salmonella in 1 ml of medium can be detected in <30 seconds. Furthermore the assay is sensitive and specific: it can detect oriC from Salmonella serovars Typhi, Paratyphi A, Paratyphi B, Paratyphi C, Typhimurium, Enteritidis and Choleraesuis but does not detect Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Streptococcus pneumoniae, Haemophilus influenzae or Acinetobacter baumanii. We have also performed preliminary experiments using a synthetic Salmonella oriC oligonucleotide suspended in whole human blood and observed rapid detection when the sample was diluted 1∶1 with PBS. These pre-clinical data encourage progress to the next step to detect Salmonella in blood (and other ordinarily sterile, clinically relevant body fluids)

Fabrication of Worm-Like Nanorods and Ultrafine Nanospheres of Silver Via Solid-State Photochemical Decomposition

Worm-like nanorods and nanospheres of silver have been synthesized by photochemical decomposition of silver oxalate in water by UV irradiation in the presence of CTAB and PVP, respectively. No external seeds have been employed for the synthesis of Ag nanorods. The synthesized Ag colloids have been characterized by UV-visible spectra, powder XRD, HRTEM, and selected area electron diffraction (SAED). Ag nanospheres of average size around 2 nm have been obtained in the presence of PVP. XRD and TEM analyses revealed that top and basal planes of nanorods are bound with {111} facets. Williamson–Hall plot has revealed the presence of defects in the Ag nanospheres and nanorods. Formation of defective Ag nanocrystals is attributed to the heating effect of UV-visible irradiation

Resisting Sleep Pressure:Impact on Resting State Functional Network Connectivity

In today's 24/7 society, sleep restriction is a common phenomenon which leads to increased levels of sleep pressure in daily life. However, the magnitude and extent of impairment of brain functioning due to increased sleep pressure is still not completely understood. Resting state network (RSN) analyses have become increasingly popular because they allow us to investigate brain activity patterns in the absence of a specific task and to identify changes under different levels of vigilance (e.g. due to increased sleep pressure). RSNs are commonly derived from BOLD fMRI signals but studies progressively also employ cerebral blood flow (CBF) signals. To investigate the impact of sleep pressure on RSNs, we examined RSNs of participants under high (19 h awake) and normal (10 h awake) sleep pressure with three imaging modalities (arterial spin labeling, BOLD, pseudo BOLD) while providing confirmation of vigilance states in most conditions. We demonstrated that CBF and pseudo BOLD signals (measured with arterial spin labeling) are suited to derive independent component analysis based RSNs. The spatial map differences of these RSNs were rather small, suggesting a strong biological substrate underlying these networks. Interestingly, increased sleep pressure, namely longer time awake, specifically changed the functional network connectivity (FNC) between RSNs. In summary, all FNCs of the default mode network with any other network or component showed increasing effects as a function of increased 'time awake'. All other FNCs became more anti-correlated with increased 'time awake'. The sensorimotor networks were the only ones who showed a within network change of FNC, namely decreased connectivity as function of 'time awake'. These specific changes of FNC could reflect both compensatory mechanisms aiming to fight sleep as well as a first reduction of consciousness while becoming drowsy. We think that the specific changes observed in functional network connectivity could imply an impairment of information transfer between the affected RSNs

Iron Behaving Badly: Inappropriate Iron Chelation as a Major Contributor to the Aetiology of Vascular and Other Progressive Inflammatory and Degenerative Diseases

The production of peroxide and superoxide is an inevitable consequence of aerobic metabolism, and while these particular "reactive oxygen species" (ROSs) can exhibit a number of biological effects, they are not of themselves excessively reactive and thus they are not especially damaging at physiological concentrations. However, their reactions with poorly liganded iron species can lead to the catalytic production of the very reactive and dangerous hydroxyl radical, which is exceptionally damaging, and a major cause of chronic inflammation. We review the considerable and wide-ranging evidence for the involvement of this combination of (su)peroxide and poorly liganded iron in a large number of physiological and indeed pathological processes and inflammatory disorders, especially those involving the progressive degradation of cellular and organismal performance. These diseases share a great many similarities and thus might be considered to have a common cause (i.e. iron-catalysed free radical and especially hydroxyl radical generation). The studies reviewed include those focused on a series of cardiovascular, metabolic and neurological diseases, where iron can be found at the sites of plaques and lesions, as well as studies showing the significance of iron to aging and longevity. The effective chelation of iron by natural or synthetic ligands is thus of major physiological (and potentially therapeutic) importance. As systems properties, we need to recognise that physiological observables have multiple molecular causes, and studying them in isolation leads to inconsistent patterns of apparent causality when it is the simultaneous combination of multiple factors that is responsible. This explains, for instance, the decidedly mixed effects of antioxidants that have been observed, etc...Comment: 159 pages, including 9 Figs and 2184 reference

Turkey and its associated factors

Background This study was performed to determine the prevalence of elder abuse among community-dwelling older adults and its associated risk factors. Methods The sample in this cross-sectional study consisted of 691 older adults. Data were collected with an elder abuse and neglect assessment form, which had been prepared by the researchers in accordance with the literature. Logistic regression analysis was used to identify factors related to subtypes of elder abuse. Results In this study, 13.6% of the elderly subjects (n = 94) were exposed to at least one instance of abusive behaviour within the last year; emotional abuse was the most frequently occurring type of abuse. Risk factors for abuse were being 75 years old or older (odds ratio (OR) = 1.993, 95% confidence interval (CI): 1.105-3.592), being illiterate (OR = 4.863, 95%CI: 2.809-8.417), lack of own income (OR = 2.189, 95%CI: 1.179-4.064), having bad family relationships (OR = 20.564, 95%CI: 5.453-77.554), and lack of own home (OR = 3.945,; 95%CI: 1.167-13.330). Conclusions The results of this study showed that abuse was an important problem for the elderly population. Therefore, elderly individuals, especially those who are 75 years old or older, have a low education level, lack their own home, and lack their own income, should be evaluated for abuse.C1 [Altintas, Hulya Kulakci] Zonguldak Bulent Ecevit Univ, Fac Hlth Sci, Dept Nursing, Zonguldak, Turkey.[Aslan, Gulbahar Korkmaz] Pamukkale Univ, Fac Hlth Sci, Dept Nursing, Denizli, Turkey

roeseli

The body length increases in Gammarus roeseli starting from different initial body lengths were measured at a range of temperatures in laboratory conditions. As a result, the growth equation of G. roeseli was calculated as: daily growth rate (DGR) = 0.0545 + 0.00143 T - 0.00172 L, where T = temperature and L = length. The mean duration of maturation was calculated as 3.5 months (15 weeks) using the DGR equation. This equation demonstrated that newly hatched larvae with a body size of 2.0 mm in May will grow to 10 mm by the middle of August, while larvae with a body size of 2.0 mm in March will grow to 10 mm by the end of June. Thus, the duration of the growth period varies throughout the year

Promotionscale

Background A measure for healthy lifestyle behaviours of older adults would enable the development of effective and target-based health promotion strategies. Aim This study aimed to test the validity and reliability of the Turkish version ofGeriatric Health Promotionscale among older people in Turkey. Design This was a methodological study that was conducted between June and August 2018. Methods A total of 321 volunteer older individuals, who were admitted to local health centres, participated in this study. A questionnaire, including sociodemographic data form, and the Geriatric Health Promotion scale were used to collect data. Content and construct validity was established using validity analyses. Results The reliability of the scale was confirmed by its internal consistency (alpha= .82) and test-retest reliability (0.86). Exploratory factor analysis for the six-factor construct explained 64.7% of the total variance. Factor loads of each item ranged from 0.44 and 0.91. Confirmatory factor analysis indicated that the theoretical constructs had a perfect fit with data obtained. These data establish that the Turkish version of the Geriatric Health Promotion scale was valid and reliable. Conclusion The Turkish version of the Geriatric Health Promotion scale is a reliable and valid instrument that can be used in the evaluation of health-promoting behaviours among older individuals.C1 [Kartal, Asiye; Aslan, Gulbahar Korkmaz; Kostu, Nazan] Pamukkale Univ, Fac Hlth Sci, Publ Hlth Nursing Dept, Pamukkale, Turkey.[Tumer, Adile] Mugla Sitki Kocman Univ, Fac Hlth Sci, Publ Hlth Nursing Dept, Mugla, Turkey