Suitability of existing Musa morphological descriptors to characterize East African highland ‘matooke’ bananas

Article purchased; Published online: 18 Sept 2017Morphological traits are commonly used for characterizing plant genetic resources. Germplasm characterization should be based on distinctly identifiable, stable and heritable traits that are expressed consistently and are easy to distinguish by the human eye. Characterization and documentation of a representative sample of East African highland bananas (Lujugira–Mutika subgroup) was carried out following an internationally accepted standard protocol for bananas. Eleven cultivars were characterized using an existing set of minimum descriptors (31 qualitative and quantitative traits) with the aim of determining stable descriptors and the ability of these descriptors to distinguish among East African highland banana cultivars. There was variation in stability of these descriptors within cultivars and across the 11 cultivars. Only 10 (32%) out of 31 descriptors studied were stable in the 11 cultivars. However, they had similar scores and therefore are not suitable to distinguish between cultivars within this group. Nonetheless, these 10 descriptors may be useful for distinguishing the East African highland bananas as a group from other groups of bananas. A few descriptors were unique to the cultivar ‘Tereza’ and may be used to distinguish this cultivar from other ‘matooke’ cultivars. None of the quantitative descriptors were stable

Innovative Partnership Approach to Chickpea seed production and Technology Dissemination: Lessons from Ethiopia

Innovative partnership in chickpea seed production and technology dissemination was assessed in increasing chickpea productivity through facilitated access to quality seed. The study was designed to evaluate the benefits gained from partnership implemented for the last 10 years in chickpea technology by seed system of crop production. Farmers' participatory variety selection (FPVS)' has been implemented as epicenter of the new innovation. The varieties selected by the farmers were ultimately demonstrated and visited by large number of farmers and other stakeholders. This was complemented with the establishment and technical backstopping of farmers' seed producer associations to produce and market quality seed to fill the gap by the formal sector. Intervention with chickpea technologies were initiated right after training partners in chickpea seed and grain vale chain. From 2008 to 2015, over 350 FPVS trials were conducted concurrently with demonstrations. The farmers' seed producer associations cover the lion's share of the seed supply and became an innovative approach that enhanced the chickpea seed system. The production of certified and quality declared seed increased from 632.7t in 2008 to 3290 tons in 2014. The drastic increase in productivity and production of chickpea during the intervention period were largely attributed to the interventions made. The national chickpea productivity has doubled in a decade's time (2006-2015) and attained peak of 1.9ton ha-1 in 2014. Similarly production showed drastic increase during the intervention period where the production in 2015 was 124% more compared to the production in 2006. Lessons were driven from the approach that many development parties and the government policy recognized the approach as key player for enhancing the chickpea sector

Genotype by environment interaction and grain yield stability of Ethiopian black seeded finger millet genotypes

Finger millet ( Eleusine coracana \ua0(L.) Gaertn) is an important cereal widely produced in Ethiopia across diverse agro-ecologies. It is valued by local farmers for its ability to grow in adverse agro-climatic conditions, where other cereals fail. The yield potential of this crop is in the range of 4-5 tonnes/ha, but the current national average grain yield is far below the potential (2.1 tonnes). Lack of improve varieties which are stable, high yielder and stress tolerant is a major limiting factor to production of this crop in Ethiopia. A field experiment was conducted using twelve black seeded finger millet (Eleusine coracana subsp. coracana) genotypes, including local and standard checks (Degu) at two locations (Bako and Gute) in Ethiopia for three years (2014 - 2016). The objective of this study was to identify stable and high yielding genotypes for grain yield and other agronomic traits among the black seeded finger millet genotypes of Ethiopia. The additive main effect and multiplicative interaction (AMMI) model analysis of variance revealed highly significant (P<0.01) differences between environments, genotype, and Interaction Principal Component Analysis (IPCA-I), but significant variations (P<0.05) for G x E interactions. This indicates that the genotypes performed differently over environments and that the test environments are highly variable. Only the first IPCA-I showed high significance (P<0.01) and contributed 48.39% of the total genotype by environment interaction (G x E). Genotypes BKFM0020, BKFM0006 and BKFM0010, which had high grain yield, but with IPCA value close to zero, indicated the wide adaptability/stability. Similarly, analysis using Eberhart and Russell model revealed that these genotypes were within the relatively acceptable range of regression coefficients (bi), approaching to one (0.742, 0.8176 and 1.0578), and deviation from regression closer to zero (s2di) (0.0385, -0.0661 and -0.0248), respectively. This implied that pipeline genotypes were stable, widely adaptable and high yielders than the other genotypes. Genotype and genotype by environment (GGE bi-plot) analysis also revealed that these candidate genotypes were stable and high yielder. Besides, these genotypes showed resistance to blast disease, which is a threat to finger millet production in the study areas. Therefore, these genotypes were selected as potential candidates for possible release in western Oromia and similar agro-ecologies of the country.Le petit mil ( Eleusine coracana (L.) Gaertn) est une c\ue9r\ue9ale importante largement produite en \uc9thiopie dans les diff\ue9rents zones agro-\ue9cologiques. Il est appr\ue9ci\ue9 par les agriculteurs locaux pour sa capacit\ue9 de grandir dans des conditions agro-climatiques d\ue9favorables, o\uf9 les autres c\ue9r\ue9ales \ue9chouent. Le potentiel du rendement en grain de cette culture de mil se situe entre 4 \ue0 5 t ha-1, mais le rendement moyen national actuel est tr\ue8s inf\ue9rieur au potentiel (2,1 tonnes). Le manque de vari\ue9t\ue9s am\ue9lior\ue9es, stables, \ue0 rendement en grain \ue9lev\ue9 et tol\ue9rantes au stress est un t facteur majeur limitant la production de cette culture de mil en \uc9thiopie. Une exp\ue9rience au champs a \ue9t\ue9 conduite avec douze g\ue9notypes des grains noir de mil (Eleusine coracana subsp. Coracana), y compris des contr\uf4les locaux et standard (Degu) \ue0 deux locations (Bako et Gute) en \uc9thiopie pendant trois ans (2014 - 2016). L\u2019objectif de cette \ue9tude \ue9tait d\u2019identifier des g\ue9notypes stables et de rendement tr\ue8s\ua0\ue9lev\ue9 pour le rendement en grain de mil et d\u2019autres caract\ue9ristiques agronomiques parmi les genotypes des grains noir de mil en \uc9thiopie. L\u2019analyse de variance du methode avec effet principal additif et interaction multiplicative (AIM) a r\ue9v\ue9l\ue9 des diff\ue9rences tr\ue8s significatives (P <0,01) entre les locations, le g\ue9notype et l\u2019analyse en composantes principales de l\u2019interaction (IPCA-I), mais des variations significatives (P <0,05) pour G x E interactions. Cela indique que les g\ue9notypes se sont comport\ue9s diff\ue9remment selon les locations et que les locations de test sont tr\ue8s variables. Seule la premi\ue8re IPCA-I a montr\ue9 une signification \ue9lev\ue9e (P <0,01) et a contribu\ue9 pour 48,39% du total des interactions g\ue9notype par la location (G x E). G\ue9notypes BKFM0020, BKFM0006 et BKFM0010, qui avait un rendement en grain \ue9lev\ue9, mais avec une valeur IPCA proche de z\ue9ro, a indiqu\ue9 la grande adaptabilit\ue9 / stabilit\ue9. De m\ueame, une analyse utilisant les m\ue9thodes\ua0d\u2019Eberhart et Russell a r\ue9v\ue9l\ue9 que ces g\ue9notypes se situaient dans la plage relativement acceptable des coefficients de r\ue9gression (bi), se rapprochant de un (0,742, 0,8176 et 1,0578) et de l\u2019\ue9cart par rapport \ue0 la r\ue9gression proche de z\ue9ro (s2di) (0,0385, 0,0661 et -0,0248), respectivement. Cela impliquait que les g\ue9notypes de pipeline \ue9taient stables, largement adaptables et ont eu le rendement tr\ue8s\ua0\ue9lev\ue9 que les autres g\ue9notypes. Les analyses de g\ue9notype et g\ue9notype par la location (bi-parcelle GGE) ont \ue9galement r\ue9v\ue9l\ue9 que ces g\ue9notypes candidats \ue9taient stables et avaient une bonne rentabilit\ue9. En outre, ces g\ue9notypes ont montr\ue9 une r\ue9sistance \ue0 la maladie fongique qui constitue une menace pour la production de mil dans les zones d\u2018 \ue9tude. Par cons\ue9quent, ces g\ue9notypes ont \ue9t\ue9 s\ue9lectionn\ue9s comme candidats potentiels pour une lib\ue9ration \ue9ventuelle dans l\u2019ouest d\u2019Oromia et des zones agro-\ue9cologiques similaires du pays

Significant progressive heterobeltiosis in banana crossbreeding

Open Access Journal; Published online: 27 Oct 2020Background Heterobeltiosis is the phenomenon when the hybrid’s performance is superior to its best performing parent. Banana (Musa spp. AAA) breeding is a tedious, time-consuming process, taking up to two decades to develop a consumer acceptable hybrid. Exploiting heterobeltiosis in banana breeding will help to select breeding material with high complementarity, thus increasing banana breeding efficiency. The aim of this study was therefore to determine and document the level of heterobeltiosis of bunch weight and plant stature in the East African highland bananas, in order to identify potential parents that can be used to produce offspring with desired bunch weight and stature after a few crosses. Results This research found significant progressive heterobeltiosis in cross-bred ‘Matooke’ (highland cooking) banana hybrids, also known as NARITAs, when grown together across years with their parents and grandparents in Uganda. Most (all except 4) NARITAs exhibited positive heterobeltiosis for bunch weight, whereas slightly more than half of them had negative heterobeltiosis for stature. The secondary triploid NARITA 17 had the highest heterobeltiosis for bunch weight: 249% versus its ‘Matooke’ grandparent and 136% against its primary tetraploid parent. Broad sense heritability (across three cropping cycles) for yield potential and bunch weight were high (0.84 and 0.76 respectively), while that of plant stature was very low (0.0035). There was a positive significant correlation (P < 0.05) between grandparent heterobeltiosis for bunch weight and genetic distance between parents (r = 0.39, P = 0.036), bunch weight (r = 0.7, P < 0.001), plant stature (r = 0.38, P = 0.033) and yield potential (r = 0.59, P < 0.001). Grandparent heterobeltiosis for plant stature was significantly, but negatively, correlated to the genetic distance between parents (r = − 0.6, P < 0.001). Conclusions Such significant heterobeltiosis exhibited for bunch weight is to our knowledge the largest among main food crops. Since bananas are vegetatively propagated, the effect of heterobeltiosis is easily fixed in the hybrids and will not be lost over time after the release and further commercialization of these hybrids

Crossbreeding east African highland bananas: lessons learnt relevant to the botany of the crop after 21 years of genetic enhancement

Open Access JournalEast African highland bananas (EAHB) were regarded as sterile. Their screening for female fertility with “Calcutta 4” as male parent revealed that 37 EAHB were fertile. This was the foundation for the establishment of the EAHB crossbreeding programs by the International Institute of Tropical Agriculture (IITA) and the National Agricultural Research Organization (NARO) in Uganda in the mid-1990s. The aim of this study was to assess the progress and efficiency of the EAHB breeding program at IITA, Sendusu in Uganda. Data on pollinations, seeds generated and germinated, plus hybrids selected between 1995 and 2015 were analyzed. Pollination success and seed germination percentages for different cross combinations were calculated. The month of pollination did not result in significantly different (P = 0.501) pollination success. Musa acuminata subsp. malaccensis accession 250 had the highest pollination success (66.8%), followed by the cultivar “Rose” (66.6%) among the diploid males. Twenty-five EAHB out of 41 studied for female fertility produced up to 305 seeds per pollinated bunch, and were therefore deemed fertile. The percentage of seed germination varied among crosses: 26% for 2x × 4x, 23% for 2x × 2x, 11% for 3x × 2x, and 7% for 4x × 2x. Twenty-seven NARITA hybrids (mostly secondary triploids ensuing from the 4x × 2x) were selected for further evaluation in the East African region. One so far –“NARITA 7”– was officially released to farmers in Uganda. Although pollination of EAHB can be conducted throughout the year, the seed set and germination is low. Thus, further research on pollination conditions and optimization of embryo culture protocols should be done to boost seed set and embryo germination, respectively. More research in floral biology and seed germination as well as other breeding strategies are required to increase the efficiency of the EAHB breeding program

Prevalence of depression and anxiety symptoms and their determinant factors among patients with cancer in southern Ethiopia: a cross-sectional study

ObjectiveThe study was aimed to assess the prevalence of depression and anxiety symptoms and their determinant factors among patients with cancer attending follow-up at Hawassa University Comprehensive Specialized Hospital cancer treatment centre, Ethiopia.DesignInstitution-based cross-sectional study design was implemented.SettingPatients with cancer at Hawassa University Comprehensive Specialized Hospital cancer treatment centre from October 2019 to December 2019.ParticipantsRandomly selected 415 patients with cancer who had follow-up at cancer treatment centre.Main outcome measuresAnxiety and depression symptoms were assessed using Hospital Anxiety and Depression Scale.ResultThe prevalence rates of depression and anxiety symptoms were found to be 244 (58.8%) and 249 (60.0%), respectively. Older age (&gt;50 years) (AOR (adjusted OR)=2.24, 95% CI=1.14 to 4.40), being unemployed (AOR=1.96, 95% CI=1.08 to 3.56), advanced stage of cancer such as stage III (AOR=5.37, 95% CI=1.34 to 21.45) and stage IV (AOR=4.55, 95% CI=1.12 to 18.44), comorbid psychotic symptoms (AOR=1.67, 95% CI=1.07 to 2.61) and eating problem in the past 2 weeks (AOR=6.16, 95% CI=1.98 to 19.11) were independent factors significantly associated with depressive symptoms. In addition, cancer stage such as stage II (AOR=3.92, 95% CI=1.07 to 14.36) and stage IV (AOR=5.04, 95% CI=1.44 to 17.59) and comorbid psychotic symptoms (AOR=1.73, 95% CI=1.12 to 2.66) were significantly associated with anxiety symptoms.ConclusionDepression and anxiety symptoms among patients with cancer were considerably high. Age, occupation, cancer stage, comorbid psychotic symptoms and eating problem were determinant factors of depressive symptoms among patients with cancer. Moreover, cancer stage and comorbid psychosis were determinants of anxiety symptoms. Healthcare professionals working in the oncology unity need to conduct routine screening and treatment of depression and anxiety symptoms for patients with cancer.</jats:sec

A Prospective Study of Causes of Illness and Death in Preterm Infants in Ethiopia: The SIP Study Protocol

Background With nearly 15 million annual preterm births globally, preterm birth is the most common cause of neonatal death. Forty to 60 % of neonatal deaths are directly or indirectly associated with preterm mortality. As countries aim to meet the Sustainable Development Goals to reduce neonatal mortality, significant reductions in preterm mortality are needed. This study aims to identify the common causes of preterm illness and their contribution to preterm mortality in low-resource settings. This article will describe the methods used to undertake the study. Methods This is a prospective, multi-centre, descriptive clinical study. Socio-demographic, obstetric, and maternal factors, and clinical and laboratory findings will be documented. The major causes of preterm mortality will be identified using clinical, laboratory, imaging, and autopsy methods and use the national Ethiopian guidelines on management of preterm infants including required investigations to reach final diagnoses. The study will document the clinical and management protocols followed in these settings. The approach consists of clinical examinations and monitoring, laboratory investigations, and determination of primary and contributory causes of mortality through both clinical means and by post-mortem examinations. An independent panel of experts will validate the primary and contributory causes of mortality. To obtain the estimated sample size of 5000 preterm births, the study will be undertaken in five hospitals in three regions of Ethiopia, which are geographically distributed across the country. All preterm infants who are either born or transferred to these hospitals will be eligible for the study. Three methods (last menstrual period, physical examination using the New Ballard Score, and ultrasound) will be used to determine gestational age. All clinical procedures will be conducted per hospital protocol and informed consent will be taken from parents or caretakers prior to their participation in the study as well as for autopsy if the infant dies. Discussion This study will determine the major causes of death and illness among hospitalized preterm infants in a low-resource setting. The result will inform policy makers and implementers of areas that can be prioritized in order to contribute to a significant reduction in neonatal mortality