Regulations of ferritin gene expression in plants

International audienc

When iron meets light : regulation of iron homesotasis by light, circadian clock and oxidative stress

When iron meets light : regulation of iron homesotasis by light, circadian clock and oxidative stress. 4th Umea Plant Science Center-INRA bilateral meetin

Diversité des profils d’activité des chercheurs INRAE

INRAE fait évaluer ses chercheurs tous les 2-3 ans par des commissions d’évaluation sur quatre grandes dimensions des missions de chercheur : la production de connaissances, l’expertise, la formation et le management. Les chercheurs doivent répartir les pourcentages de temps qu’ils passent à ces quatre différents types de mission, constituant ainsi un profil d’activité. L’objectif de ce travail est d’analyser les profils d’activité déclarés par chercheurs INRAE sur les sept dernières années d’évaluation, en créant une typologie des profils de chercheurs par grade par une classification hiérarchique. Cette analyse montre une très grande diversité des profils d’activité, allant de profils majoritairement dans la production de connaissances à d’autres plus centrés sur l’animation et la direction de collectifs. Une constante peut être notée : tous les profils présentent une part de production de connaissances, quel que soit le grade du chercheur.En conclusion, il existe une diversité importante des profils des chercheurs et chercheuses INRAE, indiquant qu’il existe de multiples façon d’exercer le métier de chercheur dans cet institut de recherche finalisée

The Transcriptional Control of Iron Homeostasis in Plants: A Tale of bHLH Transcription Factors?

Iron is one of the most important micronutrients in plants as it is involved in many cellular functions (e.g., photosynthesis and respiration). Any defect in iron availability will affect plant growth and development as well as crop yield and plant product quality. Thus, iron homeostasis must be tightly controlled in order to ensure optimal absorption of this mineral element. Understanding mechanisms governing iron homeostasis in plants has been the focus of several studies during the past 10 years. These studies have greatly improved our understanding of the mechanisms involved, revealing a sophisticated iron-dependent transcriptional regulatory network. Strikingly, these studies have also highlighted that this regulatory web relies on the activity of numerous transcriptional regulators that belong to the same group of transcription factors (TF), the bHLH (basic helix-loop-helix) family. This is best exemplified in Arabidopsis where, to date, 16 bHLH TF have been characterized as involved in this process and acting in a complex regulatory cascade. Interestingly, among these bHLH TF some form specific clades, indicating that peculiar function dedicated to the maintenance of iron homeostasis, have emerged during the course of the evolution of the green lineage. Within this mini review, we present new insights on the control of iron homeostasis and the involvement of bHLH TF in this metabolic proces

Iron and ferritin dependent ROS distribution impact Arabidopsis root

Iron and ferritin dependent ROS distribution impact Arabidopsis root. XVII Iron Symposium on Iron Nutrition and Interactions in Plant

Twenty five years of ferritin research : what's up now ?

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Roles and maturation of iron–sulfur proteins in plastids

One reason why iron is an essential element for most organisms is its presence in prosthetic groups such as hemes or iron–sulfur (Fe–S) clusters, which are notably required for electron transfer reactions. As an organelle with an intense metabolism in plants, chloroplast relies on many Fe–S proteins. This includes those present in the electron transfer chain which will be, in fact, essential for most other metabolic processes occurring in chloroplasts, e.g., carbon fixation, nitrogen and sulfur assimilation, pigment, amino acid, and vitamin biosynthetic pathways to cite only a few examples. The maturation of these Fe–S proteins requires a complex and specific machinery named SUF (sulfur mobilisation). The assembly process can be split in two major steps, (1) the de novo assembly on scaffold proteins which requires ATP, iron and sulfur atoms, electrons, and thus the concerted action of several proteins forming early acting assembly complexes, and (2) the transfer of the preformed Fe–S cluster to client proteins using a set of late-acting maturation factors. Similar machineries, having in common these basic principles, are present in the cytosol and in mitochondria. This review focuses on the currently known molecular details concerning the assembly and roles of Fe–S proteins in plastids

Subcellular localization of ferritin mRNA in Arabidopsis thaliana mutants impaired in mRNA decay

Subcellular localization of ferritin mRNA in Arabidopsis thaliana mutants impaired in mRNA decay. 3e journées scientifiques et techniques du réseau des microscopistes Inra : " De l'imagerie multiple à l'imagerie multimodale

Iron-induced release of intracellular free calcium is involved in growth arrest of Arabidopsis thaliana primary root

Iron-induced release of intracellular free calcium is involved in growth arrest of Arabidopsis thaliana primary root. 11th national meeting of the Société Française de Biologie Végétale SFB

Spatio-Temporal Imaging of Promoter Activity in Intact Plant Tissues

Localization and quantification of expression levels of genes help to determine their function. Localization of gene expression is often achieved through the study of their promoter activity. Three main reporter genes beta-glucuronidase (GUS), green fluorescent protein (GFP), and luciferase (LUC) have been intensively used to characterize promoter activities, each having its own specificities and advantages. Among them, the LUC reporter gene is best suitable for the analysis of the promoter activity of genes in intact living plants. Here, we describe a LUC-based method that allows to precisely localize and quantify promoter activity at the whole plant level, and to study the mechanisms that are involved in long-distance regulation of gene expression in Arabidopsis thaliana. Imaging LUC signals with a low-light CCD camera allows monitoring promoter activity in time and space in the transgenic plant harboring the promoter fused with the LUC gene. In addition, it allows quantifying change of promoter activities in plant during several hours