Physicochemical and sensory analysis of instant cereal beverage incorporated with corncob powder

The primary objectives of this study were to process corncob into corncob powder (CCP) and to apply CCP in the formulation of instant cereal beverage (ICB) in order to produce high fibre ICB, and to investigate the physicochemical and sensory properties of the corncob-based instant cereal beverage. Corncobs were sourced and washed thoroughly before drying and grinding into CCP. CCP was then imparted into ICB formulation in three different ratios (10, 20 and 30% w/w) to partially substitute corn flour in the formulation. All four ICB samples including the commercial counterpart were analysed for their physicochemical and sensory properties. The incorporation of CCP has affected the viscosity, colour and sensory attributes significantly of the produced ICB. Higher contents of CCP in the formulation was found to be responsible for less viscous and browner effect compared to the commercial ICB samples. Formulation of ICB incorporated with 30% w/w CCP had the highest mean scores (6.00, p<0.05) of overall acceptability among all the other formulations and it was comparable to the commercial ICB in the current market

High intrinsic biosorption efficiency of cattle manure on Cr(VI): a potential low-cost fibre-rich biosorbent

Fibre-rich manure derived from grass-fed cattle showed significantly higher intrinsic sorption efficiency on Cr(VI) solution as compared to corncob, sawdust and cogon grass. This observation could be attributed to the ligneous nature and rough surface morphology of the cattle manure. Four-factor, three-level, face-centred composite design (FCCD) suggested the process was greatly affected by initial pH of the solution, contact time and sorbent dosage (p50% adsorption efficiency. It is predicted that both physisorption and chemisorption are involved in the sorption process

Physicochemical and sensory analyses of high fibre bread incorporated with corncob powder

The primary objectives of the present work were to produce corncob powder (CCP) from corncobs and incorporate the CCP into bread formulation in order to develop high fibre bread, and to investigate the physicochemical and sensory properties of the produced high fibre bread (HFB). The corncobs were collected and washed before they underwent the grinding and drying processes. The obtained CCP was incorporated into the bread formulation in three different proportions (5, 10 and 20%) to partially substitute bread flour in the formulation. All three bread samples and the control (0% CCP in the formulation) were analysed to obtain their physicochemical and sensory properties. The incorporation of CCP significantly affected the texture, colour and volume attributes of the produced breads. Increasing the content of CCP in the formulation was found to be responsible for firmer, smaller and darker bread loaves as compared to the composite bread samples. The bread formulation incorporated with 10% CCP had the highest mean scores (7.00) of overall acceptability among all the other formulations, and it was comparable to the commercial breads in the current market

Distinct routes of lineage development reshape the human blood hierarchy across ontogeny.

In a classical view of hematopoiesis, the various blood cell lineages arise via a hierarchical scheme starting with multipotent stem cells that become increasingly restricted in their differentiation potential through oligopotent and then unipotent progenitors. We developed a cell-sorting scheme to resolve myeloid (My), erythroid (Er), and megakaryocytic (Mk) fates from single CD34(+) cells and then mapped the progenitor hierarchy across human development. Fetal liver contained large numbers of distinct oligopotent progenitors with intermingled My, Er, and Mk fates. However, few oligopotent progenitor intermediates were present in the adult bone marrow. Instead, only two progenitor classes predominate, multipotent and unipotent, with Er-Mk lineages emerging from multipotent cells. The developmental shift to an adult "two-tier" hierarchy challenges current dogma and provides a revised framework to understand normal and disease states of human hematopoiesis.This work was supported by Postdoctoral Fellowship Awards from Canadian Institute of Health Research (CIHR) to FN and SZ. SZ is supported by (Aplastic Anemia). FN is a recipient of a scholar’s research award from the Ontario Institute of Cancer Research (OICR), through generous support from the Ontario Ministry of Research and Innovation. Research in EL laboratory is supported by a Wellcome Trust Sir Henry Dale Fellowship and core support grant from the Wellcome Trust and MRC to the Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute. Work in the Dick laboratory is supported by grants from the CIHR, Canadian Cancer Society, Terry Fox Foundation, Genome Canada through the Ontario Genomics Institute, OICR with funds from the province of Ontario, a Canada Research Chair and the Ontario Ministry of Health and Long Term Care (OMOHLTC).This is the author accepted manuscript. The final version is available from AAAS via http://dx.doi.org/10.1126/science.aab211

Safety assessment of tocotrienol supplementation in subjects with metabolic syndrome: a randomised control trial

Previous studies have reported that tocotrienols (T3) possess many distinct properties such as antioxidant, cardioprotective, neuroprotective, anti-cancer, anti-inflammatory and anti-angiogenic, which are beneficial for the improvement of human health. However, there is limited data available on the safety assessment of T3 compared to tocopherols (T). A randomised, double-blinded, cross-over and placebo-controlled human clinical trial was conducted to determine the safety and tolerance of T3 supplementation in 31 subjects with metabolic syndrome. The subjects were supplemented with tocotrienol-rich fraction (TRF) 200 mg or placebo capsules twice daily for two weeks followed by a post-intervention visit. Results showed that T3 supplementation had no significant adverse effect on the red blood cell (RBC), white blood cell (WBC) and platelet counts between TRF (5.10 ± 0.78 × 1012 litre-1, 7.35 ± 1.59 × 109 litre-1, 279.45 ± 73.86 × 109 litre-1, respectively) and placebo interventions (5.13 ± 0.76 × 1012 litre-1, 7.25 ± 1.95 × 109 litre-1, 267.45 ± 68.72 × 109 litre-1, respectively). Measures of serum aspartate aminotransferase (AST), serum alanine aminotransferase (ALT)) and albumin did not differ between TRF (25.68 ± 10.72 IU litre-1, 38.26 ± 24.74 IU litre-1, 43.61 ± 2.26 g litre-1, respectively) and placebo interventions (27.39 ± 16.44 IU litre-1, 42.23 ± 33.58 IU litre-1, 43.68 ± 2.15 g litre-1, respectively). This study indicated that supplementation with T3 at the dosage of 400 mg per day for 14 days did not induce haematoxicity and hepatotoxicity in subjects with metabolic syndrome

BIODEGRADATION OF SYNTHETIC BIPHASIC CALCIUM-PHOSPHATE AND BIOLOGICAL CALCIFIED SUBSTRATUM BY CELLS OF HEMATOPOIETIC ORIGIN

Different types of osteoclastic cells (authentic osteoclasts from human giant cell tumor and bone marrow of newborn rats; newly-formed osteoclasts from adult rat bone marrow), giant multinucleated cells and macrophages were studied for their effect on synthetic and natural mineralized substrata. Biphasic calcium phosphate ceramic consisted of hydroxyapatite and beta tricalcium phosphate was chosen for in vitro experiments, and dentine served as a positive control for cell-resorbing activity. Our results show the limited capacity of authentic and newly-formed osteoclasts to resorb synthetic ceramic as compared to that of natural substrata. In vitro cell-mediated biodegradation included also modifications of the synthetic substratum surface caused presumably by phagocytosis of the material

BIODEGRADATION OF SYNTHETIC BIPHASIC CALCIUM-PHOSPHATE AND BIOLOGICAL CALCIFIED SUBSTRATUM BY CELLS OF HEMATOPOIETIC ORIGIN

Different types of osteoclastic cells (authentic osteoclasts from human giant cell tumor and bone marrow of newborn rats; newly-formed osteoclasts from adult rat bone marrow), giant multinucleated cells and macrophages were studied for their effect on synthetic and natural mineralized substrata. Biphasic calcium phosphate ceramic consisted of hydroxyapatite and beta tricalcium phosphate was chosen for in vitro experiments, and dentine served as a positive control for cell-resorbing activity. Our results show the limited capacity of authentic and newly-formed osteoclasts to resorb synthetic ceramic as compared to that of natural substrata. In vitro cell-mediated biodegradation included also modifications of the synthetic substratum surface caused presumably by phagocytosis of the material

Development of a liver-specific Tet-off AAV8 vector for improved safety of insulin gene therapy for diabetes

10.1002/jgm.3067JOURNAL OF GENE MEDICINE21

Update of humanized animal disease models in studying Graft- versus

Graft-versus-host disease (GVHD) is a severe adverse effect that results from bone marrow or peripheral blood cells transplantation and has a high rate of mortality. About 50% of the patients are accompanied with acute Graft-versus-Host Disease (aGVHD) after bone marrow cell transplantation and need systematic treatment. It has an important clinical significance to evaluate the prevention and treatment effects of GVHD. The stable and reliable approaches of humanized animal models are crucial for advancing on the study the biology of GVHD. Relative models transplanting the human immune cells into the mouse body can trigger immunoreaction similar to the humans. As it is a disease triggered by human immune cells, any intervention research prior to clinical treatment has more clinical interrelations compared with the general animal models. In this review, we update the current understanding on humanized animal disease models on studying Graft-versus-host disease and expect to provide more theoretical basis to further study on Graft-versus-host disease