Exploring potential germline associated roles of the TRIM-NHL protein NHL-2 through RNAi screening

TRIM-NHL proteins are highly conserved regulators of developmental pathways in vertebrates and invertebrates. The TRIM-NHL family member NHL-2 in Caenorhabditis elegans functions as a miRNA cofactor to regulate developmental timing. Similar regulatory roles have been reported in other model systems, with the mammalian ortholog in mice, TRIM32, contributing to muscle and neuronal cell proliferation via miRNA activity. Given the interest associated with TRIM-NHL family proteins, we aimed to further investigate the role of NHL-2 in C. elegans development by using a synthetic RNAi screening approach. Using the ORFeome library, we knocked down 11,942 genes in wild-type animals and nhl-2 null mutants. In total, we identified 42 genes that produced strong reproductive synthetic phenotypes when knocked down in nhl-2 null mutants, with little or no change when knocked down in wild-type animals. These included genes associated with transcriptional processes, chromosomal integrity, and key cofactors of the germline small 22G RNA pathway.Gregory M. Davis, Wai Y. Low, Joshua W.T. Anderson and Peter R. Boa

The Influence of Alfalfa Coumestrol on the Reproductive Performance of Gilts

In Australia during the early 1940\u27sa syndrome known as clover disease was observed in sheep grazing subterranean clover. This syndrome was characterized by a marked reduction in fertility which was later proved to be due to a high content of estrogenic substances in the clover. Alfalfa has since been shown to contain varying levels of these plant estrogens. The most important of the plant estrogens present in alfalfa is coumestrol because of its relatively greater biological potency than the other plant estrogens and because of its more frequent occurrence. Plant physiologists have shown that alfalfa infected with certain fungus diseases contains a level of coumestrol which increases with the amount of disease present in the alfalfa plants. Since alfalfa meal is such an important source of nutrients for farm animals and is often included in swine rations this study was conducted to determine if alfalfa plants with coumestrol levels in excess of 100 parts per million (ppm) would have any effect on the reproductive performance of gilts when included in their ration

Argon annealing of the oxygen-isotope exchanged manganite La_{0.8}Ca_{0.2}MnO_{3+y}

We have resolved a controversial issue concerning the oxygen-isotope shift of the ferromagnetic transition temperature T_{C} in the manganite La_{0.8}Ca_{0.2}MnO_{3+y}. We show that the giant oxygen-isotope shift of T_C observed in the normal oxygen-isotope exchanged samples is indeed intrinsic, while a much smaller shift observed in the argon annealed samples is an artifact. The argon annealing causes the 18O sample to partially exchange back to the 16O isotope due to a small 16O contamination in the Ar gas. Such a contamination is commonly caused by the oxygen outgas that is trapped in the tubes, connectors and valves. The present results thus umambiguously demonstrate that the observed large oxygen isotope effect is an intrinsic property of manganites, and places an important constraint on the basic physics of these materials.Comment: 4 pages, 3 figures, submitted to PR

Spectral Degeneracies in the Totally Asymmetric Exclusion Process

We study the spectrum of the Markov matrix of the totally asymmetric exclusion process (TASEP) on a one-dimensional periodic lattice at ARBITRARY filling. Although the system does not possess obvious symmetries except translation invariance, the spectrum presents many multiplets with degeneracies of high order. This behaviour is explained by a hidden symmetry property of the Bethe Ansatz. Combinatorial formulae for the orders of degeneracy and the corresponding number of multiplets are derived and compared with numerical results obtained from exact diagonalisation of small size systems. This unexpected structure of the TASEP spectrum suggests the existence of an underlying large invariance group. Keywords: ASEP, Markov matrix, Bethe Ansatz, Symmetries.Comment: 19 pages, 1 figur

Implicit discrimination of basic facial expressions of positive/negative emotion in Fragile X Syndrome and Autism Spectrum Disorder

Fragile X syndrome (FXS) and autism spectrum disorders (ASD) are characterized by impaired social functioning. We examined the spontaneous discrimination of happy and disgusted facial expressions, from neutral faces, in individuals with FXS (n  =  13, Mage  =  19.70) and ASD (n  =  15, Mage  =  11.00) matched on adaptive behavior and verbal abilities measured by the Vineland Adaptive Behavior Scale. Eye gaze to the eyes and mouth of neutral faces was also measured. Results suggest individuals with FXS and ASD distinguish facial expressions spontaneously in the same way. Individuals with FXS looked significantly less at the eye region of neutral faces than individuals with ASD. These results provide insight into similarities and differences in face processing in two neurodevelopmental disorders noted for their similarities in social behavior

Topological representations of matroid maps

The Topological Representation Theorem for (oriented) matroids states that every (oriented) matroid can be realized as the intersection lattice of an arrangement of codimension one homotopy spheres on a homotopy sphere. In this paper, we use a construction of Engstr\"om to show that structure-preserving maps between matroids induce topological mappings between their representations; a result previously known only in the oriented case. Specifically, we show that weak maps induce continuous maps and that the process is a functor from the category of matroids with weak maps to the homotopy category of topological spaces. We also give a new and conceptual proof of a result regarding the Whitney numbers of the first kind of a matroid.Comment: Final version, 21 pages, 8 figures; Journal of Algebraic Combinatorics, 201

Laser-induced collective excitations in a two-component Fermi gas

We consider the linear density response of a two-component (superfluid) Fermi gas of atoms when the perturbation is caused by laser light. We show that various types of laser excitation schemes can be transformed into linear density perturbations, however, a Bragg spectroscopy scheme is needed for transferring energy and momentum into a collective mode. This makes other types of laser probing schemes insensitive for collective excitations and therefore well suited for the detection of the superfluid order parameter. We show that for the special case when laser light is coupled between the two components of the Fermi gas, density response is always absent in a homogeneous system.Comment: 6 pages, no figure

Towards the Thermodynamics of Localization Processes

We study the entropy time evolution of a quantum mechanical model, which is frequently used as a prototype for Anderson's localization. Recently Latora and Baranger [V. Latora, M. Baranger, Phys. Rev.Lett. 82, 520(1999)] found that there exist three entropy regimes, a transient regime of passage from dynamics to thermodynamics, a linear in time regime of entropy increase, namely a thermodynamic regime of Kolmogorov kind, and a saturation regime. We use the non-extensive entropic indicator recently advocated by Tsallis [ C. Tsallis, J. Stat. Phys. 52, 479 (1988)] with a mobile entropic index q, and we find that with the adoption of the ``magic'' value q = Q = 1/2 the Kolmogorov regime becomes more extended and more distinct than with the traditional entropic index q = 1. We adopt a two-site model to explain these properties by means of an analytical treatment and we argue that Q =1/2 might be a typical signature of the occurrence of Anderson's localization.Comment: 13 pages, 8 figures submitted to Phys. Rev.

Condensation energy in strongly coupled superconductors

We consider the condensation energy in superconductors where the pairing is electronic in origin and is mediated by a collective bosonic mode. We use magnetically-mediated superconductivity as an example, and show that for large spin-fermion couplings, the physics is qualitatively different from the BCS theory as the condensation energy results from the feedback on spin excitations, while the electronic contribution to the condensation energy is positive due to an ``undressing'' feedback on the fermions. The same feedback effect accounts for the gain of the kinetic energy at strong couplings.Comment: 4 pages, revtex 4, 3 eps figure

The Practical Application of Space Nuclear Power in the 1960’s

Nuclear reactor space electric power units under development in the U. S. include the SNAP-2, SNAP-8, and SNAP-10. The electric power output of these Systems for Nuclear Auxiliary Power (SNAP) extends over three decades of power, from 300 watts to 6O kilowatts. The major operational, installation, and handling characteristics of these nuclear power units are described. In particular, some limitations and restrictions with regard to payload, shielding, and radiation environment are described with respect to the power plants, their mode of installation, and system weight. The ground handling and safety, as well as the over-all safety, aspects of space reactor utilization are also described. The SNAP-10 power unit is a demonstration system that utilizes thermoelectric power conversion, The SNAP-2 power system utilizes a similar compact nuclear reactor, which is cooled with liquid sodium-potassium alloy, and coupled to a small mercury vapor turbine generator power conversion system. The SNAP-8 system is a direct outgrowth of the SNAP-2 power plant development. It wiil deliver 30,000 watts with one mercury vapor turbine generator system and weighs about 1400 (auth