Effect of captopril on serum lipid levels and cardiac mitochondrial oxygen consumption in experimentally-induced hypercholesterolemia in rabbits

Angiotensin converting enzyme inhibitors are widely used in therapy of cardiovascular diseases. However, the consensus on effects of these inhibitors in control of myocardial oxygen consumption during the process of experimental hypercholesterolemia and under the condition of endothelial dysfunction has not been reached. Here we examined effects of captopril, an angiotensin converting enzyme inhibitor, on serum lipid levels and oxygen consumption rate in mitochondria isolated from heart of rabbits treated by hypercholesterolemic diet. During the twelve-week period, the Chinchilla male rabbits were daily treated by saline (controls); 1 % cholesterol diet; 5 mg/kg/day captopril or 1 % cholesterol + 5 mg/kg/day captopril. Total- and high-density lipoprotein cholesterol and triglyceride in serum were measured spectrophotometricly. The left ventricle mitochondrial fraction was isolated and myocardial oxygen consumption was measured by Biological Oxygen Monitor. Mitochondria isolated from hearts of rabbits exposed to hypercholesterolemic diet showed significantly reduced respiration rates (state 3 and state 4) with altering adenosine diphosphate/oxygen ratio, whereas the respiratory control ratio was not affected when compared to controls. Mitochondria from cholesterol/captopril-treated animals showed significantly reduced respiration rates without altering adenosine diphosphate/oxygen ratio index or respiratory control ratio. Although captopril did not exert the favorable effect on serum lipid levels in cholesterol-treated animals, it restored the mitochondrial oxygen consumption. Further studies should be performed to define the underlying physiological and/or pathophysiological mechanisms and clinical implications

Craniovertebral anomalies associated with pituitary gland duplication

Background: An extremely rare occurrence of the pituitary gland duplication inspired us to examine in detail the accompanying craniovertebral congenital anomalies in a patient involved. Materials and methods: T1-wighted magnetic resonance imaging (MRI) was performed, as well as the multislice computerised tomography (MSCT) and MSCT angiography in our patient, as well as in a control group of 10 healthy subjects. Results: In a 20-year-old male a double pituitary gland was identified, as well as hypothalamic enlargement, tuberomamillary fusion and hamartoma. In addition, the patient also showed a duplicated hypophyseal fossa and posterior clinoid processes, notch of the upper sphenoid, prominent inner relief of the skull, inverse shape of the foramen magnum, third occipital condyle, partial aplasia of the anterior and posterior arches of the atlas with a left arcuate foramen, duplication of the odontoid process and the C2 body, and fusion of the C2–C4 and T12–L1 vertebrae. The MSCT angiography presented a segmental dilatation of both vertebral arteries and the A2 segment of the anterior cerebral artery, as well as a duplication of the basilar artery. Conclusions: This patient is unique due to complex craniovertebral congenital anomalies associated with a duplication of the pituitary gland

Computing generalized inverses using LU factorization of matrix product

An algorithm for computing {2, 3}, {2, 4}, {1, 2, 3}, {1, 2, 4} -inverses and the Moore-Penrose inverse of a given rational matrix A is established. Classes A(2, 3)s and A(2, 4)s are characterized in terms of matrix products (R*A)+R* and T*(AT*)+, where R and T are rational matrices with appropriate dimensions and corresponding rank. The proposed algorithm is based on these general representations and the Cholesky factorization of symmetric positive matrices. The algorithm is implemented in programming languages MATHEMATICA and DELPHI, and illustrated via examples. Numerical results of the algorithm, corresponding to the Moore-Penrose inverse, are compared with corresponding results obtained by several known methods for computing the Moore-Penrose inverse

Supplementary data for the article: Savić, B. G.; Stanković, D. M.; Živković, S. M.; Ognjanović, M. R.; Tasić, G. S.; Mihajlović, I. J.; Brdarić, T. P. Electrochemical Oxidation of a Complex Mixture of Phenolic Compounds in the Base Media Using PbO2-GNRs Anodes. Applied Surface Science 2020, 529, 147120. https://doi.org/10.1016/j.apsusc.2020.147120

Supplementary material for: [https://doi.org/10.1016/j.apsusc.2020.147120]Related to published version: [https://cherry.chem.bg.ac.rs/handle/123456789/4177

The effects of mineral adsorbents added to broilers diet on breast meat quality

The aim of these investigations was to determine the influence of mineral adsorbents “Minazel” and “Minazel Plus” added into broiler diet, on the carcass quality and nutritional, technological and sensory properties of breast meat. The examination was done on Cobb 500 provenience divided into 4 groups: control group C (fed without addition of mineral adsorbent), experimental group E I (0.5% of Minazel), experimental group E II (0.2% of Minazel Plus), experimental group E III (0.3% of Minazel Plus). The results showed that the broilers fed with the addition of mineral adsorbents, had a higher (P < 0.01) mass of chilled carcass “ready to grill“ and breast mass, than the broilers of the control group. Based on the parameters and criteria for defining the quality of chicken breast meat (pHu and L*) it can be concluded that meat of all groups had in average "normal" quality. According to the results of sensory analyzed roasted breast meat, meat of experimental groups had preferable smell and tenderness. [Projekat Ministarstva nauke Republike Srbije, br. 46012

Oral cavity protozoa relevant in the practice of dentistry

Introduction: Oral cavity is the colonization site of most diverse microorganisms. The homeostasis of oral microbioma is affected by numerous factors. Periodontal diseases occur as a consequence of disturbed oral microbioma homeostasis, when an inflammatory reaction occurs in the periodontal tissue. The impact of parasites on periodontal pathophysiology has not been sufficiently studied, and present and future research should hopefully answer quite a few questions concerning the issue. Aim of the paper: In the light of the present knowledge of the pathogenesis, diagnosis and epidemiology of oral cavity infections caused by Entamoeba gingivalis and Trichomonas tenax, the aim of the paper was review of literature which could to point to the importance of protozoa in the practice of dentistry and to possible oral cavity manifestations of parasitic infections relevant for public health. Conclusion: Dentists have an essential role in the diagnosis of oral diseases caused by oral cavity protozoa, and protozoa relevant for public health that produce systemic infections, the pathological changes of which may manifest in the oral cavity. Their identification represents a challenge and requires multidisciplinary approach for a timely diagnosis and adequate management

Computation of generalized inverses using Php/MySql environment

The main aim of this paper is to develop a client/server-based model for computing the weighted Moore-Penrose inverse using the partitioning method as well as for storage of generated results. The web application is developed in the PHP/MySQL environment. The source code is open and free for testing by using a web browser. Influence of different matrix representations and storage systems on the computational time is investigated. The CPU time for searching the previously stored pseudo-inverses is compared with the CPU time spent for new computation of the same inverses.Comment: International Journal of Computer Mathematics, Volume 88, Issue 11, 201

Monte Carlo simulation as an alternative approach for estimation of uncertainty measurement of 2,4 dimethylphenol

The estimation of uncertainty measurement using different approaches is increasingly applied to assess the reliability of results generated by applied analytical methods. This paper presents the Monte Carlo simulation model (MCS) for calculating the uncertainty of the measurement associated with the result of the analysis. The results of MCS were compared with the commonly used a standard method (GUM). The calculations of the measurement uncertainty were demonstrated in the case of the determination of 2,4-dimethylphenol by gas chromatography in concrete samples.14th international conference on fundamental and applied aspects of physical chemistry; 24-28 September 2018, Belgrade, Serbia

A method for finding new sets of axioms for classes of semigroups

We introduce a general technique for finding sets of axioms for a given class of semigroups. To illustrate the technique, we provide new sets of defining axioms for groups of exponent n, bands, and semilattices