Association of Chorioamnionitis with Aberrant Neonatal Gut Colonization and Adverse Clinical Outcomes.

ObjectiveChorioamnionitis (inflammation of the placenta and fetal membranes) and abnormal gastrointestinal colonization have been associated with an increased risk of sepsis and death in preterm infants, but whether chorioamnionitis causes abnormal pioneering gastrointestinal colonization in infants is not known. We determined the relationship between chorioamnionitis, altered infant fecal microbiome indicating abnormal gastrointestinal colonization, and adverse outcomes.Study designPreterm infants ≤ 28 weeks at birth were enrolled from 3 level III NICUs in Cincinnati, Ohio and Birmingham, Alabama. Sequencing for 16S microbial gene was performed on stool samples in the first 3 weeks of life. Chorioamnionitis was diagnosed by placental histology. Late onset sepsis and death outcomes were analyzed in relation to fecal microbiota and chorioamnionitis with or without funisitis (inflammation of the umbilical cord).ResultsOf the 106 enrolled infants, 48 infants had no chorioamnionitis, 32 infants had chorioamnionitis but no funisitis (AC), and 26 infants had chorioamnionitis with funisitis (ACF). The fecal samples from ACF infants collected by day of life 7 had higher relative abundance of family Mycoplasmataceae (phylum Tenericutes), genus Prevotella (phylum Bacteroidetes) and genus Sneathia (phylum Fusobacteria). Further, AC and ACF infants had higher incidence of late-onset sepsis/death as a combined outcome. Presence of specific clades in fecal samples, specifically, order Fusobacteria, genus Sneathia or family Mycoplasmataceae, were significantly associated with higher risk of sepsis or death.ConclusionThe results support the hypothesis that specific alterations in the pioneering infant gastrointestinal microbiota induced by chorioamnionitis predispose to neonatal sepsis or death

Classification of All Poisson-Lie Structures on an Infinite-Dimensional Jet Group

A local classification of all Poisson-Lie structures on an infinite-dimensional group $G_{\infty}$ of formal power series is given. All Lie bialgebra structures on the Lie algebra {\Cal G}_{\infty} of $G_{\infty}$ are also classified.Comment: 11 pages, AmSTeX fil

The fecal resistome of dairy cattle is associated with diet during nursing.

Antimicrobial resistance is a global public health concern, and livestock play a significant role in selecting for resistance and maintaining such reservoirs. Here we study the succession of dairy cattle resistome during early life using metagenomic sequencing, as well as the relationship between resistome, gut microbiota, and diet. In our dataset, the gut of dairy calves serves as a reservoir of 329 antimicrobial resistance genes (ARGs) presumably conferring resistance to 17 classes of antibiotics, and the abundance of ARGs declines gradually during nursing. ARGs appear to co-occur with antibacterial biocide or metal resistance genes. Colostrum is a potential source of ARGs observed in calves at day 2. The dynamic changes in the resistome are likely a result of gut microbiota assembly, which is closely associated with diet transition in dairy calves. Modifications in the resistome may be possible via early-life dietary interventions to reduce overall antimicrobial resistance

Magnetization Process of Nanoscale Iron Cluster

Low-temperature magnetization process of the nanoscale iron cluster in linearly sweeped fields is investigated by a numerical analysis of time-dependent Schr$\ddot{\rm o}$dinger equation and the quantum master equation. We introduce an effective basis method extracting important states, by which we can obtain the magnetization process effectively. We investigate the structure of the field derivative of the magnetization. We find out that the antisymmetric interaction determined from the lattice structure reproduces well the experimental results of the iron magnets and that this interaction plays an important role in the iron cluster. Deviations from the adiabatic process are also studied. In the fast sweeping case, our calculations indicate that the nonadiabatic transition dominantly occurs at the level crossing for the lowest field. In slow sweeping case, due to the influence of the thermal environment to the spin system, the field derivative of the magnetization shows an asymmetric behavior, the magnetic F$\ddot{\rm o}$hn effect, which explains the substructure of the experimental results in the pulsed field.Comment: 5 pages of text and 2 pages of 6 figures. To appear in J. Phys. Soc. Jp

Controlling Silver Nanoparticle Size and Morphology with Photostimulated Synthesis

Photo-induced synthesis and control over the size and shape of colloidal silver nanoparticles is investigated in contrast to photo-stimulated aggregation of small nanoparticles into large fractal-type structures. The feasibility of light-driven nanoengineering which enables manipulation of the sizes and shapes of the isolated nanoparticles is studied by varying the amount and type of the stabilizing agent and the type of optical irradiation.Comment: 10 pages, 7 figures, 11 image

The relationship between transcription initiation RNAs and CCCTC-binding factor (CTCF) localization

Background: Transcription initiation RNAs (tiRNAs) are nuclear localized 18 nucleotide RNAs derived from sequences immediately downstream of RNA polymerase II (RNAPII) transcription start sites. Previous reports have shown that tiRNAs are intimately correlated with gene expression, RNA polymerase II binding and behaviors, and epigenetic marks associated with transcription initiation, but not elongation. Results: In the present work, we show that tiRNAs are commonly found at genomic CCCTC-binding factor (CTCF) binding sites in human and mouse, and that CTCF sites that colocalize with RNAPII are highly enriched for tiRNAs. To directly investigate the relationship between tiRNAs and CTCF we examined tiRNAs originating near the intronic CTCF binding site in the human tumor suppressor gene, p21 (cyclin-dependent kinase inhibitor 1A gene, also known as CDKN1A). Inhibition of CTCF-proximal tiRNAs resulted in increased CTCF localization and increased p21 expression, while overexpression of CTCF-proximal tiRNA mimics decreased CTCF localization and p21 expression. We also found that tiRNA-regulated CTCF binding influences the levels of trimethylated H3K27 at the alternate upstream p21 promoter, and affects the levels of alternate p21 (p21) transcripts. Extending these studies to another randomly selected locus with conserved CTCF binding we found that depletion of tiRNA alters nucleosome density proximal to sites of tiRNA biogenesis. Conclusions: Taken together, these data suggest that tiRNAs modulate local epigenetic structure, which in turn regulates CTCF localization

Screening women for intimate partner violence in healthcare settings: abridged Cochrane systematic review and meta-analysis

Objective To examine the effectiveness of screening for intimate partner violence conducted within healthcare settings to determine whether or not screening increases identification and referral to support agencies, improves women’s wellbeing, decreases further violence, or causes harm. Design Systematic review and meta-analysis of trials assessing effectiveness of screening. Study assessment, data abstraction, and quality assessment were conducted independently by two of the authors. Standardised estimations of the risk ratios and 95% confidence intervals were calculated. Data sources Nine databases searched up to July 2012 (CENTRAL, Medline, Medline(R), Embase, DARE, CINAHL, PsycINFO, Sociological Abstracts, and ASSIA), and five trials registers searched up to 2010. Eligibility criteria for selecting studies Randomised or quasi-randomised trials of screening programmes for intimate partner violence involving all women aged ≥16 attending a healthcare setting. We included only studies in which clinicians in the intervention arm personally conducted the screening, or were informed of the screening result at the time of the consultation, compared with usual care (or no screening). Studies of screening programmes that were followed by structured interventions such as advocacy or therapeutic intervention were excluded. Results 11 eligible trials (n=13 027) were identified. In six pooled studies (n=3564), screening increased the identification of intimate partner violence (risk ratio 2.33, 95% confidence interval 1.39 to 3.89), particularly in antenatal settings (4.26, 1.76 to 10.31). Based on three studies (n=1400), we detected no evidence that screening increases referrals to domestic violence support services (2.67, 0.99 to 7.20). Only two studies measured women’s experience of violence after screening (three to 18 months after screening) and found no reduction in intimate partner violence. One study reported that screening does not cause harm. Conclusions Though screening is likely to increase identification of intimate partner violence in healthcare settings, rates of identification from screening interventions were low relative to best estimates of prevalence of such violence. It is uncertain whether screening increases effective referral to supportive agencies. Screening does not seem to cause harm in the short term, but harm was measured in only one study. As the primary studies did not detect improved outcomes for women screened for intimate partner violence, there is insufficient evidence for screening in healthcare settings. Studies comparing screening versus case finding, or screening in combination with therapeutic intervention for women’s long term wellbeing, are needed to inform the implementation of identification policies in healthcare settings

A Survey for Low-Surface-Brightness Galaxies Around M31. I. The Newly Discovered Dwarf Andromeda V

We present images and a color-magnitude diagram for And V, a new dwarf spheroidal companion to M31 that was found using a digital filtering technique applied to 1550 square degrees of the second Palomar Sky Survey. And V resolves into stars easily in follow-up 4-m V- and I-band images, from which we deduce a distance of 810 +/- 45 kpc using the tip of the red giant branch method. Within the uncertainties, this distance is identical to the Population II distances for M31 and, combined with a projected separation of 112 kpc, provides strong support for a physical association between the two galaxies. There is no emission from And V detected in H alpha, 1.4 GHz radio continuum, or IRAS bandpasses, and there is no young population seen in the color-magnitude diagram that might suggest that And V is an irregular. Thus, the classification as a new dwarf spheroidal member of the Local Group seems secure. With an extinction-corrected central surface brightness of 25.2 V mag per square arcsec, a mean metal abundance of [Fe/H] approximately -1.5, and no evidence for upper AGB stars, And V resembles And I & III.Comment: Accepted for publication in The Astronomical Journal, November 1998 issue; 4 embedded PostScript figures, 4 JPEG figures; see http://aloe.tuc.noao.edu/jacoby/dwarfs.html for a complete full-resolution PostScript versio