20 research outputs found
Metafluid dynamics and Hamilton-Jacobi formalism
Metafluid dynamics was investigated within Hamilton-Jacobi formalism and the
existence of the hidden gauge symmetry was analyzed. The obtained results are
in agreement with those of Faddeev-Jackiw approach.Comment: 7 page
LYSOSOME-DEPENDENT CELL DEATH DEFINES SPECIFIC ENDOTHELIAL TOXICITY OF CALCIUM PHOSPHATE BIONS
Aim of the study was to identify the mechanism of specific endothelial toxicity related to calcium phosphate bions (CPB). Material and methods. CPB and magnesium phosphate bions (MPB) were artificially synthesised through supersaturation of culture medium with respective salts and then added to human endothelial cells (EA.hy 926) and murine endothelial cells (2H-11) to study: 1) spatiotemporal aspects of bion internalisation by means of transmission electron microscopy and confocal microscopy; 2) whether blocking of H+-ATPase by lysosomal inhibitor bafilomycin A1 affects endothelial toxicity of bions; 3) expression of caspase-3 and its substrate poly(ADP-ribose) polymerase (PARP-1). Results. CPB were internalized by endothelial cells as early as 1 h upon their addition and were localized in lysosomes; after 4 h, we detected release of calcium ions (Ca2+) from lysosomes to cytosol accompanied by multifold increase in cleaved caspase 3 and its substrate PARP-1. Bafilomycin A1 rescued endothelial cells from death induced by slightly soluble CPB regardless of exposure time and dose; however, freely soluble MPB did not evince endothelial toxicity regardless of bafilomycin A1 addition. Conclusion. Upon internalization by endothelial cells, CPB cause their death due to dissolution in lysosomes and subsequent release of calcium ions into the cytosol, ultimately leading to cleavage of executioner caspases. MPB lack endothelial toxicity because their dissolution does not lead to release of calcium ions. Therefore, specific endothelial toxicity of CPB is defined by lysosome-dependent cell death
Structure of Core-Shell Ni/Au Nanoparticles Synthesized in Two-Stage Process From Aqueous Salt Solutions
Core-shell Ni/Au nanoparticles are synthesized in a two-stage process in aqueous solutions. The core-shell structure of the synthesized particles is proven by using results of HRTEM, TEM, SAXS and CVA methods. The thickness of the core is estimated at 1 nm and it is formed from 1–2 layers of mutually oriented gold crystallites. The CVA results show that the Au-shells are nanoporous, and that a layer of nickel oxidation products is formed. These results were obtained by using Vis spectrophotometer, TEM, HRTEM, SAXS, CVA and elemental analysis
Analysis of the efficiency of CVD synthesis of carbon nanotubes by the aerosol method based on ethanol
Analysis of the efficiency of CVD synthesis of carbon nanotubes by the aerosol method based on ethanol
Conductivity of thin films based on single-walled carbon nanotubes grown by chemical vapor deposition
Electrical and optical properties of thin films of single-walled carbon nanotubes (SWCNT) obtained by aerosol chemical vapor deposition using ethanol, ferrocene, and sulfur are studied. Structural and geometrical characteristics of the synthesis products are determined by the methods of Raman spectroscopy and transmission electron microscopy. The effect of sulfur on the properties of the SWCNTs and thin films based on them is found
Conductivity of thin films based on single-walled carbon nanotubes grown by chemical vapor deposition
Electrical and optical properties of thin films of single-walled carbon nanotubes (SWCNT) obtained by aerosol chemical vapor deposition using ethanol, ferrocene, and sulfur are studied. Structural and geometrical characteristics of the synthesis products are determined by the methods of Raman spectroscopy and transmission electron microscopy. The effect of sulfur on the properties of the SWCNTs and thin films based on them is found
