275 research outputs found
Dynamics of proteins: Light scattering study of dilute and dense colloidal suspensions of eye lens homogenates
We report a dynamic light scattering study on protein suspensions of bovine
lens homogenates at conditions (pH and ionic strength) similar to the
physiological ones. Light scattering data were collected at two temperatures,
20 oC and 37 oC, over a wide range of concentrations from the very dilute limit
up to the dense regime approaching to the physiological lens concentration. A
comparison with experimental data from intact bovine lenses was advanced
revealing differences between dispersions and lenses at similar concentrations.
In the dilute regime two scattering entities were detected and identified with
the long-time, self-diffusion modes of alpha-crystallins and their aggregates,
which naturally exist in lens nucleus. Self-diffusion coefficients are
temperature insensitive, whereas the collective diffusion coefficient depends
strongly on temperature revealing a reduction of the net repulsive
interparticle forces with lowering temperature. While there are no rigorous
theoretical approaches on particle diffusion properties for multi-component,
non-ideal hard-sphere, polydispersed systems, as the suspensions studied here,
a discussion of the volume fraction dependence of the long-time, self-diffusion
coefficient in the context of existing theoretical approaches was undertaken.
This study is purported to provide some insight into the complex light
scattering pattern of intact lenses and the interactions between the
constituent proteins that are responsible for lens transparency. This would
lead to understand basic mechanisms of specific protein interactions that lead
to lens opacification (cataract) under pathological conditions.Comment: To appear in J. Chem. Phy
Instantaneous responses of microbial communities to stress in soils pretreated with Mentha spicata essential oil and/or inoculated with arbuscular mycorrhizal fungus
Monitoring of a methane-seeping pockmark by cabled benthic observatory (Patras Gulf, Greece)
A new seafloor observatory, the gas monitoring
module (GMM), has been developed for continuous and
long-term measurements of methane and hydrogen sulphide
concentrations in seawater, integrated with temperature (T),
pressure (P) and conductivity data at the seafloor. GMM
was deployed in April 2004 within an active gas-bearing
pockmark in the Gulf of Patras (Greece), at a water depth of
42 m. Through a submarine cable linked to an onshore
station, it was possible to remotely check, via direct phone
connection, GMM functioning and to receive data in nearreal
time. Recordings were carried out in two consecutive
campaigns over the periods AprilâJuly 2004, and September
2004âJanuary 2005, amounting to a combined dataset
of ca. 6.5 months. This represents the first long-term
monitoring ever done on gas leakage from pockmarks by
means of CH4+H2S+T+P sensors. The results show
frequent T and P drops associated with gas peaks, more
than 60 events in 6.5 months, likely due to intermittent,
pulsation-like seepage. Decreases in temperature in the
order of 0.1â1°C (up to 1.7°C) below an ambient T of ca.
17°C (annual average) were associated with short-lived
pulses (10â60 min) of increased CH4+H2S concentrations.
This seepage âpulsationâ can either be an active process
driven by pressure build-up in the pockmark sediments, or a
passive fluid release due to hydrostatic pressure drops
induced by bottom currents cascading into the pockmark
depression. Redundancy and comparison of data from
different sensors were fundamental to interpret subtle proxy
signals of temperature and pressure which would not be
understood using only one sensor.Published297-302JCR Journalreserve
Enthesiale Veraenderungen (Entheseal changes), In: Neue Erkenntnisse zur frĂźhmittelalterlichen Separatgrablege von Niederstotzingen, Kreis Heidenheim (New results from the cemetery of Niederstotzingen)
Τhe soundscape of the Inner Ionian Archipelago as evinced through the West Patraikos Gulf Ambient and Seismic Noise Monitoring Project
Case Report Bilateral Breast Metastases from Vulvar Carcinoma: A Case Report and Literature Review
Vulvar carcinoma is a rare disease that accounts for 3-5% of all gynecologic malignancies. Breast represents an unusual site of metastasis and only a few cases are reported. We describe the first case of bilateral metastatic breast carcinoma of vulvar origin, at an 80-year-old female patient. Six months after treatment of her primary disease, she presented with bilateral metastatic squamous cell breast carcinoma. Diagnosis was based on clinical, radiological, and histological facts. Breast although rare is another potential site of metastasis in vulvar cancer and thus mammary gland examination should be considered in the follow-up of these patients. Differential diagnosis between primary and metastatic lesions is of utmost importance for appropriate management
Hadron therapy information sharing prototype
The European PARTNER project developed a prototypical system for sharing hadron therapy data. This system allows doctors and patients to record and report treatment-related events during and after hadron therapy. It presents doctors and statisticians with an integrated view of adverse events across institutions, using open-source components for data federation, semantics, and analysis. There is a particular emphasis upon semantic consistency, achieved through intelligent, annotated form designs. The system as presented is ready for use in a clinical setting, and amenable to further customization. The essential contribution of the work reported here lies in the novel data integration and reporting methods, as well as the approach to software sustainability achieved through the use of community-supported open-source components
Raman scattering study of the a-GeTe structure and possible mechanism for the amorphous-to-crystal transition
We report on an inelastic (Raman) light scattering study of the local
structure of amorphous GeTe films. A detailed analysis of the
temperature-reduced Raman spectra has shown that appreciable structural changes
occur as a function of temperature. These changes involve modifications of
atomic arrangements such as to facilitate the rapid amorphous-to-crystal
transformation, which is the major advantage of phase-change materials used in
optical data storage media. A particular structural model, supported by
polarization analysis, is proposed being compatible with the experimental data
as regards both the structure of a-GeTe and the crystallization transition. The
remarkable difference between the Raman spectrum of the crystal and the glass
can thus naturally be accounted for.Comment: Published in: J. Phys. Condens. Matter. 18, 965-979 (2006
Dynamic light scattering study on phase separation of a protein-water mixture: Application on cold cataract development in the ocular lens
We present a detailed dynamic light scattering study on the phase separation
in the ocular lens emerging during cold cataract development. Cold cataract is
a phase separation effect that proceeds via spinodal decomposition of the lens
cytoplasm with cooling. Intensity auto-correlation functions of the lens
protein content are analyzed with the aid of two methods providing information
on the populations and dynamics of the scattering elements associated with cold
cataract. It is found that the temperature dependence of many measurable
parameters changes appreciably at the characteristic temperature ~16+1 oC which
is associated with the onset of cold cataract. Extending the temperature range
of this work to previously inaccessible regimes, i.e. well below the phase
separation or coexistence curve at Tcc, we have been able to accurately
determine the temperature dependence of the collective and self-diffusion
coefficient of proteins near the spinodal. The analysis showed that the
dynamics of proteins bears some resemblance to the dynamics of structural
glasses where the apparent activation energy for particle diffusion increases
below Tcc indicating a highly cooperative motion. Application of ideas
developed for studying the critical dynamics of binary protein/solvent
mixtures, as well as the use of a modified Arrhenius equation, enabled us to
estimate the spinodal temperature Tsp of the lens nucleus. The applicability of
dynamic light scattering as a non-invasive, early-diagnostic tool for ocular
diseases is also demonstrated in the light of the findings of the present
paper
Measuring co-authorship and networking-adjusted scientific impact
Appraisal of the scientific impact of researchers, teams and institutions
with productivity and citation metrics has major repercussions. Funding and
promotion of individuals and survival of teams and institutions depend on
publications and citations. In this competitive environment, the number of
authors per paper is increasing and apparently some co-authors don't satisfy
authorship criteria. Listing of individual contributions is still sporadic and
also open to manipulation. Metrics are needed to measure the networking
intensity for a single scientist or group of scientists accounting for patterns
of co-authorship. Here, I define I1 for a single scientist as the number of
authors who appear in at least I1 papers of the specific scientist. For a group
of scientists or institution, In is defined as the number of authors who appear
in at least In papers that bear the affiliation of the group or institution. I1
depends on the number of papers authored Np. The power exponent R of the
relationship between I1 and Np categorizes scientists as solitary (R>2.5),
nuclear (R=2.25-2.5), networked (R=2-2.25), extensively networked (R=1.75-2) or
collaborators (R<1.75). R may be used to adjust for co-authorship networking
the citation impact of a scientist. In similarly provides a simple measure of
the effective networking size to adjust the citation impact of groups or
institutions. Empirical data are provided for single scientists and
institutions for the proposed metrics. Cautious adoption of adjustments for
co-authorship and networking in scientific appraisals may offer incentives for
more accountable co-authorship behaviour in published articles.Comment: 25 pages, 5 figure
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