52 research outputs found
MICROPROCESSOR-CONTROLLED GAS ANALYSER SYSTEM FOR MULTI-CHANNEL MASS SPECTROMETRIC MEASUREMENT OF PLANT GASES
A CRISPR/Cas9-based multicopy integration system for protein production in Aspergillus niger
The filamentous fungus Aspergillus niger is well known for its high protein secretion capacity and a preferred host for homologous and heterologous protein production. To improve the protein production capacity of A. niger even further, a set of dedicated protein production strains was made containing up to ten glucoamylase landing sites (GLSs) at predetermined sites in the genome. These GLSs replace genes encoding enzymes abundantly present or encoding unwanted functions. Each GLS contains the promotor and terminator region of the glucoamylase gene (glaA), one of the highest expressed genes in A. niger. Integrating multiple gene copies, often realized by random integration, is known to boost protein production yields. In our approach the GLSs allow for rapid targeted gene replacement using CRISPR/Cas9-mediated genome editing. By introducing the same or different unique DNA sequences (dubbed KORE sequences) in each GLS and designing Cas9-compatible single guide RNAs, one is able to select at which GLS integration of a target gene occurs. In this way a set of identical strains with different copy numbers of the gene of interest can be easily and rapidly made to compare protein production levels. As an illustration of its potential, we successfully used the expression platform to generate multicopy A. niger strains producing the Penicillium expansum PatE::6xHis protein catalyzing the final step in patulin biosynthesis. The A. niger strain expressing ten copies of the patE::6xHis expression cassette produced about 70 μg/mL PatE protein in the culture medium with a purity just under 90%.</p
Antiulcer activity of fluvoxamine in rats and its effect on oxidant and antioxidant parameters in stomach tissue
Potential Phytopharmacy and Food Applications of Capsicum spp.: A Comprehensive Review
Capsicum genus (Solanaceae) is native to the Americas. Today, it is an important agricultural crop cultivated around the world, not only due to its economic importance, but also for the nutritional value of the fruits. Among their phytochemical constituents, capsaicinoids are characteristic and responsible of the pungency of sharp-tasting cultivars. Moreover, Capsicum and capsaicinoids (mainly, capsaicin) have been largely studied because of their health benefits. Thus, this study reviews the scientific knowledge about Capsicum spp. and their phytochemicals against cancer, diabetes, gastrointestinal diseases, pain, and metabolic syndrome, as well as their antioxidant and antimicrobial activity. These bioactivities can be the basis of the formulation of functional ingredients and natural preservatives containing Capsicum extracts or isolated compounds
Cellular mechanisms of β-carotene-induced gastric cytoprotection in indomethacin-treated rats
Joint business meeting of the International Conference on Ulcer Research (ICUR) and gastrointestinal section of the International Union of Pharmacology (GI section of IUPHAR) in Osaka, Japan, July 9, 2006
Gastric mucosal preventive effects of prostacyclin and β-carotene, and their biochemical effects in rats treated with ethanol and HCl at different doses and time intervals after administration of necrotizing agents
Examination of stomach wall Mg2+?Na+?K+-dependent ATPase, ATP, and ADP in pylorus-ligated rats
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