Effect of fumaric acid, calcium formate and mineral levels in diets on the intake and growth performance of newly weaned pigs

peer-reviewedThe weaned pig has limited ability to acidify its stomach contents. The objective of this study (comprising three experiments) was to examine the effect of feeding diets containing fumaric acid (FA), calcium formate (CF) or diets of low acid binding capacity (ABC) on post-weaning pig performance. In all three experiments, pigs (10 per treatment) were weaned at 19 to 24 days, blocked on sex and weight and assigned at random to one of six treatments. In Experiment 1, treatments were: (1) control diet, (2) control 20 g/kg FA, (3) control 15 g/kg CF, (4) low Ca (2.8 g/kg) and P (5.1 g/kg) (LCaP) diet for seven days followed by the control diet, (5) LCaP diet for seven days followed by control 20 g/kg FA, and (6) LCaP diet for seven days followed by control 15 g/kg CF. In Experiment 2, treatments were: (1) control diet, (2) control 20 g/kg FA, (3) control 15 g/kg CF, (4) LCaP diet for 14 days followed by the control diet, (5) LCaP diet for 14 days followed by control 20 g/kg FA, and (6) LCaP diet for seven days followed by control diet. In Experiment 3, treatments were: (1) high Ca (HC) diet (12 g/kg), (2) medium Ca (MC) diet (9 g/kg), (3) low Ca (LC) diet (6 g/kg), (4) HC 20 g/kg FA, (5) MC 20 g/kg FA, and (6) LC 20 g/kg FA. Pigs were individually fed for 26 days. In Experiment 1, CF tended to depress daily feed intake (DFI) in the final two weeks (691 v. 759 and 749, (s.e. 19) g/day, P = 0.07) and overall average daily gain (322 v. 343 and 361 (s.e. 11) g/day, P = 0.09) compared with the control and FA supplemented diets, respectively. Feeding diets with LCaP for seven days post weaning increased DFI (208 v. 178, (s.e. 8) g/day, P < 0.01) in week 1 and tended to improve feed conversion rate in the first two weeks (1.65 v. 1.85, s.e. 0.10, P = 0.09). In Experiment 2, treatment had no significant effect on pig performance but feed conversion rate in weeks three and four was improved for Treatment 5 compared with Treatment 4 (1.30 v. 1.39 (s.e. 0.06) g/g, P < 0.01). In experiment 3, FA increased (P < 0.05) pig weight at day 14 (8.4 v. 7.7 (s.e. 0.2) kg) and feed intake in weeks one and two (223 v. 251, (s.e. 9) g/day). It is concluded that CF did not improve performance but reducing diet ABC or including FA in the diet did improve performance

Obstetrician-assessed maternal health at pregnancy predicts offspring future health

Background: We aimed to examine the association between obstetrician assessment of maternal physical health at the time of pregnancy and offspring cardiovascular disease risk.&lt;p&gt;&lt;/p&gt; Methods and Principal Findings: We examined this association in a birth cohort of 11,106 individuals, with 245,000 person years of follow-up. We were concerned that any associations might be explained by residual confounding, particularly by family socioeconomic position. In order to explore this we used multivariable regression models in which we adjusted for a range of indicators of socioeconomic position and we explored the specificity of the association. Specificity of association was explored by examining associations with other health related outcomes. Maternal physical health was associated with cardiovascular disease: adjusted (socioeconomic position, complications of pregnancy, birthweight and childhood growth at mean age 5) hazard ratio comparing those described as having poor or very poor health at the time of pregnancy to those with good or very good health was 1.55 (95%CI: 1.05, 2.28) for coronary heart disease, 1.91 (95%CI: 0.99, 3.67) for stroke and 1.57 (95%CI: 1.13, 2.18) for either coronary heart disease or stroke. However, this association was not specific. There were strong associations for other outcomes that are known to be related to socioeconomic position (3.61 (95%CI: 1.04, 12.55) for lung cancer and 1.28 (95%CI:1.03, 1.58) for unintentional injury), but not for breast cancer (1.10 (95%CI:0.48, 2.53)).&lt;p&gt;&lt;/p&gt; Conclusions and Significance: These findings demonstrate that a simple assessment of physical health (based on the appearance of eyes, skin, hair and teeth) of mothers at the time of pregnancy is a strong indicator of the future health risk of their offspring for common conditions that are associated with poor socioeconomic position and unhealthy behaviours. They do not support a specific biological link between maternal health across her life course and future risk of cardiovascular disease in her offspring.&lt;p&gt;&lt;/p&gt

Enhancement of pigmeat quality by altering pre-slaughter management

End of project reportThe studies presented in this report were conducted to investigate the effect of breed, slaughter weight, castration of male pigs and strategic feeding strategies on the performance of pigs to slaughter and on their carcass quality. The effect of breed, gender and feeding regimen on the performance of pigs and their carcass quality was examined in the first study (Section 3). From weaning to slaughter Landrace-sired pigs grew at a similar rate but had a better feed conversion efficiency compared with Duroc-sired pigs. Landrace-sired pigs also had a higher carcass lean and greater muscle depth than Duroc-sired pigs. Entire male pigs grew more efficiently, had lower lean content in their carcasses and had a reduced kill out yield when compared with gilts. The eye muscle depth was greater for gilts than entire males. Diluting the diet with grass-meal (GM) reduced growth rate, caused a deterioration in feed conversion efficiency, reduced back fat thickness, reduced eye muscle thickness and reduced kill out yield compared to the control feeding regimen of a cereal based diet. Compensatory growth was observed during a re-alimentation period following a period of diet dilution with grass-meal. However, where it did occur, in most cases it was only partial. Adding 5% rapeseed oil instead of lard to the finisher diet increased nitrogen utilization efficiency and phosphorous utilization efficiency. The effect of gender (boar, castrate, gilt) and slaughter weight (80 to 120kg) on pig performance, carcass quality, meat quality, and nitrogen excretion was investigated in the second study (Section 4). Boars grew faster than gilts and more efficiently than castrates or gilts. Castrates had a higher kill out yield than boars. Nitrogen excretion from castrates was similar to gilts which were both higher than that from boars. The processing value of carcasses from castrates may be higher than that of boars and gilts. In particular castrates had heavier loins and bellies than either boars or gilts. Carcasses from castrates and gilts had a higher temperature (recorded 24 hours post slaughter) than boars. However, pH24 was not affected by gender. The intramuscular fat content of the l. dorsi in castrates was higher than that of boars or gilts, however at 1.65% this was well below the level (2.0%) above which any noticeable sensory attributes might be detected. Feed intake increased with increasing slaughter weight and feed conversion efficiency deteriorated. N excretion also increased with each increment in weight. Carcass lean content increased up to 90kg live EOP 4939.doc 4 25/10/2005 weight then reached a plateau and declined after 110kg live weight. Heavier carcasses yielded more product for approximately the same slaughtering cost and the associated larger muscles could make it easier to use seam butchery techniques that result in lean, well-trimmed, attractive cuts and joints. The pH45 and pH24 were reduced with increasing slaughter weight and drip loss increased. Heavier pigs may be more prone to the development of PSE than lighter pigs as their carcass temperature remains higher for longer than that of lighter pigs

Studies on growth rates in pigs and the effect of birth weight

End of project reportThe purpose of this study was to assess some environmental and management factors that affect growth performance on commercial pig units. In experiment 1, a survey was carried out on 22 pig units of known growth performance in south-west Ireland to compare management factors between those showing poor and good growth rates. Low growth rate appears to be due to the cumulative effect of a combination of factors. Experiment 2 was conducted to determine the effects of providing an additional feeder on performance of weaned piglets. No benefits were recorded. Feed consumed from the additional feeder was a replacement for feed that otherwise would have been consumed from the control hopper feeder. Experiment 3 was designed to determine if pig performance and efficiency of growth were affected by weight at birth and at weaning. Lightweight pigs showed inferior growth performance up to the finisher period. Although they compensated some of the inferior growth towards the time of slaughter, they never reached the weights of the heavy birth-weight animals. Males were either significantly heavier or tended to be heavier than females throughout. There was no significant difference between the sexes in the number of days to slaughter. Light and heavy pigs did not differ in the levels of IGF-1 in their blood plasma; however lightweight pigs had significantly lower IgG preweaning. Experiment 4 aimed to determine whether piglet birth weight influenced growth performance, plasma IGF-1 concentrations and muscle fibre characteristics at day 42 of life. At slaughter (Day 42) light birth weight pigs were significantly (P < 0.001) lighter. Plasma IGF-1 concentration was lower by 28% (P=0.06) in light pigs. Muscle fibre cross sectional area and total fibre number were not significantly different between groups. This study should be repeated with bigger numbers

Use of methotrexate in inflammatory bowel disease in 2014: A User\u27s Guide

Methotrexate has been used an immunomodulator in many autoimmune diseases, including inflammatory bowel disease. However, many physicians are unfamiliar or uncomfortable with its use in the management of inflammatory bowel disease. We summarize the data for use of methotrexate in common clinical scenarios: (1) steroid dependant Crohn\u27s disease (CD); (2) maintenance of remission in steroid free CD; (3) azathioprine failures in CD; (4) in combination therapy with Anti-TNF agents in CD; (5) decreasing antibody formation to Anti-TNF therapy in CD; (6) management of fistulizing disease in CD; and (7) as well as induction and maintenance of remission in ulcerative colitis. An easy to use algorithm is provided for the busy clinician to access and safely prescribe methotrexate for their inflammatory bowel disease patients

Salmonella in Irish pig farms; prevalence, antibiotic resistance and molecular epidemiology

The objective was to examine the prevalence of Salmonella in manure from 30 Irish pig farms and to characterize any recovered isolates in order to assess potential risks and epidemiological relationships. Salmonella was detected in the manure from finisher pigs in 50% of the herds investigated. S. Typhimurium was the predominant serotype recovered and the most common phage types were DT104 and DT104b. Nineteen of the 29 Salmonella isolates recovered were resistant to one or more antibiotics and 15 of these (all Typhimurium) were multi-resistant

Offspring subcutaneous adipose markers are sensitive to the timing of maternal gestational weight gain

peer-reviewedBackground Excessive maternal weight gain during pregnancy impacts on offspring health. This study focused on the timing of maternal gestational weight gain, using a porcine model with mothers of normal pre-pregnancy weight. Methods Trial design ensured the trajectory of maternal gestational weight gain differed across treatments in early, mid and late gestation. Diet composition did not differ. On day 25 gestation, sows were assigned to one of five treatments: Control sows received a standard gestation diet of 2.3 kg/day (30 MJ DE/day) from early to late gestation (day 25–110 gestation). E sows received 4.6 kg food/day in early gestation (day 25–50 gestation). M sows doubled their food intake in mid gestation (day 50–80 gestation). EM sows doubled their food intake during both early and mid gestation (day 25–80 gestation). L sows consumed 3.5 kg food/day in late gestation (day 80–110 gestation). Offspring body weight and food intake levels were measured from birth to adolescence. Markers of lipid metabolism, hypertrophy and inflammation were investigated in subcutaneous adipose tissue of adolescent offspring. Results The trajectory of gestational weight gain differed across treatments. However total gestational weight gain did not differ except for EM sows who were the heaviest and fattest mothers at parturition. Offspring birth weight did not differ across treatments. Subcutaneous adipose tissue from EM offspring differed significantly from controls, with elevated mRNA levels of lipogenic (CD36, ACACB and LPL), nutrient transporters (FABP4 and GLUT4), lipolysis (HSL and ATGL), adipocyte size (MEST) and inflammation (PAI-1) indicators. The subcutaneous adipose depot from L offspring exhibited elevated levels of CD36, ACACB, LPL, GLUT4 and FABP4 mRNA transcripts compared to control offspring. Conclusions Increasing gestational weight gain in early gestation had the greatest impact on offspring postnatal growth rate. Increasing maternal food allowance in late gestation appeared to shift the offspring adipocyte focus towards accumulation of fat. Mothers who gained the most weight during gestation (EM mothers) gave birth to offspring whose subcutaneous adipose tissue, at adolescence, appeared hyperactive compared to controls. This study concluded that mothers, who gained more than the recommended weight gain in mid and late gestation, put their offspring adipose tissue at risk of dysfunction.This research was funded by Teagasc, under the National Development Plan. LBMcN was in receipt of a Teagasc Walsh Fellowship. Nestle hosted LG on a sabbatical and funded the RT-PCR cost

Association of plasma uric acid with ischaemic heart disease and blood pressure: mendelian randomisation analysis of two large cohorts

Objectives: To assess the associations between both uric acid levels and hyperuricaemia, with ischaemic heart disease and blood pressure, and to explore the potentially confounding role of body mass index. Design: Mendelian randomisation analysis, using variation at specific genes (SLC2A9 (rs7442295) as an instrument for uric acid; and FTO (rs9939609), MC4R (rs17782313), and TMEM18 (rs6548238) for body mass index). Setting: Two large, prospective cohort studies in Denmark. Participants: We measured levels of uric acid and related covariables in 58 072 participants from the Copenhagen General Population Study and 10 602 from the Copenhagen City Heart Study, comprising 4890 and 2282 cases of ischaemic heart disease, respectively. Main outcome: Blood pressure and prospectively assessed ischaemic heart disease. Results: Estimates confirmed known observational associations between plasma uric acid and hyperuricaemia with risk of ischaemic heart disease and diastolic and systolic blood pressure. However, when using genotypic instruments for uric acid and hyperuricaemia, we saw no evidence for causal associations between uric acid, ischaemic heart disease, and blood pressure. We used genetic instruments to investigate body mass index as a potentially confounding factor in observational associations, and saw a causal effect on uric acid levels. Every four unit increase of body mass index saw a rise in uric acid of 0.03 mmol/L (95% confidence interval 0.02 to 0.04), and an increase in risk of hyperuricaemia of 7.5% (3.9% to 11.1%). Conclusion: By contrast with observational findings, there is no strong evidence for causal associations between uric acid and ischaemic heart disease or blood pressure. However, evidence supports a causal effect between body mass index and uric acid level and hyperuricaemia. This finding strongly suggests body mass index as a confounder in observational associations, and suggests a role for elevated body mass index or obesity in the development of uric acid related conditions