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    Π˜ΡΡ‚ΠΎΡ€ΠΈΠΊ, архивист, Π±ΠΈΠ±Π»ΠΈΠΎΠ³Ρ€Π°Ρ„: П.А. Зайончковский ΠΈ Российская государствСнная Π±ΠΈΠ±Π»ΠΈΠΎΡ‚Π΅ΠΊΠ°

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    At present, when the majority of users in search for information excessively turn to the Internet as a primary data source, the problem of reliability of the obtained data is particularly acute. Libraries, whose main function is to store and provide access to information existing both in print and electronic forms, traditionally tend to maintain the exceptional level of completeness and validity of the data and sources provided. One of the ways to achieve such reliability is interaction of libraries with scientists in the field of data systematization, verification, storage and access providing. The article analyses the scientific work of the outstanding Russian historian P.A. Zaionchkovskij in the V.I. Lenin State Library of the USSR (now β€” the Russian State Library, RSL) and considers his scientific cooperaction with the Library for several decades as a successful example of library-scientists’ partnership. In 1944β€”1952, he served as the Head of the Department of manuscripts of the V.I. Lenin State Library. Under his supervision, the accounting of the Department’s stock was reorganized, preservation of the existing and newly acquired collections was ensured; the user access of researches to the information on the collections’ content and composition was significantly improved; the unique team of scientific personnel was formed. The article gives thorough overview of the scientific and publication activity of the Department during that period. The authors also review the contribution of P.A. Zaionchkovskij to the development of the problem of bibliographic providing for historical science. In the 1960s β€” 1980s, under his scientific supervision, the bibliographic staff of the V.I. Lenin State Library of the USSR and other major Russian libraries prepared the unique publications β€” β€œReference Books on the History of Pre-revolutionary Russia” and multivolume annotated Index β€œThe History of Pre-revolutionary Russia in Diaries and Memoirs”. P.A. Zaionchkovskij laid the foundation for the lasting many years and still developing project on creation of retrospective scientific auxiliary indices of memoir literature. The partnership of P.A. Zaionchkovskij and the V.I. Lenin State Library is an excellent example of the successful and fruitful interaction of scientists and library staff.Π’ настоящСС врСмя, ΠΊΠΎΠ³Π΄Π° ΠΏΠΎΠ»ΡŒΠ·ΠΎΠ²Π°Ρ‚Π΅Π»ΠΈ Π² поисках ΠΈΠ½Ρ„ΠΎΡ€ΠΌΠ°Ρ†ΠΈΠΈ ΠΎΠ±Ρ€Π°Ρ‰Π°ΡŽΡ‚ΡΡ Ρ‡Π°Ρ‰Π΅ всСго ΠΊ Π˜Π½Ρ‚Π΅Ρ€Π½Π΅Ρ‚Ρƒ, ΠΏΡ€ΠΎΠ±Π»Π΅ΠΌΠ° достовСрности ΠΏΠΎΠ»ΡƒΡ‡Π΅Π½Π½Ρ‹Ρ… Π΄Π°Π½Π½Ρ‹Ρ… стоит особСнно остро. Π‘ΠΈΠ±Π»ΠΈΠΎΡ‚Π΅ΠΊΠΈ, вСдущая функция ΠΊΠΎΡ‚ΠΎΡ€Ρ‹Ρ… β€” Ρ…Ρ€Π°Π½Π΅Π½ΠΈΠ΅ ΠΈ обСспСчСниС доступа ΠΊ ΠΈΠ½Ρ„ΠΎΡ€ΠΌΠ°Ρ†ΠΈΠΈ, ΡΡƒΡ‰Π΅ΡΡ‚Π²ΡƒΡŽΡ‰Π΅ΠΉ ΠΊΠ°ΠΊ Π² ΠΏΠ΅Ρ‡Π°Ρ‚Π½ΠΎΠΉ, Ρ‚Π°ΠΊ ΠΈ элСктронной Ρ„ΠΎΡ€ΠΌΠ΅, Ρ‚Ρ€Π°Π΄ΠΈΡ†ΠΈΠΎΠ½Π½ΠΎ заботятся ΠΎ ΠΏΠΎΠ»Π½ΠΎΡ‚Π΅ ΠΈ достовСрности прСдоставляСмых свСдСний ΠΈ источников. Одним ΠΈΠ· способов достиТСния этого являСтся взаимодСйствиС Π±ΠΈΠ±Π»ΠΈΠΎΡ‚Π΅ΠΊ с ΡƒΡ‡Π΅Π½Ρ‹ΠΌΠΈ Π² области систСматизации, Π²Π΅Ρ€ΠΈΡ„ΠΈΠΊΠ°Ρ†ΠΈΠΈ, сохранСния ΠΈ обСспСчСния доступа ΠΊ ΠΈΠ½Ρ„ΠΎΡ€ΠΌΠ°Ρ†ΠΈΠΈ.Π’ ΡΡ‚Π°Ρ‚ΡŒΠ΅ ΠΏΡ€ΠΎΠ°Π½Π°Π»ΠΈΠ·ΠΈΡ€ΠΎΠ²Π°Π½Π° Ρ€Π°Π±ΠΎΡ‚Π° Π²Ρ‹Π΄Π°ΡŽΡ‰Π΅Π³ΠΎΡΡ российского историка П.А. Зайончковского Π² ГосударствСнной Π±ΠΈΠ±Π»ΠΈΠΎΡ‚Π΅ΠΊΠ΅ Π‘Π‘Π‘Π  ΠΈΠΌ. Π’.И. Π›Π΅Π½ΠΈΠ½Π° (Π“Π‘Π›, Π½Ρ‹Π½Π΅ β€” Российская государствСнная Π±ΠΈΠ±Π»ΠΈΠΎΡ‚Π΅ΠΊΠ°, Π Π“Π‘), Π² Π΄Π΅ΡΡ‚Π΅Π»ΡŒΠ½ΠΎΡΡ‚ΠΈ ΠΊΠΎΡ‚ΠΎΡ€ΠΎΠΉ ΡƒΡ‡Π΅Π½Ρ‹ΠΉ ΠΏΡ€ΠΈΠ½ΠΈΠΌΠ°Π» Π°ΠΊΡ‚ΠΈΠ²Π½ΠΎΠ΅ участиС Π² Ρ‚Π΅Ρ‡Π΅Π½ΠΈΠ΅ Π½Π΅ΡΠΊΠΎΠ»ΡŒΠΊΠΈΡ… дСсятилСтий. Π’ 1944β€”1952 Π³Π³. ΠΎΠ½ Π·Π°Π½ΠΈΠΌΠ°Π» Π΄ΠΎΠ»ΠΆΠ½ΠΎΡΡ‚ΡŒ Π·Π°Π²Π΅Π΄ΡƒΡŽΡ‰Π΅Π³ΠΎ ΠΎΡ‚Π΄Π΅Π»ΠΎΠΌ рукописСй Π“Π‘Π›. Под Π΅Π³ΠΎ руководством Π±Ρ‹Π» ΠΎΡ€Π³Π°Π½ΠΈΠ·ΠΎΠ²Π°Π½ ΡƒΡ‡Π΅Ρ‚ ΠΈ обСспСчСна ΡΠΎΡ…Ρ€Π°Π½Π½ΠΎΡΡ‚ΡŒ ΠΈΠΌΠ΅ΡŽΡ‰ΠΈΡ…ΡΡ ΠΈ вновь ΠΏΠΎΡΡ‚ΡƒΠΏΠ°ΡŽΡ‰ΠΈΡ… Ρ„ΠΎΠ½Π΄ΠΎΠ²; ΡƒΡΠΎΠ²Π΅Ρ€ΡˆΠ΅Π½ΡΡ‚Π²ΠΎΠ²Π°Π½ доступ исслСдоватСлСй ΠΊ ΠΈΠ½Ρ„ΠΎΡ€ΠΌΠ°Ρ†ΠΈΠΈ ΠΎΠ± ΠΈΡ… составС ΠΈ содСрТании; сформирован ΡƒΠ½ΠΈΠΊΠ°Π»ΡŒΠ½Ρ‹ΠΉ ΠΊΠΎΠ»Π»Π΅ΠΊΡ‚ΠΈΠ² сотрудников. Π’ ΡΡ‚Π°Ρ‚ΡŒΠ΅ ΠΏΠΎΠ΄Ρ€ΠΎΠ±Π½ΠΎ описываСтся Π½Π°ΡƒΡ‡Π½ΠΎ-публикационная Π°ΠΊΡ‚ΠΈΠ²Π½ΠΎΡΡ‚ΡŒ ΠΎΡ‚Π΄Π΅Π»Π° Π² этот ΠΏΠ΅Ρ€ΠΈΠΎΠ΄. ΠŸΡ€ΠΎΠ°Π½Π°Π»ΠΈΠ·ΠΈΡ€ΠΎΠ²Π°Π½ Π²ΠΊΠ»Π°Π΄ П.А. Зайончковского Π² Ρ€Π°Π·Ρ€Π°Π±ΠΎΡ‚ΠΊΡƒ ΠΏΡ€ΠΎΠ±Π»Π΅ΠΌΡ‹ библиографичСского обСспСчСния историчСской Π½Π°ΡƒΠΊΠΈ. Π’ 1960β€”1980-Ρ… Π³Π³. ΠΏΠΎΠ΄ Π΅Π³ΠΎ Π½Π°ΡƒΡ‡Π½Ρ‹ΠΌ руководством сотрудниками Π“Π‘Π› ΠΈ Π΄Ρ€ΡƒΠ³ΠΈΡ… ΠΊΡ€ΡƒΠΏΠ½Π΅ΠΉΡˆΠΈΡ… Π±ΠΈΠ±Π»ΠΈΠΎΡ‚Π΅ΠΊ Π±Ρ‹Π»ΠΈ ΠΏΠΎΠ΄Π³ΠΎΡ‚ΠΎΠ²Π»Π΅Π½Ρ‹ ΡƒΠ½ΠΈΠΊΠ°Π»ΡŒΠ½Ρ‹Π΅ издания β€” Β«Π‘ΠΏΡ€Π°Π²ΠΎΡ‡Π½ΠΈΠΊΠΈ ΠΏΠΎ истории Π΄ΠΎΡ€Π΅Π²ΠΎΠ»ΡŽΡ†ΠΈΠΎΠ½Π½ΠΎΠΉ России» ΠΈ ΠΌΠ½ΠΎΠ³ΠΎΡ‚ΠΎΠΌΠ½Ρ‹ΠΉ Π°Π½Π½ΠΎΡ‚ΠΈΡ€ΠΎΠ²Π°Π½Π½Ρ‹ΠΉ ΡƒΠΊΠ°Π·Π°Ρ‚Π΅Π»ΡŒ Β«Π˜ΡΡ‚ΠΎΡ€ΠΈΡ Π΄ΠΎΡ€Π΅Π²ΠΎΠ»ΡŽΡ†ΠΈΠΎΠ½Π½ΠΎΠΉ России Π² Π΄Π½Π΅Π²Π½ΠΈΠΊΠ°Ρ… ΠΈ воспоминаниях». П.А. Зайончковский Π·Π°Π»ΠΎΠΆΠΈΠ» основы ΠΌΠ½ΠΎΠ³ΠΎΠ»Π΅Ρ‚Π½Π΅Π³ΠΎ ΠΈ ΠΏΠΎ сСй дСнь Ρ€Π°Π·Π²ΠΈΠ²Π°ΡŽΡ‰Π΅Π³ΠΎΡΡ ΠΏΡ€ΠΎΠ΅ΠΊΡ‚Π° ΠΏΠΎ созданию рСтроспСктивных Π½Π°ΡƒΡ‡Π½ΠΎ-Π²ΡΠΏΠΎΠΌΠΎΠ³Π°Ρ‚Π΅Π»ΡŒΠ½Ρ‹Ρ… ΡƒΠΊΠ°Π·Π°Ρ‚Π΅Π»Π΅ΠΉ ΠΌΠ΅ΠΌΡƒΠ°Ρ€Π½ΠΎΠΉ Π»ΠΈΡ‚Π΅Ρ€Π°Ρ‚ΡƒΡ€Ρ‹. Π”Π΅ΡΡ‚Π΅Π»ΡŒΠ½ΠΎΡΡ‚ΡŒ П.А. Зайончковского Π² Π“Π‘Π› β€” это Π·Π°ΠΌΠ΅Ρ‡Π°Ρ‚Π΅Π»ΡŒΠ½Ρ‹ΠΉ ΠΏΡ€ΠΈΠΌΠ΅Ρ€ ΡƒΡΠΏΠ΅ΡˆΠ½ΠΎΠ³ΠΎ ΠΈ ΠΏΠ»ΠΎΠ΄ΠΎΡ‚Π²ΠΎΡ€Π½ΠΎΠ³ΠΎ взаимодСйствия ΡƒΡ‡Π΅Π½ΠΎΠ³ΠΎ ΠΈ Π±ΠΈΠ±Π»ΠΈΠΎΡ‚Π΅Ρ‡Π½Ρ‹Ρ… Ρ€Π°Π±ΠΎΡ‚Π½ΠΈΠΊΠΎΠ²

    Π‘ΠΈΠ±Π»ΠΈΠΎΠ³Ρ€Π°Ρ„ΠΈΡ€ΠΎΠ²Π°Π½ΠΈΠ΅ Β«Π»ΠΈΡ‡Π½ΠΎΠΉΒ» истории: Π°Π½Π½ΠΎΡ‚ΠΈΡ€ΠΎΠ²Π°Π½Π½Ρ‹Π΅ библиографичСскиС ΡƒΠΊΠ°Π·Π°Ρ‚Π΅Π»ΠΈ ΠΌΠ΅ΠΌΡƒΠ°Ρ€Π½ΠΎΠΉ Π»ΠΈΡ‚Π΅Ρ€Π°Ρ‚ΡƒΡ€Ρ‹

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    The authors determine the specific features of the memoir literature as the historical source;Β there is given the brief overview of the development stages of the domestic memoir history. ScientificΒ value and practical importance of creating the annotated bibliographic indices of the memoir literature isΒ substantiated. The fundamental system of Russian annotated bibliographic indices of memoir literatureΒ (β€œThe History of Soviet Society in Contemporaries’ Memoirs: 1917β€”1957”, β€œThe History of Pre-revolutionary Russia in Diaries and Memoirs”, β€œRussia and Russian Emigration in Memoirs and Diaries”, β€œSoviet Society in Memoirs and Diaries”) is characterized. The analysis of authors, professional experience in creating the annotated bibliographic index of memoir literature β€œThe History of Russia and USSR in Memoirs and Diaries” is given. The authors formulate the main principles, used in the process of memoir selection. The principles, such as the form of publication, the β€œpurity of genre”, the authenticity, scientific credibility, the artistic and historical value, are discussed.ΠžΠΏΡ€Π΅Π΄Π΅Π»ΡΡŽΡ‚ΡΡ особСнности ΠΌΠ΅ΠΌΡƒΠ°Ρ€Π½ΠΎΠΉ Π»ΠΈΡ‚Π΅Ρ€Π°Ρ‚ΡƒΡ€Ρ‹ ΠΊΠ°ΠΊ историчСского источника, даСтся краткий ΠΎΠ±Π·ΠΎΡ€ развития отСчСствСнной мСмуаристики. ΠžΠ±ΠΎΡΠ½ΠΎΠ²Ρ‹Π²Π°Π΅Ρ‚ΡΡ Π½Π°ΡƒΡ‡Π½ΠΎΠ΅ Π·Π½Π°Ρ‡Π΅Π½ΠΈΠ΅ ΠΈ практичСская Π½Π΅ΠΎΠ±Ρ…ΠΎΠ΄ΠΈΠΌΠΎΡΡ‚ΡŒ создания Π°Π½Π½ΠΎΡ‚ΠΈΡ€ΠΎΠ²Π°Π½Π½Ρ‹Ρ… библиографичСских ΡƒΠΊΠ°Π·Π°Ρ‚Π΅Π»Π΅ΠΉ ΠΌΠ΅ΠΌΡƒΠ°Ρ€Π½ΠΎΠΉ Π»ΠΈΡ‚Π΅Ρ€Π°Ρ‚ΡƒΡ€Ρ‹.Β Π₯Π°Ρ€Π°ΠΊΡ‚Π΅Ρ€ΠΈΠ·ΡƒΡŽΡ‚ΡΡ Ρ„ΡƒΠ½Π΄Π°ΠΌΠ΅Π½Ρ‚Π°Π»ΡŒΠ½Ρ‹Π΅ ΡƒΠΊΠ°Π·Π°Ρ‚Π΅Π»ΠΈ ΠΏΠΎ заявлСнной Ρ‚Π΅ΠΌΠ°Ρ‚ΠΈΠΊΠ΅: Β«Π˜ΡΡ‚ΠΎΡ€ΠΈΡ совСтского общСства в воспоминаниях соврСмСнников: 1917β€”1957Β», Β«Π˜ΡΡ‚ΠΎΡ€ΠΈΡ Π΄ΠΎΡ€Π΅Π²ΠΎΠ»ΡŽΡ†ΠΈΠΎΠ½Π½ΠΎΠΉ России Π² Π΄Π½Π΅Π²Π½ΠΈΠΊΠ°Ρ… ΠΈ воспоминаниях», «Россия ΠΈ российская эмиграция Π² воспоминаниях ΠΈ Π΄Π½Π΅Π²Π½ΠΈΠΊΠ°Ρ…Β», «БовСтскоС общСство в воспоминаниях ΠΈ Π΄Π½Π΅Π²Π½ΠΈΠΊΠ°Ρ…Β». АнализируСтся практичСский ΠΎΠΏΡ‹Ρ‚ Π°Π²Ρ‚ΠΎΡ€ΠΎΠ² Π² создании библиографичСского указатСля Β«Π˜ΡΡ‚ΠΎΡ€ΠΈΡ России ΠΈ Π‘Π‘Π‘Π  Π² воспоминаниях ΠΈ Π΄Π½Π΅Π²Π½ΠΈΠΊΠ°Ρ…Β». Π€ΠΎΡ€ΠΌΡƒΠ»ΠΈΡ€ΡƒΡŽΡ‚ΡΡ ΠΏΡ€ΠΈΠ½Ρ†ΠΈΠΏΡ‹ ΠΎΡ‚Π±ΠΎΡ€Π° источников для Π²ΠΊΠ»ΡŽΡ‡Π΅Π½ΠΈΡ Π² ΡƒΠΊΠ°Π·Π°Ρ‚Π΅Π»ΠΈ: Ρ„ΠΎΡ€ΠΌΠ° ΠΏΡƒΠ±Π»ΠΈΠΊΠ°Ρ†ΠΈΠΈ, чистота ΠΆΠ°Π½Ρ€Π°, ΠΏΠΎΠ΄Π»ΠΈΠ½Π½ΠΎΡΡ‚ΡŒ,Β Π΄ΠΎΡΡ‚ΠΎΠ²Π΅Ρ€Π½ΠΎΡΡ‚ΡŒ, худоТСствСнная ΠΈ историчСская Ρ†Π΅Π½Π½ΠΎΡΡ‚ΡŒ источника

    Fatty acids composition of Bacillus subtilis ONU551 lipids

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    The aim of the study was to determine the cellular lipids fatty acid composition for identification of the Bacillus subtilis ONU551 strain bacteria, which is a phenol destructor. Fatty acids analysis of B. subtilis ONU551 strain was performed using an automatic system for microorganisms’ identification MIDI Sherlock (MIDI, USA) based on gas chromatograph Agilent 7890. Chromatograms analysis showed that the fatty acid spectrum of the strain B. subtilis ONU551 consisted predominately of branched structural isomers of saturated acids: 13-methyltetradecanoic (15:0 iso; 34.72%) and 12-methyltetradecanoic (15:0 anteiso; 33.72%) acids. The total content of the branched saturated fatty acids was 88.16% – 14:0 iso (0.52%), 15:0 iso (34.72%), 15:0 anteiso (33.72%), 16:0 iso (1.85%), 17:0 iso (7.11%), 17:0 anteiso (10.24%). The saturated fatty acids of the normal structure were also detected – 12:0 (0.36%), 14:0 (0.28%), 16:0 (1.30%). No 2- and 3-hydroxy acids and no cyclic fatty acids were detected in the fatty acid profile of B. subtilis ONU551 strain. Unsaturated fatty acid isomers – 15:1 w5c (1.85%), 16:1 w11c (1.21%), 16:1 w7c alcohol (1.08%), 17:1 iso w10c (3.18%), βˆ‘17:1 iso I/anteiso B (2.57%) were shown to be the distinctive biomarkers of the B. subtilis ONU551 strain. According to the fatty acid profile analysis with MIDI Sherlock system, the studied strain was identified as Bacillus subtilis with high level of similarity index (0.563)

    Cellular fatty acid composition of Aeromonas genus – destructor of aromatic xenobiotics

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    The aim of this study was a determination of the fatty acid composition of cellular lipids and identification of the strains, isolated from the wastewater of pharmaceutical production, – the destructor of aromatic xenobiotics. The phenotypic characteristics and cellular fatty acid (FA) composition confirmed the strain belonging to the Aeromonas ichthiosmia with the similarity index of library data MIDI Sherlock – 0.564. Analysis of the cellular FA composition of the strain Aeromonas ichthiosmia ONU552 was carried out using the MIDI Sherlock microorganism identification system based on the gas chromatograph Agilent 7890. Chromatographic analysis showed that the fatty acid profile of the strain Aeromonas ichthiosmia ONU552 contains 26 fatty acids with the total number of carbon atoms from 10 to 18. 85.27% of saturated and unsaturated fatty acids had unbranched structure. The total content of unsaturated fatty acids – 16:1 w7c/16:1 w6c, 18:1 w7c, 16:1 w7c alcohol, 17:1 w8c, 17:1 w6c, 16:1 w5c, was 50% of the total fatty acid pool. Less than 1.5% branched fatty acids were predominantly in the iso form: 13:0 iso (0.20%); 15:0 iso (0.97%); 17:1 iso w9c (1.35%), 17:0 iso (1.49%); in the anteiso form, only one acid 17:0 (0.27%) was identified. It was shown that the characterisΒ­tic of the fatty acid composition of the strain Aeromonas ichthiosmia ONU552 – the destructor of aromatic xenobioΒ­tics, was the presence of hydroxyacids 12:0 3OH, 15:0 3OH, 15:0 iso 3OH and dominance of hexadecanoic (16:0) and hexadecenoic (16:1 w7c/16:1 w6c) of fatty acids

    Intracellular S1P Generation Is Essential for S1P-Induced Motility of Human Lung Endothelial Cells: Role of Sphingosine Kinase 1 and S1P Lyase

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    Earlier we have shown that extracellular sphingosine-1-phosphate (S1P) induces migration of human pulmonary artery endothelial cells (HPAECs) through the activation of S1P(1) receptor, PKCΞ΅, and PLD2-PKCΞΆ-Rac1 signaling cascade. As endothelial cells generate intracellular S1P, here we have investigated the role of sphingosine kinases (SphKs) and S1P lyase (S1PL), that regulate intracellular S1P accumulation, in HPAEC motility

    Π”Π˜ΠΠΠœΠ˜ΠšΠ ΠŸΠžΠšΠΠ—ΠΠ’Π•Π›Π•Π™ Π‘Π•Π›ΠšΠžΠ’ΠžΠ“Πž ΠžΠ‘ΠœΠ•ΠΠ Π£ ΠšΠžΠ ΠžΠ’ ПРИ ΠŸΠ Π˜ΠœΠ•ΠΠ•ΠΠ˜Π˜ ΠŸΠ ΠžΠ‘Π˜ΠžΠ’Π˜Π§Π•Π‘ΠšΠžΠ“Πž ΠŸΠ Π•ΠŸΠ ΠΠ’Π Π’Π•Π’ΠžΠœ 1 НА ΠžΠ‘ΠΠžΠ’Π• ΠΠŸΠΠ’ΠžΠ“Π•ΠΠ«Π₯ Π‘ΠΠ¦Π˜Π›Π›

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    The researchers investigated the effect of new probiotic Vetom 1 based on apathogenic bacilli on the biochemical parameters of blood serum, exactly protein, albumins and urea in lactating cows. The experiment was carried out at OOO β€œUchkhoz Tulinskoe”. According to the principle of pair-analogues, the researchers arranged a control group and two experimental groups of cows; each group contained 7 cows. The cows of the 1st experimental group were fed with probiotic Vetom 1 dosed 50 mg/kg once a day in the morning before milking; They received Vetom 1 every day during 5 days, then in a day during a month. The cows from the 2nd experimental group received probiotic Vetom 1 dosed 50 mg/kg once a day in the morning before milking, every day during 30 days. The application of the specimen contributed to a decrease in the concentration of total protein in the blood serum within the physiological norm. The aftereffect caused by Vetom 1 when the specimen was applied 5 days every day, then in a day during a month, the authors observed an increase in the concentration of total protein in the blood serum above the physiological standard on the 180 day of application. The similar effect was not observed when Vetom 1 was applied daily. When using Vetom 1, the authors observed a slight increase in the concentration of albumins in the blood serum, both during the period of application and up to 180 days. The specimen contributes to less prominent increase in the concentration of urea in the blood serum in comparison with the control group. Changes in concentrations of albumins and urea occurred within the physiological norm.Π˜Π·ΡƒΡ‡Π°Π»ΠΎΡΡŒ дСйствиС Π½ΠΎΠ²ΠΎΠ³ΠΎ пробиотичСского ΠΏΡ€Π΅ΠΏΠ°Ρ€Π°Ρ‚Π° Π’Π΅Ρ‚ΠΎΠΌ 1 Π½Π° основС Π°ΠΏΠ°Ρ‚ΠΎΠ³Π΅Π½Π½Ρ‹Ρ… Π±Π°Ρ†ΠΈΠ»Π» Π½Π° биохимичСскиС ΠΏΠΎΠΊΠ°Π·Π°Ρ‚Π΅Π»ΠΈ сыворотки ΠΊΡ€ΠΎΠ²ΠΈ, Π° ΠΈΠΌΠ΅Π½Π½ΠΎ Π±Π΅Π»ΠΊΠ°, Π°Π»ΡŒΠ±ΡƒΠΌΠΈΠ½ΠΎΠ² ΠΈ ΠΌΠΎΡ‡Π΅Π²ΠΈΠ½Ρ‹, Ρƒ Π»Π°ΠΊΡ‚ΠΈΡ€ΡƒΡŽΡ‰ΠΈΡ… ΠΊΠΎΡ€ΠΎΠ². ΠžΠΏΡ‹Ρ‚ ΠΏΡ€ΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΈ Π² ООО Β«Π£Ρ‡Ρ…ΠΎΠ· ВулинскоС». По ΠΏΡ€ΠΈΠ½Ρ†ΠΈΠΏΡƒ ΠΏΠ°Ρ€-Π°Π½Π°Π»ΠΎΠ³ΠΎΠ² Π±Ρ‹Π»ΠΈ сформированы ΠΎΠ΄Π½Π° ΠΊΠΎΠ½Ρ‚Ρ€ΠΎΠ»ΡŒΠ½Π°Ρ ΠΈ Π΄Π²Π΅ ΠΎΠΏΡ‹Ρ‚Π½Ρ‹Π΅ Π³Ρ€ΡƒΠΏΠΏΡ‹ ΠΊΠΎΡ€ΠΎΠ², Π² ΠΊΠ°ΠΆΠ΄ΠΎΠΉ ΠΏΠΎ 7 ΠΆΠΈΠ²ΠΎΡ‚Π½Ρ‹Ρ…. ΠšΠΎΡ€ΠΎΠ²Π°ΠΌ 1-ΠΉ ΠΎΠΏΡ‹Ρ‚Π½ΠΎΠΉ Π³Ρ€ΡƒΠΏΠΏΡ‹ Π·Π°Π΄Π°Π²Π°Π»ΠΈ пробиотичСский ΠΏΡ€Π΅ΠΏΠ°Ρ€Π°Ρ‚ Π’Π΅Ρ‚ΠΎΠΌ 1 Π² Π΄ΠΎΠ·Π΅ 50 ΠΌΠ³/ΠΊΠ³ Ρ€Π°Π· Π² сутки, ΡƒΡ‚Ρ€ΠΎΠΌ ΠΏΠ΅Ρ€Π΅Π΄ Π΄ΠΎΠΉΠΊΠΎΠΉ, 5 суток Π΅ΠΆΠ΅Π΄Π½Π΅Π²Π½ΠΎ, Π·Π°Ρ‚Π΅ΠΌ Ρ‡Π΅Ρ€Π΅Π· сутки Π² Ρ‚Π΅Ρ‡Π΅Π½ΠΈΠ΅ мСсяца. ΠšΠΎΡ€ΠΎΠ²Π°ΠΌ 2-ΠΉ ΠΎΠΏΡ‹Ρ‚Π½ΠΎΠΉ Π³Ρ€ΡƒΠΏΠΏΡ‹ примСняли пробиотичСский ΠΏΡ€Π΅ΠΏΠ°Ρ€Π°Ρ‚ Π’Π΅Ρ‚ΠΎΠΌ 1 Π² Π΄ΠΎΠ·Π΅ 50 ΠΌΠ³/ΠΊΠ³ Ρ€Π°Π· Π² сутки, ΡƒΡ‚Ρ€ΠΎΠΌ ΠΏΠ΅Ρ€Π΅Π΄ Π΄ΠΎΠΉΠΊΠΎΠΉ, Π΅ΠΆΠ΅Π΄Π½Π΅Π²Π½ΠΎ Π² Ρ‚Π΅Ρ‡Π΅Π½ΠΈΠ΅ мСсяца. ΠŸΡ€ΠΈ ΠΏΡ€ΠΈΠΌΠ΅Π½Π΅Π½ΠΈΠΈ ΠΈΠ·ΡƒΡ‡Π°Π΅ΠΌΠΎΠ³ΠΎ ΠΏΡ€Π΅ΠΏΠ°Ρ€Π°Ρ‚Π° происходило ΠΏΠΎΠ½ΠΈΠΆΠ΅Π½ΠΈΠ΅ ΠΊΠΎΠ½Ρ†Π΅Π½Ρ‚Ρ€Π°Ρ†ΠΈΠΈ ΠΎΠ±Ρ‰Π΅Π³ΠΎ Π±Π΅Π»ΠΊΠ° Π² сывороткС ΠΊΡ€ΠΎΠ²ΠΈ Π² ΠΏΡ€Π΅Π΄Π΅Π»Π°Ρ… физиологичСской Π½ΠΎΡ€ΠΌΡ‹. Π’ ΠΏΠ΅Ρ€ΠΈΠΎΠ΄ послСдСйствия ΠΏΡ€ΠΈ ΠΏΡ€ΠΈΠΌΠ΅Π½Π΅Π½ΠΈΠΈ Π’Π΅Ρ‚ΠΎΠΌΠ° 1 послС 5 суток Π΅ΠΆΠ΅Π΄Π½Π΅Π²Π½ΠΎ, Π·Π°Ρ‚Π΅ΠΌ Ρ‡Π΅Ρ€Π΅Π· сутки Π² Ρ‚Π΅Ρ‡Π΅Π½ΠΈΠ΅ мСсяца Π½Π° 180-Π΅ сутки послС Π½Π°Ρ‡Π°Π»Π° примСнСния ΠΏΡ€Π΅ΠΏΠ°Ρ€Π°Ρ‚Π° рСгистрировали ΠΏΠΎΠ²Ρ‹ΡˆΠ΅Π½ΠΈΠ΅ ΠΊΠΎΠ½Ρ†Π΅Π½Ρ‚Ρ€Π°Ρ†ΠΈΠΈ ΠΎΠ±Ρ‰Π΅Π³ΠΎ Π±Π΅Π»ΠΊΠ° Π² сывороткС ΠΊΡ€ΠΎΠ²ΠΈ Π²Ρ‹ΡˆΠ΅ физиологичСской Π½ΠΎΡ€ΠΌΡ‹. ΠŸΡ€ΠΈ ΠΏΡ€ΠΈΠΌΠ΅Π½Π΅Π½ΠΈΠΈ Π’Π΅Ρ‚ΠΎΠΌΠ° 1 Π΅ΠΆΠ΅Π΄Π½Π΅Π²Π½ΠΎ Π°Π½Π°Π»ΠΎΠ³ΠΈΡ‡Π½Ρ‹ΠΉ эффСкт Π½Π΅ наблюдали. ΠžΡ‚ΠΌΠ΅Ρ‡Π°Π»ΠΈ лишь Π½Π΅Π·Π½Π°Ρ‡ΠΈΡ‚Π΅Π»ΡŒΠ½ΠΎΠ΅ ΠΏΠΎΠ²Ρ‹ΡˆΠ΅Π½ΠΈΠ΅ ΠΊΠΎΠ½Ρ†Π΅Π½Ρ‚Ρ€Π°Ρ†ΠΈΠΈ Π°Π»ΡŒΠ±ΡƒΠΌΠΈΠ½ΠΎΠ² Π² сывороткС ΠΊΡ€ΠΎΠ²ΠΈ ΠΊΠ°ΠΊ Π² ΠΏΠ΅Ρ€ΠΈΠΎΠ΄ примСнСния, Ρ‚Π°ΠΊ ΠΈ Π΄ΠΎ 180-Ρ… суток послС Π½Π°Ρ‡Π°Π»Π° примСнСния ΠΏΡ€Π΅ΠΏΠ°Ρ€Π°Ρ‚Π°. ΠŸΡ€ΠΈ ΠΏΡ€ΠΈΠΌΠ΅Π½Π΅Π½ΠΈΠΈ ΠΈΠ·ΡƒΡ‡Π°Π΅ΠΌΠΎΠ³ΠΎ ΠΏΡ€Π΅ΠΏΠ°Ρ€Π°Ρ‚Π° происходит ΠΌΠ΅Π½Π΅Π΅ Π²Ρ‹Ρ€Π°ΠΆΠ΅Π½Π½ΠΎΠ΅ ΠΏΠΎΠ²Ρ‹ΡˆΠ΅Π½ΠΈΠ΅ ΠΊΠΎΠ½Ρ†Π΅Π½Ρ‚Ρ€Π°Ρ†ΠΈΠΈ ΠΌΠΎΡ‡Π΅Π²ΠΈΠ½Ρ‹ Π² сывороткС ΠΊΡ€ΠΎΠ²ΠΈ ΠΎΡ‚Π½ΠΎΡΠΈΡ‚Π΅Π»ΡŒΠ½ΠΎ Π°Π½Π°Π»ΠΎΠ³ΠΎΠ² ΠΈΠ· ΠΊΠΎΠ½Ρ‚Ρ€ΠΎΠ»ΡŒΠ½ΠΎΠΉ Π³Ρ€ΡƒΠΏΠΏΡ‹. ИзмСнСниС ΠΊΠΎΠ½Ρ†Π΅Π½Ρ‚Ρ€Π°Ρ†ΠΈΠΉ Π°Π»ΡŒΠ±ΡƒΠΌΠΈΠ½ΠΎΠ² ΠΈ ΠΌΠΎΡ‡Π΅Π²ΠΈΠ½Ρ‹ происходило Π² ΠΏΡ€Π΅Π΄Π΅Π»Π°Ρ… физиологичСской Π½ΠΎΡ€ΠΌΡ‹

    Pectobacterium atrosepticum exopolysaccharides: Identification, molecular structure, formation under stress and in planta conditions

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    Β© The Author 2017. Published by Oxford University Press. All rights reserved. In the present study, we identified exopolysaccharides of the harmful phytopathogenic bacterium Pectobacterium atrosepticum SCRI1043 and characterized the molecular structure of these polymers. The synthesis of the target polysaccharides was shown to be induced under starvation conditions. Moreover, intensive accumulation of exopolysaccharides occurred during the colonization by bacteria of the xylem vessels of infected plants, where microorganisms formed specific 3D "multicellular" structures-bacterial emboli. Thus, the identified polymers are likely to be involved in the adaptation and virulence of bacteria of Pectobacterium genus
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