Optical design for the narrow field infrared adaptive optics system (NFIRAOS) petite on the thirty meter telescope

We describe an exploratory optical design for the Narrow Field InfraRed Adaptive Optics (AO) System (NFIRAOS) Petite, a proposed adaptive optics system for the Thirty Meter Telescope Project. NFIRAOS will feed infrared spectrograph and wide-field imaging instruments with a diffraction limited beam. The adaptive optics system will require multi-guidestar tomographic wavefront sensing (WFS) and multi-conjugate AO correction. The NFIRAOS Petite design specifications include two small 60 mm diameter deformable mirrors (DM's) used in a woofer/tweeter or multiconjugate arrangement. At least one DM would be a micro-electromechanical system (MEMS) DM. The AO system would correct a 10 to 30 arcsec diameter science field as well as laser guide stars (LGS's) located within a 60 arcsec diameter field and low-order or tip/tilt natural guide stars (NGS's) within a 60 arcsec diameter field. The WFS's are located downstream of the DM's so that they can be operated in true closed-loop, which is not necessarily a given in extremely large telescope adaptive optics design. The WFS's include adjustable corrector elements which correct the static aberrations of the AO relay due to field position and LGS distance height

Structural stability of adenylate kinase from the sulfate-reducing bacteria Desulfovibrio gigas

Biophysical Chemistry 110 (2004) 83–92A novel adenylate kinase (AK) has recently been purified from Desulfovibrio gigas and characterized as a Co2+/Zn2+-containing enzyme: this is an unusual characteristic for AKs from Gram-negative bacteria, in which these enzymes are normally devoid of metals. Here, we studied the conformational stability of holo- and apo-AK as a function of temperature by differential scanning calorimetry (DSC), circular dichroism (CD), and intrinsic fluorescence spectroscopy. The thermal unfolding of AK is a cooperative two-state process, and is sufficiently reversible in the 9–11 pH range, that can be correctly interpreted in terms of a simple two-state thermodynamic model. The spectral parameters as monitored by ellipticity changes in the CD spectra of the enzyme as well as the decrease in tryptophan intensity emission upon heating were seen to be good complements to the highly sensitive but integral DSC-method.supported in part by the Fundação para a Ciência e a Tecnologia, Portugal, fellowships BD/13775/97 to OYG, BPD/3518/00 to SAB, SFRH/BD/1067/2000 to DGP, and NATO Scientific Programme Fellowships for Spain, call 2002 to GGZ

Characterizing the Adaptive Optics Off-Axis Point-Spread Function. II. Methods for Use in Laser Guide Star Observations

Most current astronomical adaptive optics (AO) systems rely on the availability of a bright star to measure the distortion of the incoming wavefront. Replacing the guide star with an artificial laser beacon alleviates this dependency on bright stars and therefore increases sky coverage, but it does not eliminate another serious problem for AO observations. This is the issue of PSF variation with time and field position near the guide star. In fact, because a natural guide star is still necessary for correction of the low-order phase error, characterization of laser guide star (LGS) AO PSF spatial variation is more complicated than for a natural guide star alone. We discuss six methods for characterizing LGS AO PSF variation that can potentially improve the determination of the PSF away from the laser spot, that is, off-axis. Calibration images of dense star fields are used to determine the change in PSF variation with field position. This is augmented by AO system telemetry and simple computer simulations to determine a more accurate off-axis PSF. We report on tests of the methods using the laser AO system on the Lick Observatory Shane Telescope. [Abstract truncated.]Comment: 31 pages, 5 figures, accepted by PAS

Optical design for the narrow field infrared adaptive optics system (NFIRAOS) petite on the thirty meter telescope

We describe an exploratory optical design for the Narrow Field InfraRed Adaptive Optics (AO) System (NFIRAOS) Petite, a proposed adaptive optics system for the Thirty Meter Telescope Project. NFIRAOS will feed infrared spectrograph and wide-field imaging instruments with a diffraction limited beam. The adaptive optics system will require multi-guidestar tomographic wavefront sensing (WFS) and multi-conjugate AO correction. The NFIRAOS Petite design specifications include two small 60 mm diameter deformable mirrors (DM's) used in a woofer/tweeter or multiconjugate arrangement. At least one DM would be a micro-electromechanical system (MEMS) DM. The AO system would correct a 10 to 30 arcsec diameter science field as well as laser guide stars (LGS's) located within a 60 arcsec diameter field and low-order or tip/tilt natural guide stars (NGS's) within a 60 arcsec diameter field. The WFS's are located downstream of the DM's so that they can be operated in true closed-loop, which is not necessarily a given in extremely large telescope adaptive optics design. The WFS's include adjustable corrector elements which correct the static aberrations of the AO relay due to field position and LGS distance height

Crystal structure of the zinc-, cobalt-, and iron-containing adenylate kinase from Desulfovibrio gigas: a novel metal-containing adenylate kinase from Gram-negative bacteria

J Biol Inorg Chem (2011) 16:51–61 DOI 10.1007/s00775-010-0700-8Adenylate kinases (AK) from Gram-negative bacteria are generally devoid of metal ions in their LID domain. However, three metal ions, zinc, cobalt, and iron, have been found in AK from Gram-negative bacteria. Crystal structures of substrate-free AK from Desulfovibrio gigas with three different metal ions (Zn2+, Zn-AK; Co2+, Co-AK; and Fe2+, Fe-AK) bound in its LID domain have been determined by X-ray crystallography to resolutions 1.8, 2.0, and 3.0 Å, respectively. The zinc and iron forms of the enzyme were crystallized in space group I222, whereas the cobalt-form crystals were C2. The presence of the metals was confirmed by calculation of anomalous difference maps and by X-ray fluorescence scans. The work presented here is the first report of a structure of a metal-containing AK from a Gram-negative bacterium. The native enzyme was crystallized, and only zinc was detected in the LID domain. Co-AK and Fe-AK were obtained by overexpressing the protein in Escherichia coli. Zn-AK and Fe-AK crystallized as monomers in the asymmetric unit, whereas Co-AK crystallized as a dimer. Nevertheless, all three crystal structures are very similar to each other, with the same LID domain topology, the only change being the presence of the different metal atoms. In the absence of any substrate, the LID domain of all holoforms of AK was present in a fully open conformational state. Normal mode analysis was performed to predict fluctuations of the LID domain along the catalytic pathway

Stellar Companions to Stars with Planets

A combination of high-resolution and wide-field imaging reveals two binary stars and one triple star system among the sample of the first 11 stars with planets detected by radial velocity variations. High resolution speckle or adaptive optics (AO) data probe subarcsecond scales down to the diffraction limit of the Keck 10-m or Lick 3-m, and direct images or AO images are sensitive to a wider field, extending to 10" or 38", depending upon the camera. One of the binary systems -- HD 114762 -- was not previously known to be a spatially resolved multiple system; additional data taken with the combination of Keck adaptive optics and NIRSPEC are used to characterize the new companion. The second binary system -- Tau Boo -- was a known multiple with two conflicting orbital solutions; the current data will help constrain the discrepant estimates of periastron time and separation. Another target -- 16 Cyg B -- was a known common proper motion binary, but the current data resolve a new third component, close to the wide companion 16 Cyg A. Both the HD 114762 and 16 Cyg B systems harbor planets in eccentric orbits, while the Tau Boo binary contains an extremely close planet in a tidally-circularized orbit. Although the sample is currently small, the proportion of binary systems is comparable to that measured in the field over a similar separation range. Incorporating the null result from another companion search project lowers the overall fraction of planets in binary systems, but the detections in our survey reveal that planets can form in binaries separated by less than 50 AU.Comment: 5 Tables, 16 Figures. ApJ, accepte

The Gaia-ESO Survey: Spectroscopic-asteroseismic analysis of K2 stars in Gaia-ESO

The extensive stellar spectroscopic datasets that are available for studies in Galactic Archeaology thanks to, for example, the Gaia-ESO Survey, now benefit from having a significant number of targets that overlap with asteroseismology projects such as Kepler, K2 and CoRoT. Combining the measurements from spectroscopy and asteroseismology allows us to attain greater accuracy with regard to the stellar parameters needed to characterise the stellar populations of the Milky Way. The aim of this Gaia-ESO Survey special project is to produce a catalogue of self-consistent stellar parameters by combining measurements from high-resolution spectroscopy and precision asteroseismology. We carried out an iterative analysis of 90 K2@Gaia-ESO red giants. The spectroscopic values of Teff were used as input in the seismic analysis to obtain log(g) values. The seismic estimates of log(g) were then used to re-determine the spectroscopic values of Teff and [Fe/H]. Only one iteration was required to obtain parameters that are in good agreement for both methods and thus, to obtain the final stellar parameters. A detailed analysis of outliers was carried out to ensure a robust determination of the parameters. The results were then combined with Gaia DR2 data to compare the seismic log(g) with a parallax-based log(g) and to investigate instances of variations in the velocity and possible binaries within the dataset. This analysis produced a high-quality catalogue of stellar parameters for 90 red giant stars observed by both K2 and Gaia-ESO that were determined through iterations between spectroscopy and asteroseismology. We compared the seismic gravities with those based on Gaia parallaxes to find an offset which is similar to other studies that have used asteroseismology. Our catalogue also includes spectroscopic chemical abundances and radial velocities, as well as indicators for possible binary detections

Physician supply forecast: better than peering in a crystal ball?

<p>Abstract</p> <p>Background</p> <p>Anticipating physician supply to tackle future health challenges is a crucial but complex task for policy planners. A number of forecasting tools are available, but the methods, advantages and shortcomings of such tools are not straightforward and not always well appraised. Therefore this paper had two objectives: to present a typology of existing forecasting approaches and to analyse the methodology-related issues.</p> <p>Methods</p> <p>A literature review was carried out in electronic databases Medline-Ovid, Embase and ERIC. Concrete examples of planning experiences in various countries were analysed.</p> <p>Results</p> <p>Four main forecasting approaches were identified. The supply projection approach defines the necessary inflow to maintain or to reach in the future an arbitrary predefined level of service offer. The demand-based approach estimates the quantity of health care services used by the population in the future to project physician requirements. The needs-based approach involves defining and predicting health care deficits so that they can be addressed by an adequate workforce. Benchmarking health systems with similar populations and health profiles is the last approach. These different methods can be combined to perform a gap analysis. The methodological challenges of such projections are numerous: most often static models are used and their uncertainty is not assessed; valid and comprehensive data to feed into the models are often lacking; and a rapidly evolving environment affects the likelihood of projection scenarios. As a result, the internal and external validity of the projections included in our review appeared limited.</p> <p>Conclusion</p> <p>There is no single accepted approach to forecasting physician requirements. The value of projections lies in their utility in identifying the current and emerging trends to which policy-makers need to respond. A genuine gap analysis, an effective monitoring of key parameters and comprehensive workforce planning are key elements to improving the usefulness of physician supply projections.</p

Mice have a transcribed L-threonine aldolase/GLY1 gene, but the human GLY1 gene is a non-processed pseudogene

BACKGROUND: There are three pathways of L-threonine catabolism. The enzyme L-threonine aldolase (TA) has been shown to catalyse the conversion of L-threonine to yield glycine and acetaldehyde in bacteria, fungi and plants. Low levels of TA enzymatic activity have been found in vertebrates. It has been suggested that any detectable activity is due to serine hydroxymethyltransferase and that mammals lack a genuine threonine aldolase. RESULTS: The 7-exon murine L-threonine aldolase gene (GLY1) is located on chromosome 11, spanning 5.6 kb. The cDNA encodes a 400-residue protein. The protein has 81% similarity with the bacterium Thermotoga maritima TA. Almost all known functional residues are conserved between the two proteins including Lys242 that forms a Schiff-base with the cofactor, pyridoxal-5'-phosphate. The human TA gene is located at 17q25. It contains two single nucleotide deletions, in exons 4 and 7, which cause frame-shifts and a premature in-frame stop codon towards the carboxy-terminal. Expression of human TA mRNA was undetectable by RT-PCR. In mice, TA mRNA was found at low levels in a range of adult tissues, being highest in prostate, heart and liver. In contrast, serine/threonine dehydratase, another enzyme that catabolises L-threonine, is expressed very highly only in the liver. Serine dehydratase-like 1, also was most abundant in the liver. In whole mouse embryos TA mRNA expression was low prior to E-15 increasing more than four-fold by E-17. CONCLUSION: Mice, the western-clawed frog and the zebrafish have transcribed threonine aldolase/GLY1 genes, but the human homolog is a non-transcribed pseudogene. Serine dehydratase-like 1 is a putative L-threonine catabolising enzyme