16,222 research outputs found
Islets of Langerhans Are Protected from Inflammatory Cell Recruitment during Reperfusion of Rat Pancreas Grafts
Background: Ischemia/reperfusion (I/R) injury plays a pivotal role in the development of graft pancreatitis, with ischemia time representing one of its crucial factors. However, it is unclear, whether exocrine and endocrine tissue experience similar inflammatory responses during pancreas transplantation (PTx). This study evaluated inflammatory susceptibilities of islets of Langerhans (ILH) and exocrine tissue after different preservation periods during early reperfusion. Methods: PTx was performed in rats following 2 h (2h-I) or 18 h (18h-I) preservation. Leukocyte-endothelial cell interactions (LEI) were analyzed in venules of acinar tissue and ILH in vivo over 2 h reperfusion. Nontransplanted animals served as controls. Tissue samples were analyzed by histomorphometry. Results: In exocrine venules leukocyte rolling predominated in the 2h-I group. In the 18h-I group, additionally, high numbers of adherent leukocytes were found. Histology revealed significant edema formation and leukocyte extravasation in the 18h-I group. Notably, LEI in postcapillary venules of ILH were significantly lower. Leukocyte rolling was only moderately enhanced and few leukocytes were found adherent. Histology revealed minor leukocyte extravasation. Conclusion: Ischemia time contributes decisively to the extent of the I/R-injury in PTx. However, ILH have a significantly lower susceptibility towards I/R, even when inflammatory reactions in adjacent exocrine tissue are evident. Copyright (C) 2010 S. Karger AG, Base
Summary of random vibration prediction procedures
Summary of random vibration prediction procedures for aerospace vehicles, with bibliograph
X-ray properties of an Unbiased Hard X-ray Detected Sample of AGN
The SWIFT gamma ray observatory's Burst Alert Telescope (BAT) has detected a
sample of active galactic nuclei (AGN) based solely on their hard X-ray flux
(14-195 keV). In this paper, we present for the first time {\it XMM-Newton}
X-ray spectra for 22 BAT AGNs with no previously analyzed X-ray spectra. If our
sources are a representative sample of the BAT AGN, as we claim, our results
present for the first time global X-ray properties of an unbiased towards
absorption (n), AGN
sample. We find 9/22 low absorption (n cm), simple power
law model sources, where 4 of these sources have a statistically significant
soft component. Among these sources, we find the presence of a warm absorber
statistically significant for only one Seyfert 1 source, contrasting with the
ASCA results of \citet{rey97} and \citet{geo98}, who find signatures of warm
absorption in half or more of their Seyfert 1 samples at similar redshifts.
Additionally, the remaining sources (14/22) have more complex spectra, well-fit
by an absorbed power law at keV. Five of the complex sources are
classified as Compton-thick candidates. Further, we find four more sources with
properties consistent with the hidden/buried AGN reported by Ueda {\it et al.}
(2007). Finally, we include a comparison of the {\it XMM-Newton} EPIC spectra
with available SWIFT X-ray Telescope (XRT) observations. From these
comparisons, we find 6/16 sources with varying column densities, 6/16 sources
with varying power law indices, and 13/16 sources with varying fluxes, over
periods of hours to months. Flux and power law index are correlated for objects
where both parameters vary.Comment: 39 pages, 16 figures, accepted to Ap
A novel technique for selective NF-kappa B inhibition in Kupffer cells: contrary effects in fulminant hepatitis and ischaemia-reperfusion.
Background and aims: The transcription factor nuclear
factor kappa B (NF-kB) has risen as a promising target for
anti-inflammatory therapeutics. In the liver, however, NFkB
inhibition mediates both damaging and protective
effects. The outcome is deemed to depend on the liver
cell type addressed. Recent gene knock-out studies
focused on the role of NF-kB in hepatocytes, whereas the
role of NF-kB in Kupffer cells has not yet been
investigated in vivo. Here we present a novel approach,
which may be suitable for clinical application, to
selectively target NF-kB in Kupffer cells and analyse the
effects in experimental models of liver injury.
Methods: NF-kB inhibiting decoy oligodeoxynucleotides
were loaded upon gelatin nanoparticles (D-NPs) and their
in vivo distribution was determined by confocal microscopy.
Liver damage, NF-kB activity, cytokine levels and
apoptotic protein expression were evaluated after
lipopolysaccharide (LPS), D-galactosamine (GalN)/LPS, or
concanavalin A (ConA) challenge and partial warm
ischaemia and subsequent reperfusion, respectively.
Results: D-NPs were selectively taken up by Kupffer cells
and inhibited NF-kB activation. Inhibition of NF-kB in
Kupffer cells improved survival and reduced liver injury
after GalN/LPS as well as after ConA challenge. While
anti-apoptotic protein expression in liver tissue was not
reduced, pro-apoptotic players such as cJun N-terminal
kinase (JNK) were inhibited. In contrast, selective
inhibition of NF-kB augmented reperfusion injury.
Conclusions: NF-kB inhibiting decoy oligodeoxynucleotide-
loaded gelatin nanoparticles is a novel tool to
selectively inhibit NF-kB activation in Kupffer cells in vivo.
Thus, liver injury can be reduced in experimental fulminant
hepatitis, but increased at ischaemia–reperfusion
Multi-reference approach to the calculation of photoelectron spectra including spin-orbit coupling
X-ray photoelectron spectra provide a wealth of information on the electronic
structure. The extraction of molecular details requires adequate theoretical
methods, which in case of transition metal complexes has to account for effects
due to the multi-configurational and spin-mixed nature of the many-electron
wave function. Here, the Restricted Active Space Self-Consistent Field method
including spin-orbit coupling is used to cope with this challenge and to
calculate valence and core photoelectron spectra. The intensities are estimated
within the frameworks of the Dyson orbital formalism and the sudden
approximation. Thereby, we utilize an efficient computational algorithm that is
based on a biorthonormal basis transformation. The approach is applied to the
valence photoionization of the gas phase water molecule and to the core
ionization spectrum of the complex.
The results show good agreement with the experimental data obtained in this
work, whereas the sudden approximation demonstrates distinct deviations from
experiments
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