1,624 research outputs found
Impact of Black Shale Weathering on Sediment Quality
Weathering of black shales leads to elevated metal concentrations in both surface water and stream sediments. In spite of the recent focus on black shales, few data exist on the ecological impacts of this process particularly on aquatic organisms. The key objective of this study was to determine the impact of trace metal concentrations in sediments upon aquatic organisms. To achieve the above objective, stream sediment samples were collected from streams draining black shale and limestone (used as a reference stream) lithologies located in central Arkansas between June 2003 and January 2004. Trace metal concentrations were measured by the dynamic reaction cell inductively coupled plasma mass spectrometry (ICPMS; Perkin Elmer DRC II) following EPA 6020 methodology. Sediment samples were tested for toxicity using standard EPA protocols. The trace metal concentrations in sediments and acute toxicity test findings using midge larvae, Chironomus tentans with endpoints measured as growth and survival is presented. Our results showed that there are significant differences in survival of the midge larvae among the study sites and also among the different sampling occasions. Percent survival of the midge larvae in the sediments derived from black shales was lower than that observed in the limestone-derived stream sediments. Significant differences in growth of the midge larvae were also observed among the sites with the control and reference stream sediments having higher growth than the black shale stream sediments. Though our measured metal concentrations in the black shale-derived sediments were below the Effects Range-Low, there is a great potential of metal accumulation in the fine sediment fraction particularly during baseflow regimes. At the time, metals can be concentrated in the fine sediment fraction due to the low discharge and less dilution. The study thus far has shown that the black shale metal-enriched stream sediments have both lethal and sublethal effects on aquatic organisms and higher organisms through food chain transfer
Low-Cost Air Quality Monitoring Tools: From Research to Practice (A Workshop Summary).
In May 2017, a two-day workshop was held in Los Angeles (California, U.S.A.) to gather practitioners who work with low-cost sensors used to make air quality measurements. The community of practice included individuals from academia, industry, non-profit groups, community-based organizations, and regulatory agencies. The group gathered to share knowledge developed from a variety of pilot projects in hopes of advancing the collective knowledge about how best to use low-cost air quality sensors. Panel discussion topics included: (1) best practices for deployment and calibration of low-cost sensor systems, (2) data standardization efforts and database design, (3) advances in sensor calibration, data management, and data analysis and visualization, and (4) lessons learned from research/community partnerships to encourage purposeful use of sensors and create change/action. Panel discussions summarized knowledge advances and project successes while also highlighting the questions, unresolved issues, and technological limitations that still remain within the low-cost air quality sensor arena
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β-adrenergic-mediated dynamic augmentation of sarcolemmal CaV 1.2 clustering and co-operativity in ventricular myocytes.
Key pointsPrevailing dogma holds that activation of the β-adrenergic receptor/cAMP/protein kinase A signalling pathway leads to enhanced L-type CaV 1.2 channel activity, resulting in increased Ca2+ influx into ventricular myocytes and a positive inotropic response. However, the full mechanistic and molecular details underlying this phenomenon are incompletely understood. CaV 1.2 channel clusters decorate T-tubule sarcolemmas of ventricular myocytes. Within clusters, nanometer proximity between channels permits Ca2+ -dependent co-operative gating behaviour mediated by physical interactions between adjacent channel C-terminal tails. We report that stimulation of cardiomyocytes with isoproterenol, evokes dynamic, protein kinase A-dependent augmentation of CaV 1.2 channel abundance along cardiomyocyte T-tubules, resulting in the appearance of channel 'super-clusters', and enhanced channel co-operativity that amplifies Ca2+ influx. On the basis of these data, we suggest a new model in which a sub-sarcolemmal pool of pre-synthesized CaV 1.2 channels resides in cardiomyocytes and can be mobilized to the membrane in times of high haemodynamic or metabolic demand, to tune excitation-contraction coupling.AbstractVoltage-dependent L-type CaV 1.2 channels play an indispensable role in cardiac excitation-contraction coupling. Activation of the β-adrenergic receptor (βAR)/cAMP/protein kinase A (PKA) signalling pathway leads to enhanced CaV 1.2 activity, resulting in increased Ca2+ influx into ventricular myocytes and a positive inotropic response. CaV 1.2 channels exhibit a clustered distribution along the T-tubule sarcolemma of ventricular myocytes where nanometer proximity between channels permits Ca2+ -dependent co-operative gating behaviour mediated by dynamic, physical, allosteric interactions between adjacent channel C-terminal tails. This amplifies Ca2+ influx and augments myocyte Ca2+ transient and contraction amplitudes. We investigated whether βAR signalling could alter CaV 1.2 channel clustering to facilitate co-operative channel interactions and elevate Ca2+ influx in ventricular myocytes. Bimolecular fluorescence complementation experiments reveal that the βAR agonist, isoproterenol (ISO), promotes enhanced CaV 1.2-CaV 1.2 physical interactions. Super-resolution nanoscopy and dynamic channel tracking indicate that these interactions are expedited by enhanced spatial proximity between channels, resulting in the appearance of CaV 1.2 'super-clusters' along the z-lines of ISO-stimulated cardiomyocytes. The mechanism that leads to super-cluster formation involves rapid, dynamic augmentation of sarcolemmal CaV 1.2 channel abundance after ISO application. Optical and electrophysiological single channel recordings confirm that these newly inserted channels are functional and contribute to overt co-operative gating behaviour of CaV 1.2 channels in ISO stimulated myocytes. The results of the present study reveal a new facet of βAR-mediated regulation of CaV 1.2 channels in the heart and support the novel concept that a pre-synthesized pool of sub-sarcolemmal CaV 1.2 channel-containing vesicles/endosomes resides in cardiomyocytes and can be mobilized to the sarcolemma to tune excitation-contraction coupling to meet metabolic and/or haemodynamic demands
What do we know about the risks for young people moving into, through and out of inpatient mental health care? Findings from an evidence synthesis.
Background Young people with complex or severe mental health needs sometimes require care and treatment in inpatient settings. There are risks for young people in this care context, and this study addressed the question: ‘What is known about the identification, assessment and management of risk in young people (aged 11–18) with complex mental health needs entering, using and exiting inpatient child and adolescent mental health services in the UK?’ Methods In phase 1 a scoping search of two electronic databases (MEDLINE and PsychINFO) was undertaken. Items included were themed and presented to members of a stakeholder advisory group, who were asked to help prioritise the focus for phase 2. In phase 2, 17 electronic databases (EconLit; ASSIA; BNI; Cochrane Library; CINAHL; ERIC; EMBASE; HMIC; MEDLINE; PsycINFO; Scopus; Social Care Online; Social Services Abstracts; Sociological Abstracts; OpenGrey; TRiP; and Web of Science) were searched. Websites were explored and a call for evidence was circulated to locate items related to the risks to young people in mental health hospitals relating to ‘dislocation’ and ‘contagion’. All types of evidence including research, policies and service and practice responses relating to outcomes, views and experiences, costs and cost-effectiveness were considered. Materials identified were narratively synthesised. Results In phase 1, 4539 citations were found and 124 items included. Most were concerned with clinical risks. In phase 2, 15,662 citations were found, and 40 addressing the risks of ‘dislocation’ and ‘contagion’ were included supplemented by 20 policy and guidance documents. The quality of studies varied. Materials were synthesised using the categories: Dislocation: Normal Life; Dislocation: Identity; Dislocation: Friends; Dislocation: Stigma; Dislocation: Education; Dislocation: Families; and Contagion. No studies included an economic analysis. Although we found evidence of consideration of risk to young people in these areas we found little evidence to improve practice and services. Conclusions The importance to stakeholders of the risks of ‘dislocation’ and ‘contagion’ contrasted with the limited quantity and quality of evidence to inform policy, services and practice. The risks of dislocation and contagion are important, but new research is needed to inform how staff might identify, assess and manage them
Knowledge-Based Potential for Positioning Membrane-Associated Structures and Assessing Residue-Specific Energetic Contributions
The complex hydrophobic and hydrophilic milieus of membrane-associated proteins pose experimental and theoretical challenges to their understanding. Here we produce a non-redundant database to compute knowledge-based asymmetric cross-membrane potentials from the per-residue distributions of Cβ, Cγ and functional group atoms. We predict transmembrane and peripherally associated regions from genomic sequence and position peptides and protein structures relative to the bilayer (available at http://www.degradolab.org/ez). The pseudo-energy topological landscapes underscore positional stability and functional mechanisms demonstrated here for antimicrobial peptides, transmembrane proteins, and viral fusion proteins. Moreover, experimental effects of point mutations on the relative ratio changes of dual-topology proteins are quantitatively reproduced. The functional group potential and the membrane-exposed residues display the largest energetic changes enabling to detect native-like structures from decoys. Hence, focusing on the uniqueness of membrane-associated proteins and peptides, we quantitatively parameterize their cross-membrane propensity thus facilitating structural refinement, characterization, prediction and design
Recombination walking: genetic selection of clones from pooled libraries of yeast artificial chromosomes by homologous recombination.
A Model for the Analysis of Caries Occurrence in Primary Molar Tooth Surfaces
Recently methods of caries quantification in the primary dentition have moved away from summary ‘whole mouth’ measures at the individual level to methods based on generalised linear modelling (GLM) approaches or survival analysis approaches. However, GLM approaches based on logistic transformation fail to take into account the time-dependent process of tooth/surface survival to caries. There may also be practical difficulties associated with casting parametric survival-based approaches in a complex multilevel hierarchy and the selection of an optimal survival distribution, while non-parametric survival methods are not generally suitable for the assessment of supplementary information recorded on study participants. In the current investigation, a hybrid semi-parametric approach comprising elements of survival-based and GLM methodologies suitable for modelling of caries occurrence within fixed time periods is assessed, using an illustrative multilevel data set of caries occurrence in primary molars from a cohort study, with clustering of data assumed to occur at surface and tooth levels. Inferences of parameter significance were found to be consistent with previous parametric survival-based analyses of the same data set, with gender, socio-economic status, fluoridation status, tooth location, surface type and fluoridation status-surface type interaction significantly associated with caries occurrence. The appropriateness of the hierarchical structure facilitated by the hybrid approach was also confirmed. Hence the hybrid approach is proposed as a more appropriate alternative to primary caries modelling than non-parametric survival methods or other GLM-based models, and as a practical alternative to more rigorous survival-based methods unlikely to be fully accessible to most researchers
Tropospheric water vapor profiles obtained with FTIR: comparison with balloon-borne frost point hygrometers and influence on trace gas retrievals
Retrievals of vertical profiles of key atmospheric gases provide a critical
long-term record from ground-based Fourier transform infrared (FTIR) solar
absorption measurements. However, the characterization of the retrieved
vertical profile structure can be difficult to validate, especially for gases
with large vertical gradients and spatial–temporal variability such as water
vapor. In this work, we evaluate the accuracy of the most common water vapor
isotope (H216O, hereafter WV) FTIR retrievals in the lower and upper
troposphere–lower stratosphere. Coincident high-quality vertically resolved
WV profile measurements obtained from 2010 to 2016 with balloon-borne NOAA
frost point hygrometers (FPHs) are used as reference to evaluate the
performance of the retrieved profiles at two sites: Boulder (BLD), Colorado, and at
the mountaintop observatory of Mauna Loa (MLO), Hawaii. For a meaningful
comparison, the spatial–temporal variability has been investigated. We
present results of comparisons among FTIR retrievals with unsmoothed and
smoothed FPH profiles to assess WV vertical gradients. Additionally, we
evaluate the quantitative impact of different a priori profiles in the
retrieval of WV. An orthogonal linear regression analysis shows the best
correlation among tropospheric layers using ERA-Interim (ERA-I) a priori
profiles and biases are lower for unsmoothed comparisons. In Boulder, we
found a negative bias of 0.02±1.9 % (r = 0.95) for the 1.5–3 km
layer. A larger negative bias of 11.1±3.5 % (r = 0.97) was found in
the lower free troposphere layer of 3–5 km attributed to rapid vertical
change of WV, which is not always captured by the retrievals. The bias
improves in the 5–7.5 km layer (1.0±5.3 %, r = 0.94). The bias
remains at about 13 % for layers above 7.5 km but below 13.5 km. At MLO
the spatial mismatch is significantly larger due to the launch of the sonde
being farther from the FTIR location. Nevertheless, we estimate a negative
bias of 5.9±4.6 % (r = 0.93) for the 3.5–5.5 km layer and 9.9±3.7 % (r = 0.93) for the 5.5–7.5 km layer, and we measure positive biases of
6.2±3.6 % (r = 0.95) for the 7.5–10 km layer and 12.6 % and
greater values above 10 km. The agreement for the first layer is
significantly better at BLD because the air masses are similar for both FTIR
and FPH. Furthermore, for the first time we study the influence of different
WV a priori profiles in the retrieval of selected gas profiles. Using NDACC
standard retrievals we present results for hydrogen cyanide (HCN), carbon
monoxide (CO), and ethane (C2H6) by taking NOAA FPH profiles as the
ground truth and evaluating the impact of other WV profiles. We show that the
effect is minor for C2H6 (bias < 0.5 % for all WV sources) among all
vertical layers. However, for HCN we found significant biases between 6 %
for layers close to the surface and 2 % for the upper troposphere depending
on the
WV profile source. The best results (reduced bias and precision and r values
closer to unity) are always found for pre-retrieved WV. Therefore, we
recommend first retrieving WV to use in subsequent retrieval of gases.</p
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Steady state free radical budgets and ozone photochemistry during TOPSE
A steady state model, constrained by a number of measured quantities, was used to derive peroxy radical levels for the conditions of the Tropospheric Ozone Production about the Spring Equinox (TOPSE) campaign. The analysis is made using data collected aboard the NCAR/NSF C‐130 aircraft from February through May 2000 at latitudes from 40° to 85°N, and at altitudes from the surface to 7.6 km. HO2 + RO2 radical concentrations were measured during the experiment, which are compared with model results over the domain of the study showing good agreement on the average. Average measurement/model ratios are 1.04 (σ = 0.73) and 0.96 (σ = 0.52) for the MLB and HLB, respectively. Budgets of total peroxy radical levels as well as of individual free radical members were constructed, which reveal interesting differences compared to studies at lower latitudes. The midlatitude part of the study region is a significant net source of ozone, while the high latitudes constitute a small net sink leading to the hypothesis that transport from the middle latitudes can explain the observed increase in ozone in the high latitudes. Radical reservoir species concentrations are modeled and compared with the observations. For most conditions, the model does a good job of reproducing the formaldehyde observations, but the peroxide observations are significantly less than steady state for this study. Photostationary state (PSS) derived total peroxy radical levels and NO/NO2 ratios are compared with the measurements and the model; PSS‐derived results are higher than observations or the steady state model at low NO concentrations
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