A WIDE DISTRIBUTION OF A NEW VRN-B1c ALLELE OF WHEAT TRITICUM AESTIVUM L. IN RUSSIA, UKRAINE AND ADJACENT REGIONS: A LINK WITH THE HEADING TIME AND ADAPTIVE POTENTIAL

The adaptation of common wheat (T. aestivum L.) to diverse environmental conditions is greatly under the control of genes involved in determination of vernalization response (Vrn-1 genes). It was found that the variation in common wheat heading time is affected not only by combination of Vrn-1 homoeoalleles but also by multiple alleles at a separate Vrn-1 locus. Previously, we described the Vrn-B1c allele from T.aestivum cv. 'Saratovskaya 29' and found significant differences in the structure of the first (1st) intron of this allele when compared to another highly abundant Vrn-B1a allele, specifically, the deletion of 0.8 kb coupled with the duplication of 0.4 kb. We suggested that the changes in the intron 1 of Vrn-B1c allele caused earlier ear emergence in the near-isogenic line and cultivars, carrying this allele. In this study we investigate the distribution of the Vrn-B1c allele in a wide set of spring wheat cultivars from Russia, Ukraine and adjacent regions. The analysis revealed that 40% of Russian and 53% of Ukranian spring wheat cultivars contain the Vrn-B1c allele. The high distribution of the Vrn-B1c allele can be explained by a frequent using of 'Saratovskaya 29' in the breeding process inside the studied area. From the other hand, the predominance of the Vrn-B1c allele among cultivars cultivated in West Siberia and Kazakhstan may be due to the selective advantage of this allele for the region where there is a high risk of early fall frosts

DELLA MUTATIONS IN PLANTS WITH SPECIAL EMPHASIS ON WHEAT

DELLA proteins are a subfamily of transcriptional regulators from the GRAS protein family. They act as growth repressors. Gibberellins (GA) promote growth by overcoming the DELLA-mediated growth restraint. A number of dominant mutations in highly conserved orthologous DELLA genes are known in various plant species. These mutations reduce plant height owing to production of more active forms of growth repressors. The aim of the current study is to estimate the genetic similarity of DELLA nucleotide and amino acid sequences stored in GenBank and to identify mutant and wild type alleles that encode DELLAs in modern Ukrainian winter bread wheat varieties. We have performed nucleotide and amino acid sequence search and alignment of the Rht-D1 wheat dwarfing gene and DELLA-encoding genes of other plant species with BLAST and MUSCLE software. A dendrogram illustrating phylogenetic relations among DELLA sequences of 35 plant species has been constructed using MEGA v5. In addition, PCR-based genotyping for the major wheat dwarfing genes Rht-B1 and Rht-D1 has been performed in wheat cultivars from different regions of Ukraine. It is shown that the mutant Rht-D1b allele is the commonest dwarfing allele among those tested in Ukrainian wheat cultivars (68 %), but its distribution over Ukraine is not random

Prevention of sudden death in children during physical training classes

The article presents the methods of prevention of sudden cardiac death in children and adolescents, including questioning of parents in order to identify children with an increased risk of sudden cardiac death in physical training classes.В статье отражены методы профилактики внезапной сердечной смерти у детей и подростков. Уделяется особое внимание анкетированию родителей с целью выявления детей с повышенным риском наступления внезапной сердечной смерти

Evaluation of the potential accuracy of the pulsed radio signals source coordinate determination by the positioning system using a single UAV-sensor

An approach to determining the coordinates of pulsed radio signals sources by the positioningsystem in the application of a single unmanned aerial vehicles sensor is shown in this article. Therange-difference location method allows to determinate an informative coordinate parameter withoutretransmission and internal synchronization of receiving points. The result is achieved by improving thewell-known scientific and methodological apparatus with considering some features of the operationmode of pulsed radio signals sources. Presenting results of the simulation allow to estimate theinfluence of main input factors on the potential accuracy of the pulsed radio signal source coordinatedetermination

Состояние колонизационной резистентности у хирургических больных в раннем послеоперационном периоде

We have examined one of the main components of the mechanism of purulent-inflammation complications in surgical patients. It is normal microflora which reflects the condition of colonization resistance of the organism which is a leading mechanism of opposition to hospital infections. We have established changes of microflora of different biotopes of the organism in dependence on pathologic focus localization and of the period after the surgery. Complex approach to the investigation of colonization resistance allowed to discover not only dysbiotic changes but disturbances of some immunological mechanisms of total and local anti- infectious protection in surgical patients. It is desirable to combine selective decontamination with taking drugs which normalize indigenous micro-flora of the host (special diets, growth stimulators of normal microflora representatives, eubiotics) and immune-stimulating therapy.Исследован один из главных компонентов пускового механизма гнойно-воспалительных осложнений у хирургических больных - нормальная микрофлора, отражающая состояние колонизационной резистентности организма - ведущего защитного механизма противодействия госпитальным инфекциям. Установлены изменения микрофлоры различных биотопов организма больных в зависимости от локализации патологического очага и срока после операции. Комплексный подход к изучению колонизационной резистентности позволил выявить не только дисбиотические изменения, но и нарушения некоторых иммунных механизмов общей и местной противоинфекционной защиты у хирургических больных. Селективную деконтаминацию желательно сочетать с приемом препаратов, нормализующих индигенную микрофлору хозяина (специальные диеты, стимуляторы роста представителей нормальной флоры, назначение эубиотиков), и иммуностимулирующей терапией

СЛУЧАЙ УСПЕШНОГО ПРИМЕНЕНИЯ ЧРЕСКОЖНОЙ ВРЕМЕННОЙ ОККЛЮЗИИ ВЕНОЗНОГО СИНУСА СЕРДЦА У БОЛЬНОГО С ОСТРЫМ КОРОНАРНЫМ СИНДРОМОМ С ЭЛЕВАЦИЕЙ СЕГМЕНТА ST ПРИ ОТСУТСТВИИ ГЕМОДИНАМИЧЕСКИ ЗНАЧИМОГО АТЕРОСКЛЕРОТИЧЕСКОГО ПОРАЖЕНИЯ КОРОНАРНЫХ АРТЕРИЙ

The search for new methods of adequate antegrade blood flow restoration in the coronary arteries of patients with acute coronary syndrome with ST-segment elevation (ACSesST) without hemodynamically significant atherosclerotic lesions of the coronary arteries is a poorly studied item. The author gives the clinical example of a patient with ACSesST without hemodynamically significant atherosclerotic lesions of the coronary arteries and deceleration of antegrade blood flow in the area of the anterior descending artery (ADA). Percutaneous temporary occlusion of the coronary sinus was done to the patient during 10 minutes in the balloon catheter «inflation-deflation». The procedure resulted in a positive impact on coronary hemodynamics such as distal coronary spasm cupping, myocardial perfusion improvement and restoration of adequate antegrade blood flow in the pool ADA.Поиск новых способов восстановления адекватного антеградного кровотока по коронарным артериям у пациентов с острым коронарным синдромом с подъемом сегмента ST (ОКСспST) без гемодинамически значимого атеросклеротического поражения коронарного русла является малоизученной проблемой. Приведен клинический пример больного с ОКСспST без гемодинамически значимого поражения коронарных артерий и замедлением антеградного кровотока по бассейну передней нисходящей артерии (ПНА). Пациенту была проведена чрескожная временная окклюзия коронарного синуса в течение 10 минут в режиме «инфляции-дефляции» баллонного катетера. Процедура оказала непосредственное положительное воздействие на коронарную гемодинамику в виде купирования дистального коронароспазма, улучшения миокардиальной перфузии и восстановления адекватного антеградного кровотока по бассейну ПНА