Role of Cellular Heparan Sulfate Proteoglycans in Infection of Human Adenovirus Serotype 3 and 35

Species B human adenoviruses (Ads) are increasingly associated with outbreaks of acute respiratory disease in U.S. military personnel and civil population. The initial interaction of Ads with cellular attachment receptors on host cells is via Ad fiber knob protein. Our previous studies showed that one species B Ad receptor is the complement receptor CD46 that is used by serotypes 11, 16, 21, 35, and 50 but not by serotypes 3, 7, and 14. In this study, we attempted to identify yet-unknown species B cellular receptors. For this purpose we used recombinant Ad3 and Ad35 fiber knobs in high-throughput receptor screening methods including mass spectrometry analysis and glycan arrays. Surprisingly, we found that the main interacting surface molecules of Ad3 fiber knob are cellular heparan sulfate proteoglycans (HSPGs). We subsequently found that HSPGs acted as low-affinity co-receptors for Ad3 but did not represent the main receptor of this serotype. Our study also revealed a new CD46-independent infection pathway of Ad35. This Ad35 infection mechanism is mediated by cellular HSPGs. The interaction of Ad35 with HSPGs is not via fiber knob, whereas Ad3 interacts with HSPGs via fiber knob. Both Ad3 and Ad35 interacted specifically with the sulfated regions within HSPGs that have also been implicated in binding physiologic ligands. In conclusion, our findings show that Ad3 and Ad35 directly utilize HSPGs as co-receptors for infection. Our data suggest that adenoviruses evolved to simulate the presence of physiologic HSPG ligands in order to increase infection

Species Interactions during Diversification and Community Assembly in an Island Radiation of Shrews

Closely related, ecologically similar species often have adjacent distributions, suggesting competitive exclusion may contribute to the structure of some natural communities. In systems such as island archipelagos, where speciation is often tightly associated with dispersal over oceanic barriers, competitive exclusion may prevent population establishment following inter-island dispersal and subsequent cladogenesis.) species in the Philippines are the result of competitive exclusion preventing secondary invasion of occupied islands. We first compare ecological niche models between two widespread, allopatric species and find statistical support for their ecological similarity, implying that competition for habitat between these species is possible. We then examine dispersion patterns among sympatric species and find some signal for overdispersion of body size, but not for phylogenetic branch length. Finally, we simulate the process of inter-island colonization under a stochastic model of dispersal lacking ecological forces. Results are dependent on the geographic scope and colonization probability employed. However, some combinations suggest that the number of inter-island dispersal events necessary to populate the archipelago may be much higher than the minimum number of colonization events necessary to explain current estimates of species richness and phylogenetic relationships. If our model is appropriate, these results imply that alternative factors, such as competitive exclusion, may have influenced the process of inter-island colonization and subsequent cladogenesis.We interpret the combined results as providing tenuous evidence that similarity in body size may prevent co-occurrence in Philippine shrews and that competitive exclusion among ecologically similar species, rather than an inability to disperse among islands, may have limited diversification in this group, and, possibly other clades endemic to island archipelagos

New features from the Romblon Island Group: Key to understanding the arc–continent collision in Central Philippines

International audienceA complete ophiolite sequence and mélange are reported for the first time in the Romblon Island Group located at the central part of the Philippine archipelago. The study area comprises Tablas, Romblon and Sibuyan Islands built on amalgamated crust and mantle rocks that are typically fault-bounded and stratigraphically disrupted. The Sibuyan Ophiolite Complex (SOC) consists of harzburgite and dunite, layered clinopyroxenite, layered and isotropic gabbro, diabase dike swarm and basaltic–andesitic pillow lava and flow deposits. A Jurassic–Cretaceous age of formation and late Early Miocene age of emplacement are suggested for the SOC by available data. Emplacement of these crust–mantle units is believed to have been facilitated through displacements along east-verging thrust faults developed consequent to the Early Miocene arc–continent collision in Central Philippines. Along with recent K–Ar isotopic ages (12 Ma) obtained for some of the metamorphic rocks in the area, these lithologic units and structural features provide critical information regarding the arc–continent collision which is one of the significant events in the evolution of the Philippine island arc system. The usefulness of potential field geophysical methods to studying lithologic and structural relationships in an extremely disrupted terrane is evaluated in this study. Observed variations in gravity and magnetic values correlated with lithologic distributions and geologic structures. Higher relative Bouguer and magnetic anomalies are observed over members of the complete ophiolite sequence and younger igneous materials exposed in Tablas and Sibuyan Islands. Their geophysical characteristics contrast markedly with the less dense and magnetically inferior sedimentary rocks exposed only on Tablas Island. On the other hand, metamorphic units in Tablas, Romblon and Sibuyan Islands display variable magnetic and gravity signatures that reflect differences in composition and alteration or weathering. Thrust faults related to the emplacement of the ophiolite as a result of the collision are inferred from sudden changes in gravity and magnetic value

Boninite and boninite-series volcanics in northern Zambales ophiolite: doubly vergent subduction initiation along Philippine Sea plate margins

A key component of subduction initiation rock suites is boninite, a high-magnesium andesite that is uniquely predominant in western Pacific forearc terranes and in select Tethyan ophiolites such as Oman and Troodos. We report, for the first time, the discovery of low-calcium, high-silica boninite in the middle Eocene Zambales ophiolite (Luzon Island, Philippines). Olivine–orthopyroxene microphyric high-silica boninite, olivine–clinopyroxene–phyric low-silica boninite and boninitic basalt occur as lapilli fall deposits and pillow lava flows in the upper volcanic unit of the juvenile arc section (Barlo locality, Acoje Block) of the Zambales ophiolite. This upper volcanic unit overlies a lower volcanic unit consisting of basaltic andesite, andesite to dacitic lavas and explosive eruptive material (subaqueous pahoehoe and lobate sheet flows, agglutinate and spatter deposits) forming a low-silica boninite series. The overall volcanic stratigraphy of the extrusive sequence at Barlo resembles holes U1439 and U1442 drilled by IODP Expedition 352 in the Izu–Ogasawara (Bonin) trench slope. The presence of depleted proto-arc basalts in the Coto Block (45 Ma) (Geary et al., 1989), boninite and boninite series volcanics in Barlo (Acoje Block (44 Ma)) and simultaneous and post-boninite moderate-Fe arc tholeiites in Sual and Subic areas of the Acoje Block (44–43 Ma) indicate that the observed subduction initiation stratigraphy in the Izu–Ogasawara–Mariana forearc is also present in the Zambales ophiolite. Paleolatitudes derived from tilt-corrected sites in the Acoje Block place the juvenile arc of northern Zambales ophiolite in the western margin of the Philippine Sea plate. In this scenario, the origin of Philippine Sea plate boninites (IBM and Zambales) would be in a doubly vergent subduction initiation setting