Increased susceptibility to Trichuris muris infection and exacerbation of colitis in Mdr1a-/- mice

AIM: To investigate the influence of Trichuris muris (T. muris ) infection in a mouse model of genetic susceptibility to inflammatory bowel disease, Mdr1a-/-. METHODS: Mdr1a-/- mice were housed under specific pathogen free conditions to slow the development of colitis and compared to congenic FVB controls. Mice were infected with approximately 200 embryonated ova from T. muris and assessed for worm burden and histological and functional markers of gut inflammation on day 19 post infection. RESULTS: Mdr1a-/- mice exhibited a marked increase in susceptibility to T. muris infection with a 10-fold increase in colonic worm count by day 19 pi compared to FVB controls. Prior to infection, Mdr1a-/- exhibited low-level mucosal inflammation with evidence of an enhanced Th1 environment. T. muris infection accelerated the progression of colitis in Mdr1a-/- as evidenced by marked increases in several indicators including histological damage score, mucosal CD4+ T-cell and DC infiltration and dramatically increased production of proinflammatory cytokines. CONCLUSION: These data provide further evidence of the complex interaction between T. muris and an inflammatory bowel disease (IBD)-susceptible host which may have relevance to the application of helminth therapy in the treatment of human IBD

Labour supply and skills demands in fashion retailing

If, as Adam Smith once famously suggested, Britain was a nation of shopkeepers then it is now a nation of shopworkers. Retail is now a significant part of the UK economy, accounting for £256 billion in sales and one-third of all consumer spending (Skillsmart, 2007). It is the largest private sector employer in the UK, employing 3m workers, or 1 in 10 of the working population. For future job creation in the UK economy retail is also similarly prominent and the sector is expected to create a further 250,000 jobs to 2014 (Skillsmart, 2007). The centrality of retail to economic success and job creation is apparent in other advanced economies. For example, within the US, retail sales is the occupation with the largest projected job growth in the period 2004-2014 (Gatta et al., 2009) and in Australia retail accounts for 1 in 6 workers (Buchanan et al., 2003). Within the UK these workers are employed in approximately 290,000 businesses, encompassing large and small organizations and also a number of sub-sectors. This variance suggests that retail should not be regarded as homogenous in its labour demands. Hart et al. (2007) note how skill requirements and the types of workers employed may differ across the sector. This chapter further opens up this point, providing an analysis of the labour supply and skills demands for the sub-sectors of clothing, footwear and leather goods, which are described by Skillsmart (2007: 48) as being 'significant categories in UK retailing'

Activated carbon from Moringa husks and pods

Moringa oleifera is the most widespread species of the plant family Moringaceae. It is a rapidly growing tree native to the sub-Himalayan regions of north-west India and indigenous to many parts of Africa, South America and Asia, typically reaching a height of 3-4 metres, flowering and fruiting in one year from a 0.3 metre seedling even in poor quality soil (Sutherland, Folkard et al., 1994). It is known by different names around the world (Jahn, 1986), for example in India “Drumstick” or “Horseradish” tree. The tree produces large seed pods which can either be harvested when green for food or left to dry. The dried seeds can be crushed to produce a high quality vegetable oil and the resulting press-cake mixed with water and strained to form a coagulant for water treatment (McConnachie, Mtawali et al., 1994). The residue containing seed husks is currently discarded as waste. The research presented here has been carried out at the University of Edinburgh and the University of Malawi and demonstrates that a simple steam pyrolysis procedure can form high quality microporous activated carbons from both the waste husks of Moringa oleifera and the pods. The work is part of an overall assessment of the viability of the establishment of Moringa oleifera plantations in tropical areas for the benefit of rural communities and is being carried out in collaboration with the Engineering Department, The University of Leicester, U.K

Odour testing using moringa carbon

Provision of safe and potable drinking water to urban communities is a challenge to professionals in many low and middle income countries (WEDC, 1996). The situation is aggravated in those under-developed countries where agriculture is the mainstay of the economy. Fertilisers are extensively used in these countries and this leads to increased run-off of nutrients into surface water sources, with the negative consequence of prolific algal growths in the receiving waters. In Malawi, surface waters are the main source of drinking water, with only a small percentage of the supply being drawn from groundwater supplies. These surface waters are invariably infested with algae. This paper assesses the degree of odour removal at a conventional water treatment plant (Lilongwe Water Works, Malawi) and a direct filtration plant (Blantyre water works, Malawi). The effect of ageing in a rapid gravity sand filter was also investigated to assess its effectiveness in odour removal as the filter-run progressed

What we know and what we need to know about graduate skills

The expansion of higher education has led to more graduates in the UK labour market. Despite government expectations, this expansion has not boosted national economic competitiveness. This article argues that current understanding of the impact of graduates’ skills is limited by methodological and conceptual narrowness in current research and that a broader research agenda is required. This agenda needs to cover not just the supply but also the demand, development and deployment of graduates’ skills and, as a consequence, distinguish between ‘graduate skills’ acquired in higher education and the ‘skills of graduates’ formed prior to, in and parallel to higher education

Giving Miss Marple a makeover : graduate recruitment, systems failure and the Scottish voluntary sector

The voluntary sector in Scotland, as across the globe, is becoming increasingly business like. Resultantly, there is an increasing demand for graduates to work in business and support functions. In Scotland, however, despite an oversupply of graduates in the labor market, the voluntary sector reports skills shortages for graduate-level positions; a leadership deficit was also reported in countries such as the United States. Through exploratory, mainly qualitative, case study and stakeholder research, this article proposes that one reason for this mismatch between the supply of and demand for graduates is a systems failure within the sector. Many graduates and university students remain unaware of potentially suitable paid job opportunities, in part because of the sector's voluntary label. To rectify this systems failure, thought needs to be given to the sector's nomenclature and the manner in which voluntary sector organizations attract graduate recruits, for example, through levering value congruence in potential recruits

Validation of a chloroquine-induced cell death mechanism for clinical use against malaria

An alternative antimalarial pathway of an ‘outdated’ drug, chloroquine (CQ), may facilitate its return to the shrinking list of effective antimalarials. Conventionally, CQ is believed to interfere with hemozoin formation at nanomolar concentrations, but resistant parasites are able to efflux this drug from the digestive vacuole (DV). However, we show that the DV membrane of both resistant and sensitive laboratory and field parasites is compromised after exposure to micromolar concentrations of CQ, leading to an extrusion of DV proteases. Furthermore, only a short period of exposure is required to compromise the viability of late-stage parasites. To study the feasibility of this strategy, mice malaria models were used to demonstrate that high doses of CQ also triggered DV permeabilization in vivo and reduced reinvasion efficiency. We suggest that a time-release oral formulation of CQ may sustain elevated blood CQ levels sufficiently to clear even CQ-resistant parasites

Older workers in the hospitality industry: valuing experience and informal learning

The research sets out to identify the learning processes adopted by older workers in the hospitality and visitor attraction industry in Scotland, with a view to determining how employers may better support their education and training within enterprises. The study was undertaken as part of the ESRC project on ‘sustaining the employability of older workers in the hospitality sector: personal learning strategies and cultures of learning. The data collection period was from 2008-2010 and focused on six case studies; three in hospitality and three visitor attraction centres. The conceptual framework of the research is based upon the simple yet important notion of experience and how this enhances the learning lives of older employees. It will be argued that the learning processes used by older employees are primarily recognisable as social practices, based upon the utilisation of existing knowledge and skills. The analysis suggests that organisations should be encouraged to avoid using a ‘one size fits all’ approach to education and training and, in the case of older workers, to make more use of their past work and life experiences in order to facilitate their own and others’ learning