DEVELOPMENT AND VALIDATION OF HPLC METHOD FOR SIMULTANEOUS QUANTIFICATION OF VASICINE, GLYCYRRHIZIN AND PIPERINE IN POLY HERBAL COUGH SYRUP

Objective: The present study was undertaken to develop a rapid, simple, specific and economic high performance liquid chromatographic (HPLC) method has been developed, validated and used for simultaneous quantification of Vasicine, Glycyrrhizin and Piperine in poly herbal cough syrup Methods: An Agilent technologies 1200 Series quaternary pump combined with an Agilent 1200 series photo diode array detector (USA), an Agilent 1200 series vacuum degasser (USA) and an Agilent autosampler injector. Chromatographic separation was performed on a Hiber, prepacked column, C18, Size 250x 4.60 mm, 5µ maintained at 25 °C. PJ (solvent A) and HPLC grade Acetonitrile (solvent B). Results: The HPLC developed for quantization was simple, accurate and specific. The drug follows the beer’s lambert’s law in the concentration range of of Vasicine in concentration range 25–250 μg/ml, glycyrrhizin in concentration range 100–1000 μg/ml and Piperine in concentration range of 20–100μg/ml and exhibited good correlation coefficient and excellent mean recovery. Percentage RSD for precision and accuracy of the method was found to be less than 2%. Conclusion: The present standardization provides a specific and rapid tool in the herbal research, permitting to set quality specifications for identity, transparency and reproducibility of these Phytoconsituents in the herbal Cough syrup

Life in times of COVID-19

Should vegetables be washed with soap before using them? Can COVID-19 spread from newspapers, coins, bank notes, ATM cards, or air conditioning? Do Indians have innate immunity against SARS�CoV-2? How do we use herd immunity to our advantage

Pacemakers and Implantable Cardioverter Defibrillator

The history of cardiac pacing therapy must be viewed within the broader framework of electro-diagnosis and electro-therapy. Electro-therapy has a simple core concept: the use of an outside source of electricity to stimulate human tissue in various ways to produce a beneficial therapeutic effect. This has shown a prolonged, halting development through the ages, sometimes being looked upon as mysterious magic produced by complex machines. The field of paediatric open heart surgery gave a major impetus to the development of pacemakers since heart block often accompanied impeccably performed intra-cardiac repairs of congenital defects. This review deals various of aspects of pacemaker functioning, indications, contraindications and complications

Genera of phytopathogenic fungi: GOPHY 3

This paper represents the third contribution in the Genera of Phytopathogenic Fungi (GOPHY) series. The series provides morphological descriptions, information about the pathology, distribution, hosts and disease symptoms for the treated genera, as well as primary and secondary DNA barcodes for the currently accepted species included in these. This third paper in the GOPHY series treats 21 genera of phytopathogenic fungi and their relatives including: Allophoma, Alternaria, Brunneosphaerella, Elsinoe, Exserohilum, Neosetophoma, Neostagonospora, Nothophoma, Parastagonospora, Phaeosphaeriopsis, Pleiocarpon, Pyrenophora, Ramichloridium, Seifertia, Seiridium, Septoriella, Setophoma, Stagonosporopsis, Stemphylium, Tubakia and Zasmidium. This study includes three new genera, 42 new species, 23 new combinations, four new names, and three typifications of older names