Simulation and experimental verification of W-band finite frequency selective surfaces on infinite background with 3D full wave solver NSPWMLFMA

We present the design, processing and testing of a W-band finite by infinite and a finite by finite Grounded Frequency Selective Surfaces (FSSs) on infinite background. The 3D full wave solver Nondirective Stable Plane Wave Multilevel Fast Multipole Algorithm (NSPWMLFMA) is used to simulate the FSSs. As NSPWMLFMA solver improves the complexity matrix-vector product in an iterative solver from O(N(2)) to O(N log N) which enables the solver to simulate finite arrays with faster execution time and manageable memory requirements. The simulation results were verified by comparing them with the experimental results. The comparisons demonstrate the accuracy of the NSPWMLFMA solver. We fabricated the corresponding FSS arrays on quartz substrate with photolithographic etching techniques and characterized the vector S-parameters with a free space Millimeter Wave Vector Network Analyzer (MVNA)

Probing Differences in Gene Essentiality Between the Human and Animal Adapted Lineages of the Mycobacterium tuberculosis Complex Using TnSeq.

Members of the Mycobacterium tuberculosis complex (MTBC) show distinct host adaptations, preferences and phenotypes despite being >99% identical at the nucleic acid level. Previous studies have explored gene expression changes between the members, however few studies have probed differences in gene essentiality. To better understand the functional impacts of the nucleic acid differences between Mycobacterium bovis and Mycobacterium tuberculosis, we used the Mycomar T7 phagemid delivery system to generate whole genome transposon libraries in laboratory strains of both species and compared the essentiality status of genes during growth under identical in vitro conditions. Libraries contained insertions in 54% of possible TA sites in M. bovis and 40% of those present in M. tuberculosis, achieving similar saturation levels to those previously reported for the MTBC. The distributions of essentiality across the functional categories were similar in both species. 527 genes were found to be essential in M. bovis whereas 477 genes were essential in M. tuberculosis and 370 essential genes were common in both species. CRISPRi was successfully utilised in both species to determine the impacts of silencing genes including wag31, a gene involved in peptidoglycan synthesis and Rv2182c/Mb2204c, a gene involved in glycerophospholipid metabolism. We observed species specific differences in the response to gene silencing, with the inhibition of expression of Mb2204c in M. bovis showing significantly less growth impact than silencing its orthologue (Rv2182c) in M. tuberculosis. Given that glycerophospholipid metabolism is a validated pathway for antimicrobials, our observations suggest that target vulnerability in the animal adapted lineages cannot be assumed to be the same as the human counterpart. This is of relevance for zoonotic tuberculosis as it implies that the development of antimicrobials targeting the human adapted lineage might not necessarily be effective against the animal adapted lineage. The generation of a transposon library and the first reported utilisation of CRISPRi in M. bovis will enable the use of these tools to further probe the genetic basis of survival under disease relevant conditions

Exploring the symbiotic pangenome of the nitrogen-fixing bacterium Sinorhizobium meliloti

<p>Abstract</p> <p>Background</p> <p><it>Sinorhizobium meliloti </it>is a model system for the studies of symbiotic nitrogen fixation. An extensive polymorphism at the genetic and phenotypic level is present in natural populations of this species, especially in relation with symbiotic promotion of plant growth. AK83 and BL225C are two nodule-isolated strains with diverse symbiotic phenotypes; BL225C is more efficient in promoting growth of the <it>Medicago sativa </it>plants than strain AK83. In order to investigate the genetic determinants of the phenotypic diversification of <it>S. meliloti </it>strains AK83 and BL225C, we sequenced the complete genomes for these two strains.</p> <p>Results</p> <p>With sizes of 7.14 Mbp and 6.97 Mbp, respectively, the genomes of AK83 and BL225C are larger than the laboratory strain Rm1021. The core genome of Rm1021, AK83, BL225C strains included 5124 orthologous groups, while the accessory genome was composed by 2700 orthologous groups. While Rm1021 and BL225C have only three replicons (Chromosome, pSymA and pSymB), AK83 has also two plasmids, 260 and 70 Kbp long. We found 65 interesting orthologous groups of genes that were present only in the accessory genome, consequently responsible for phenotypic diversity and putatively involved in plant-bacterium interaction. Notably, the symbiosis inefficient AK83 lacked several genes required for microaerophilic growth inside nodules, while several genes for accessory functions related to competition, plant invasion and bacteroid tropism were identified only in AK83 and BL225C strains. Presence and extent of polymorphism in regulons of transcription factors involved in symbiotic interaction were also analyzed. Our results indicate that regulons are flexible, with a large number of accessory genes, suggesting that regulons polymorphism could also be a key determinant in the variability of symbiotic performances among the analyzed strains.</p> <p>Conclusions</p> <p>In conclusions, the extended comparative genomics approach revealed a variable subset of genes and regulons that may contribute to the symbiotic diversity.</p

Geographische Prognostik aus der Sicht bestehender und möglicher Anwendungsfelder : neue Anforderungen an eine Disziplin mit Tradition

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A multi-valley model for hot free-electron nonlinearities at 10.6

When the frequency of infrared light and the plasma frequency of highly doped n-GaAs are in resonance (e.g. for a doping concentration N = 7 × 1018cm−3 and a wavelength $\lambda=10.6 \mu$m), the free-electron induced optical nonlinearity is soundly pronounced. At such high doping concentrations it is necessary to extend the rigid quantum mechanical description of the free-electron induced nonlinearity to a multi-valley model. The central valley of GaAs was treated as a fully nonparabolic degenerated electron gas, whereas the satellite valley was modeled as an anisotropic electron gas of arbitrary degeneracy. The following intra- and intervalley absorption mechanisms were taken into account: impurity assisted, thermal and hot polar optical phonon assisted intravalley absorption on one hand and intervalley phonon assisted absorption in equivalent and nonequivalent intervalley absorption on the other hand. The dependence of the different absorption and energy relaxation mechanisms on the doping concentration, free electron heating, optical power density and the equivalent LL-intervalley deformation potential are discussed. We demonstrated for the first time that the behavior of the optical intervalley nonlinearity, i.e. the nonlinear absorption and nonlinear intervalley transfer, strongly depend on the equivalent LL-intervalley deformation potential. In the linear regime the model calculations are in good agreement with experimental results

X-valley influence on hot free electron absorption and optical nonlinearities at 10.6

A theoretical overview is given about the influence of the presence of the X-valley in highly doped n-GaAs on hot free-electron absorption and optical nonlinearities at 10.6 μm wavelength. The implications of the extension of the quantum-mechanical model from two to three valleys are discussed. For electron temperatures above 600 K the X-valley presence starts to be observed. We reveal that it is difficult to trace the individual contributions of different X-electron related inter- and intravalley absorption and relaxation phenomena and therefore we suggest to introduce an effective X-valley related deformation potential which is a weighted combination of all the X-valley contributions. We discuss how nonlinear optical experiments can be conducted to determine the LL-intervalley and this effective X-valley deformation potential