Employing the model based systems engineering methodologies to develop a domain specific language for contracting of infrastructure projects

© 2018 IEEE. The procurement of infrastructure systems by the public sector is very costly, long and not transparent since the processes are based on preparing huge amounts of documents which are difficult to be kept consistent and to be used (e.g. bid evaluation). Acquisition architecture frameworks (AF) are metamodels, developed to model the whole enterprise/system life cycle stages including system procurement. Our previous study analyzed the currently used AFs such as DoDAF, MoDAF and TRAK to assess their adequacy and efficiency in modelling the infrastructure projects. The results showed that many of the procurement related concerns are overlooked such as financial matters e.g. cost and revenue calculation; and risk management aspects e.g. risk calculation and risk allocation. This paper focuses on identifying the procurement concerns and adding new viewpoints to the architecture frameworks; and developing a domain specific language based on SysML to model the new viewpoints. A methodology is provided which shows how the metamodel (abstract syntax) and the language stereotypes (concrete syntax) are developed. The results firstly show the 18 identified viewpoints of procurement domain and then one of them (project funding) is chosen to be described in this paper. The conceptual definition of the 'project funding' viewpoint and the models it generates are illustrated as example diagrams of this DSL. This DSL can be used by the domain practitioners, who are the contracting officers and procurement managers, to generate the contracting materials to facilitate the contracting process, assure the consistency of the procurement documents, giving better project outcomes

Application of design science research to design a modelling approach for procurement of infrastructure systems

© 2019 IEEE. Model-driven approaches are widely used in managing the complex domains such as infrastructure systems or disaster management. The foundation of conducting a systematic research is designing a methodology that pertinently covers the steps of research from problem definition to solution proposal and then identifying or tailoring a method for developing and validating the solution. This paper explains the application of Design Science for conducting a research which aims at providing a model-driven approach for addressing the complexities of infrastructure procurement projects. So firstly the design science artefacts are adopted for designing the method for this research. Then the steps of this method are explained briefly along with description of how each step is applied in this research. The core of this method is proposing a process for developing and validating the metamodels which is designed based on combination of other metamodeling processes

Flood–pedestrian simulator for modelling human response dynamics during flood-induced evacuation : Hillsborough stadium case study

The flood–pedestrian simulator uses a parallel approach to couple a hydrodynamic model to a pedestrian model in a single agent-based modelling (ABM) framework on graphics processing units (GPU), allowing dynamic exchange and processing of multiple-agent information across the two models. The simulator is enhanced with more realistic human body characteristics and in-model behavioural rules. The new features are implemented in the pedestrian model to factor in age- and gender-related walking speeds for the pedestrians in dry zones around the floodwater and to include a maximum excitement condition. It is also adapted to use age-related moving speeds for pedestrians inside the floodwater, with either a walking condition or a running condition. The walking and running conditions are applicable without and with an existing two-way interaction condition that considers the effects of pedestrian congestion on the floodwater spreading. A new autonomous change of direction condition is proposed to make pedestrian agents autonomous in wayfinding decisions driven by their individual perceptions of the flood risk or the dominant choice made by the others. The relevance of the newly added characteristics and rules is demonstrated by applying the augmented simulator to reproduce a synthetic test case of a flood evacuation in a shopping centre, to then contrast its outcomes against the version of the simulator that does not consider age and gender in the agent characteristics. The enhanced simulator is demonstrated for a real-world case study of a mass evacuation from the Hillsborough football stadium, showing usefulness for flood emergency evacuation planning in outdoor spaces where destination choice and individual risk perception have great influence on the simulation outcomes

Heavy metal accumulation in Artemisia and foliaceous lichen species from the Azerbaijan flora

Artemisia plants and foliaceous lichens are known to be capable of accumulating heavy metals (HM) from soil and air. These plant species are widespread on polluted sites of Azerbaijan. However, so far their capacity to accumulate HM in their shoots and roots has not been tested. Three Artemisia and two lichen species were collected from different contaminated sites of Azerbaijan. Plant and surface soil samples were measured for Cd, Cu, Pb, Ni and Zn concentrations by ICP-AES.The results indicated that among the Artemisia species A. scoparia showed the best HM accumulation properties. Lichen species were also distinguished by very high amounts of HM in their biomass, while in surrounding soil samples HM concentrations had higher contents than the soils occupied only with Artemisia species.The results indicate that on contaminated sites Artemisia and lichens accumulated metals in their biomass without toxicity symptoms. Taking large biomass and high adaptation ability into account, A. scoparia represents a good tool for a phytoremediation approach on polluted soils

Implantable Medical Devices; Networking Security Survey

Abstract The industry of implantable medical devices (IMDs) is constantly evolving, which is dictated by the pressing need to comprehensively address new challenges in the healthcare field. Accordingly, IMDs are becoming more and more sophisticated. Not long ago, the range of IMDs' technical capacities was expanded, making it possible to establish Internet connection in case of necessity and/or emergency situation for the patient. At the same time, while the web connectivity of today's implantable devices is rather advanced, the issue of equipping the IMDs with sufficiently strong security system remains unresolved. In fact, IMDs have relatively weak security mechanisms which render them vulnerable to cyber-attacks that compromise the quality of IMDs' functionalities. This study revolves around the security deficiencies inherent to three types of sensor-based medical devices; biosensors, insulin pump systems and implantable cardioverter defibrillators. Manufacturers of these devices should take into consideration that security and effectiveness of the functionality of implants is highly dependent on the design. In this paper, we present a comprehensive study of IMDs' architecture and specifically investigate their vulnerabilities at networking interface

TP53 mutations, amplification of P63 and expression of cell cycle proteins in squamous cell carcinoma of the oesophagus from a low incidence area in Western Europe

In Europe, high incidence rates of oesophageal squamous cell carcinoma (SCCE) are observed in western France (Normandy and Brittany) and in north-eastern Italy. Analysis of TP53 mutations in tumours from these regions has shown a high prevalence of mutations at A:T basepairs that may result from DNA damage caused by specific mutagens. However, the spectrum of TP53 mutations in regions of low incidence is unknown. We report here TP53 mutation analysis in 33 SCCE collected in Lyon, an area of low incidence. These tumours were also examined for MDM2 and P63 amplification, and for expression of p16INK4a/CDKN2a, cyclin E, p27Kipland Cox2. TP53 mutations were detected in 36% of the cases (12/33). In contrast with regions of high incidence, the mutation spectrum did not show a high prevalence of mutations at A:T base pairs. P63 was amplified in 5/32 cases tested (15.5%). No amplification of MDM2 was found. Expression studies revealed frequent loss of p16INK4a/CDKN2a(46%) and p27Kipl(25%) expression, and frequent overexpression of Cyclin E (70%) and Cox2 (42%). Overall, these results indicate that in Europe, SCCE from areas of high and low incidence present a similar pattern of molecular alterations but differ by the type of TP53 mutations. © 2001 Cancer Research Campaign http://www.bjcancer.co

Gene expression changes associated with Barrett's esophagus and Barrett's-associated adenocarcinoma cell lines after acid or bile salt exposure

<p>Abstract</p> <p>Background</p> <p>Esophageal reflux and Barrett's esophagus represent two major risk factors for the development of esophageal adenocarcinoma. Previous studies have shown that brief exposure of the Barrett's-associated adenocarcinoma cell line, SEG-1, or primary cultures of Barrett's esophageal tissues to acid or bile results in changes consistent with cell proliferation. In this study, we determined whether similar exposure to acid or bile salts results in gene expression changes that provide insights into malignant transformation.</p> <p>Methods</p> <p>Using previously published methods, Barrett's-associated esophageal adenocarcinoma cell lines and primary cultures of Barrett's esophageal tissue were exposed to short pulses of acid or bile salts followed by incubation in culture media at pH 7.4. A genome-wide assessment of gene expression was then determined for the samples using cDNA microarrays. Subsequent analysis evaluated for statistical differences in gene expression with and without treatment.</p> <p>Results</p> <p>The SEG-1 cell line showed changes in gene expression that was dependent on the length of exposure to pH 3.5. Further analysis using the Gene Ontology, however, showed that representation by genes associated with cell proliferation is not enhanced by acid exposure. The changes in gene expression also did not involve genes known to be differentially expressed in esophageal adenocarcinoma. Similar experiments using short-term primary cultures of Barrett's esophagus also did not result in detectable changes in gene expression with either acid or bile salt exposure.</p> <p>Conclusion</p> <p>Short-term exposure of esophageal adenocarcinoma SEG-1 cells or primary cultures of Barrett's esophagus does not result in gene expression changes that are consistent with enhanced cell proliferation. Thus other model systems are needed that may reflect the impact of acid and bile salt exposure on the esophagus <it>in vivo</it>.</p

Oncogene Activation Induces Metabolic Transformation Resulting in Insulin-Independence in Human Breast Cancer Cells

Normal breast epithelial cells require insulin and EGF for growth in serum-free media. We previously demonstrated that over expression of breast cancer oncogenes transforms MCF10A cells to an insulin-independent phenotype. Additionally, most breast cancer cell lines are insulin-independent for growth. In this study, we investigated the mechanism by which oncogene over expression transforms MCF10A cells to an insulin-independent phenotype. Analysis of the effects of various concentrations of insulin and/or IGF-I on proliferation of MCF10A cells demonstrated that some of the effects of insulin were independent from those of IGF-I, suggesting that oncogene over expression drives a true insulin-independent proliferative phenotype. To test this hypothesis, we examined metabolic functions of insulin signaling in insulin-dependent and insulin-independent cells. HER2 over expression in MCF10A cells resulted in glucose uptake in the absence of insulin at a rate equal to insulin-induced glucose uptake in non-transduced cells. We found that a diverse set of oncogenes induced the same result. To gain insight into how HER2 oncogene signaling affected increased insulin-independent glucose uptake we compared HER2-regulated gene expression signatures in MCF10A and HER2 over expressing MCF10A cells by differential analysis of time series gene expression data from cells treated with a HER2 inhibitor. This analysis identified genes specifically regulated by the HER2 oncogene, including VAMP8 and PHGDH, which have known functions in glucose uptake and processing of glycolytic intermediates, respectively. Moreover, these genes specifically implicated in HER2 oncogene-driven transformation are commonly altered in human breast cancer cells. These results highlight the diversity of oncogene effects on cell regulatory pathways and the importance of oncogene-driven metabolic transformation in breast cancer