Inter‐Individual versus

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Extraction of neuroprotective compounds from Eucalyptus leaves using green technologies. Ternary mixture design

Resumen del trabajo presentado al 19th European Meeting on Supercritical Fluids: Budapest, Hungary, 21–24 May 2023.Authors thank projects PID2020-113050RB-I00, and PDC2021-120814-I00 funded by MCIN/AEI /10.13039/501100011033 and The European Union Next GenerationEU/ PRTR, also INCGLO0019 (Bioprospection of local agricultural resources, a way to achieve the Objectives of Sustainable development) and COOPA22033 (Bioprospecting Algerian native plants by means of sustainable extraction techniques to find bioactive compounds) both financed by the CSIC.N

Chemical composition, antioxidant and cytotoxic activities of Onopordum acanthium L. Crude oil and defatted meal

In the present study, the seed oil from Onopordum acanthium. L and methanolic extract of the defatted meal were evaluated for their cytotoxic effect and antioxidant potential. The phenolic composition of the defatted meal was determined with RP-HPLC-UV and tocopherols levels in the crude oil were also assessed. Overall, the methanolic extract exhibited the best amount of total phenolics and flavonoids content (78.06±0.55 mg GAE/g on DW basis and 20.38±0.21 mg RE/g on DW basis, respectively).Seven phenolic compounds were identified with cinnamic acids as the major components (67.2%) and epicatechin was the dominant flavan-3-ol. Additionally, three individual tocopherols were determined and α-tocopherol was the main isomer (914.8±0.02mg/kg). Data of antioxidant performance showed superior antioxidant capacity in methanol extract. Moreover methanolic extract from defatted seeds was more active towards HepG2 cancer cells than the crude oil(IC50 of 44.05±3.54µg/ml) and showed less cytotoxicity towards AML12 normal hepatocytes in comparison to doxorubicin indicating at least some tumor-specific action