Poster: Towards Federated LLM-Powered CEP Rule Generation and Refinement

In traditional event processing systems, patterns representing situations of interest are typically defined by domain experts or learned from historical data. These approaches often make rule generation reactive, time-consuming, and susceptible to human error. In this paper, we propose and investigate the integration of large language models (LLMs) to automate and accelerate query translation and rule generation in event processing systems. Furthermore, we introduce a federated learning schema to refine the initially generated rules by examining them over distributed event streams, ensuring greater accuracy and adaptability.Preliminary results demonstrate the potential of LLMs as a key component in proactively expediting the autonomous rule-generation process. Moreover, our findings suggest that employing customized prompt engineering techniques can further enhance the quality of the generated rules

Poster: Towards Federated LLM-Powered CEP Rule Generation and Refinement

In traditional event processing systems, patterns representing situations of interest are typically defined by domain experts or learned from historical data. These approaches often make rule generation reactive, time-consuming, and susceptible to human error. In this paper, we propose and investigate the integration of large language models (LLMs) to automate and accelerate query translation and rule generation in event processing systems. Furthermore, we introduce a federated learning schema to refine the initially generated rules by examining them over distributed event streams, ensuring greater accuracy and adaptability.Preliminary results demonstrate the potential of LLMs as a key component in proactively expediting the autonomous rule-generation process. Moreover, our findings suggest that employing customized prompt engineering techniques can further enhance the quality of the generated rules

Lipidomic Profiling of Murine Macrophages Treated with Fatty Acids of Varying Chain Length and Saturation Status

Macrophages are abundant within adipose tissue depots where they are exposed to fatty acids, leading to lipid accumulation. Herein, we have determined the effects of various fatty acids on the macrophage lipidome. Using targeted mass-spectrometry, we were able to detect 641 individual lipid species in primary murine macrophages treated with a variety of saturated fatty acids and an un-saturated fatty acid, either alone or in combination. The most pronounced effects were observed for the long-chain saturated fatty acid palmitate, which increased the total abundance of numerous classes of lipids. While other medium- and long-chain saturated fatty acids, as well as the long-chain unsaturated fatty acid, had less pronounced effects on the total abundance of specific lipid classes, all fatty acids induced marked alterations in the abundance of numerous lipid species within given lipid classes. Fatty acid treatment markedly altered overall phospholipid saturation status; these effects were most pronounced for phosphatidylcholine and ether-phosphatidylcholine lipid species. Finally, treatment of macrophages with either palmitate or stearate in combination with oleate prevented many of the changes that were observed in macrophages treated with palmitate or stearate alone. Collectively, our results reveal substantial and specific remodelling of the macrophage lipidome following treatment with fatty acids

Tuning the functional properties of lignocellulosic films by controlling the molecular and supramolecular structure of lignin

| openaire: EC/H2020/720303/EU//Zelcor Funding Information: This work was funded by the Bio Based Industry Joint Undertaking under the European Union's Horizon 2020 research and innovation programme within the Zelcor project (under the grant number No 720303 ), part of the COFILI project (grant number D201550245 ) for AFM measurements funded by the Grand Est Region and the European FEDER Programme and the Lignoxyl project for EPR measurements supported by the Agence Nationale de la Recherche (ANR) through the Carnot Institutes 3BCAR ( www.3bcar.fr ) and Qualiment ( https://qualiment.fr/ ) (no. 3 no. 19-CARN-001-01 and no. 16-CARN 001-01). The EPR data in this manuscript were obtained using equipment supported jointly by the French National Ministry of Research (PPF IRPE), the “Fondation pour la Recherche Médicale” (FRM DGE20061007745), and the CNRS (Department of Chemistry and Life Sciences). The IJPB benefits from the support of the LabEx Saclay Plant Sciences-SPS (ANR-10-LABX-552 0040-SPS). Funding Information: This work was funded by the Bio Based Industry Joint Undertaking under the European Union's Horizon 2020 research and innovation programme within the Zelcor project (under the grant number No 720303), part of the COFILI project (grant number D201550245) for AFM measurements funded by the Grand Est Region and the European FEDER Programme and the Lignoxyl project for EPR measurements supported by the Agence Nationale de la Recherche (ANR) through the Carnot Institutes 3BCAR (www.3bcar.fr) and Qualiment (https://qualiment.fr/) (no. 3 no. 19-CARN-001-01 and no. 16-CARN 001-01). The EPR data in this manuscript were obtained using equipment supported jointly by the French National Ministry of Research (PPF IRPE), the ?Fondation pour la Recherche M?dicale? (FRM DGE20061007745), and the CNRS (Department of Chemistry and Life Sciences). The IJPB benefits from the support of the LabEx Saclay Plant Sciences-SPS (ANR-10-LABX-552 0040-SPS). Publisher Copyright: © 2021 The Authors Copyright: Copyright2021 Elsevier B.V., All rights reserved.This study investigated the relationships between lignin molecular and supramolecular structures and their functional properties within cellulose-based solid matrix, used as a model biodegradable polymer carrier. Two types of derivatives corresponding to distinct structuration levels were prepared from a single technical lignin sample (PB1000): phenol-enriched oligomer fractions and colloidal nanoparticles (CLP). The raw lignin and its derivatives were formulated with cellulose nanocrystals or nanofibrils to prepare films by chemical oxidation or pressure-assisted filtration. The films were tested for their water and lignin retention capacities, radical scavenging capacity (RSC) and antimicrobial properties. A structural investigation was performed by infrared, electron paramagnetic resonance spectroscopy and microscopy. The composite morphology and performance were controlled by both the composition and structuration level of lignin. Phenol-enriched oligomers were the compounds most likely to interact with cellulose, leading to the smoothest film surface. Their RSC in film was 4- to 6-fold higher than that of the other samples. The organization in CLP led to the lowest RSC but showed capacity to trap and stabilize phenoxy radicals. All films were effective against S. aureus (gram negative) whatever the lignin structure. The results show the possibility to tune the performances of these composites by exploiting lignin multi-scale structure.Peer reviewe

Cholesterol transport between red blood cells and lipoproteins contributes to cholesterol metabolism in blood

Lipoproteins play a key role in transport of cholesterol to and from tissues. Recent studies have also demonstrated that red blood cells (RBCs), which carry large quantities of free cholesterol in their membrane, play an important role in reverse cholesterol transport. However, the exact role of RBCs in systemic cholesterol metabolism is poorly understood. RBCs were incubated with autologous plasma or isolated lipoproteins resulting in a significant net amount of cholesterol moved from RBCs to HDL, while cholesterol from LDL moved in the opposite direction. Furthermore, the bi-directional cholesterol transport between RBCs and plasma lipoproteins was saturable and temperature-, energy-, and time-dependent, consistent with an active process. We did not find LDLR, ABCG1, or scavenger receptor class B type 1 in RBCs but found a substantial amount of ABCA1 mRNA and protein. However, specific cholesterol efflux from RBCs to isolated apoA-I was negligible, and ABCA1 silencing with siRNA or inhibition with vanadate and Probucol did not inhibit the efflux to apoA-I, HDL, or plasma. Cholesterol efflux from and cholesterol uptake by RBCs from Abca1 +/+ and Abca1 -/- mice were similar, arguing against the role of ABCA1 in cholesterol flux between RBCs and lipoproteins. Bioinformatics analysis identified ABCA7, ABCG5, lipoprotein lipase, and mitochondrial translocator protein as possible candidates that may mediate the cholesterol flux. Together, these results suggest that RBCs actively participate in cholesterol transport in the blood, but the role of cholesterol transporters in RBCs remains uncertain