Phylogenomic analysis reveals extensive phylogenetic mosaicism in the Human GPCR Superfamily

A novel high throughput phylogenomic analysis (HTP) was applied to the rhodopsin G-protein coupled receptor (GPCR) family. Instances of phylogenetic mosaicism between receptors were found to be frequent, often as instances of correlated mosaicism and repeated mosaicism. A null data set was constructed with the same phylogenetic topology as the rhodopsin GPCRs. Comparison of the two data sets revealed that mosaicism was found in GPCRs in a higher frequency than would be expected by homoplasy or the effects of topology alone. Various evolutionary models of differential conservation, recombination and homoplasy are explored which could result in the patterns observed in this analysis. We find that the results are most consistent with frequent recombination events. A complex evolutionary history is illustrated in which it is likely frequent recombination has endowed GPCRs with new functions. The pattern of mosaicism is shown to be informative for functional prediction for orphan receptors. HTP analysis is complementary to conventional phylogenomic analyses revealing mosaicism that would not otherwise have been detectable through conventional phylogenetics

Appropriate technology for Aboriginal Enterprise Development

RADG has been developing appropriate health technology for use in remote communities in Australia. The greatest need for these technologies has been in Aboriginal communities. In developing appropriate technical artifacts, RADG has confronted two problems. Firstly we require good contact with remote communities for consultation and feedback. Secondly, part of making artifacts appropriate for under-developed countries or regions, is the need to include employment and self-determination as part of the benefits of a technology

Biological functions of CDK5 and potential CDK5 targeted clinical treatments.

Cyclin dependent kinases are proline-directed serine/threonine protein kinases that are traditionally activated upon association with a regulatory subunit. For most CDKs, activation by a cyclin occurs through association and phosphorylation of the CDK\u27s T-loop. CDK5 is unusual because it is not typically activated upon binding with a cyclin and does not require T-loop phosphorylation for activation, even though it has high amino acid sequence homology with other CDKs. While it was previously thought that CDK5 only interacted with p35 or p39 and their cleaved counterparts, Recent evidence suggests that CDK5 can interact with certain cylins, amongst other proteins, which modulate CDK5 activity levels. This review discusses recent findings of molecular interactions that regulate CDK5 activity and CDK5 associated pathways that are implicated in various diseases. Also covered herein is the growing body of evidence for CDK5 in contributing to the onset and progression of tumorigenesis

Monadicity of the Bousfield-Kuhn functor

We consider the localization of the $\infty$-category of spaces at the $v_n$-periodic equivalences, the case $n=0$ being rational homotopy theory. We prove that this localization is for $n\geq 1$ equivalent to algebras over a certain monad on the $\infty$-category of $T(n)$-local spectra. This monad is built from the Bousfield--Kuhn functor

H2S paper strip method - A bacteriological test for faecal coliforms in drinking water at various temperatures

Epidemics arising from waterborne diseases are a global health problem. Faecal contamination of drinking water is the main cause of these outbreaks. According to WHO (1996) for drinking water to be safe, a 100 ml sample should not contain any coliform bacteria. The standard methods currently used for routine testing have many limitations especially when applied in remote areas. The H2S method has been developed as an on-site, inexpensive and easy to use method to test drinking water for remote and rural areas. The present work analyses the reliability of the H2S method for detecting faecal contamination in drinking water. The minimum level of faecal coliforms that could be detected and the incubation period required at various levels of contamination were studied. The range of temperatures at which the method was effective and the incubation period required at various temperatures were also determined. The H2S method was found to be able to detect contamination down to a level of 1 CFU/100 ml of coliform bacteria. Although the H2S method could be used at a temperature range of 20 to 44oC, temperatures between 28 to 37oC gave faster results. An incubation period of only 24 hours was required at 37oC, which was found to be the most suitable incubation temperature. The incubation period increased with a decrease or increase in temperature

On-site wastewater reuse technology options in Australia

Domestic wastewater reuse is currently not permitted anywhere in Australia but is widely supported by the community, promoted by researchers, and improvised by up to 20% of householders. Its widespread implementation will make an enormous contribution to the sustainability of water resources. Integrated with other strategies in the outdoor living environment of settlements in arid lands great benefit will be derived. This paper describes six options for wastewater reuse under research by the Remote Area Developments Group (RADG) at Murdoch University and case studies are given where productive use is being made for revegetation and food production strategies at household and community scales. Pollution control techniques, public health precautions and maintenance requirements are described. The special case of remote Aboriginal communities is explained where prototype systems have been installed by RADG to generate windbreaks and orchards. New Australian design standards and draft guidelines for domestic greywater reuse produced by the Western Australian state government agencies for mainstream communities are evaluated. It is recommended that dry composting toilets be coupled with domestic greywater reuse and the various types available in Australia are described. For situations where only the flushing toilet will suffice the unique "net composting" system can be used and this also is described. A vision for household and community-scale on-site application is presented

Financial Advisors' Role in Influencing Social Security Claiming

For millions of Americans, financial advisors are a trusted source of financial and retirement preparation information. This includes providing advice and information on Social Security benefits, a critical component of most Americans’ retirement finances. To gain greater insight into what financial advisors say to their clients about Social Security, an online survey of over 400 professional financial advisors was conducted in the Spring of 2011. The results reveal that a majority of advisors believe that they are responsible for educating their clients on the role Social Security will play in their retirement income. Moreover, advisors have the ability to influence their clients’ decisions about when to claim their Social Security retirement benefits. Three-quarters advise the majority of their clients on when to claim. In addition, the study finds that the Social Security Administration (SSA) is the leading and preferred source of information and education for financial advisors and their clients. Over half of advisors say it is a major source of Social Security-related information, more than any other source. However, advisors are critical of the job SSA does in educating advisors and the public, and are interested in additional resources from the Agency. Financial advisors also indicate that the financial services companies they work with could improve their communication and education efforts as it relates to Social Security. The research findings uncover a need for improved methods of educating and disseminating information to financial advisors and the public on Social Security.