The impact of protein quantity during energy restriction on genome-wide gene expression analysis in adipose tissue of obese humans

BACKGROUND: Overweight and obesity is a growing health problem worldwide. The most effective strategy to reduce weight is energy restriction (ER). ER has been shown to be beneficial in disease prevention and it reduces chronic inflammation. Recent studies suggest that reducing the protein quantity of a diet contributes to the beneficial effects by ER. The organ most extensively affected during ER is white adipose tissue (WAT). OBJECTIVE: The first objective was to assess changes in gene expression between a high protein diet and a normal protein diet during ER. Secondly, the total effect of ER on changes in gene expression in WAT was assessed. METHODS: In a parallel double-blinded controlled study, overweight older participants adhered to a 25% ER diet, either combined with high protein intake (HP-ER, 1.7 g/kg per day), or with normal protein intake (NP-ER, 0.9 g/kg per 40 day) for 12 weeks. From 10 HP-ER participants and 12 NP-ER participants subcutaneous WAT biopsies were collected before and after the diet intervention. Adipose tissue was used to isolate total RNA and to evaluate whole genome gene expression changes upon a HP-ER and NP-ER diet. RESULTS: A different gene expression response between HP-ER and NP-ER was observed for 530 genes. After NP-ER a downregulation in expression of genes linked to immune cell infiltration, adaptive immune response, and inflammasome was found whereas no such effect was found after HP-ER. HP-ER resulted in upregulation in expression of genes linked to cell cycle, GPCR signalling, olfactory signalling and nitrogen metabolism. Upon 25% ER, gene sets related to energy metabolism and immune response were decreased. CONCLUSIONS: Based on gen e expression changes, we concluded that consumption of normal protein quantity compared to high protein quantity during ER has a more beneficial effect on inflammation-related gene expression in WAT

Experimental investigation on the effect of the duct geometrical parameters on the performance of a ducted wind turbine

This paper reports an experimental investigation on the effect of the duct geometry on the aerodynamic performance of an aerofoil shaped ducted wind turbine (DWT). The tested two-dimensional model is composed of an aerofoil equipped with pressure taps and a uniform porous screen. The experimental setup is based on the assumption that the duct flow is axisymmetric and the rotor can be simulated as an actuator disc. Firstly, different tip clearances between the screen and the aerofoil are tested to point out the influence of this parameter on the DWT performance in terms of aerofoil pressure distribution, aerofoil lift and flow field features at the duct exit area. Then, the combined effect of tip clearance, of the angle of attack and of the screen position along the aerofoil chord is evaluated through a Design of Experiments (DoE) based approach. The analysis shows that, among the analysed range of design factor variation, increasing angle of attack and the tip clearance leads to a beneficial effect on the lift and back-pressure coefficients, while they show a poor dependence upon the screen axial position. Finally, the configuration characterized by the maximum value of all three main factors (15 degree of angle of attack, 5% of tip clearance and 30% backward to the nozzle plane), has the best values of lift coefficient and back-pressure coefficient.Wind Energ