Tumor Susceptibility Gene 101 (TSG101) Is a Novel Binding-Partner for the Class II Rab11-FIPs

The Rab11-FIPs (Rab11-family interacting proteins; henceforth, FIPs) are a family of Rab11a/Rab11b/Rab25 GTPase effector proteins implicated in an assortment of intracellular trafficking processes. Through proteomic screening, we have identified TSG101 (tumor susceptibility gene 101), a component of the ESCRT-I (endosomal sorting complex required for transport) complex, as a novel FIP4-binding protein, which we find can also bind FIP3. We show that α-helical coiled-coil regions of both TSG101 and FIP4 mediate the interaction with the cognate protein, and that point mutations in the coiled-coil regions of both TSG101 and FIP4 abrogate the interaction. We find that expression of TSG101 and FIP4 mutants cause cytokinesis defects, but that the TSG101-FIP4 interaction is not required for localisation of TSG101 to the midbody/Flemming body during abscission. Together, these data suggest functional overlap between Rab11-controlled processes and components of the ESCRT pathway

Metabotropic action of postsynaptic kainate receptors triggers hippocampal long-term potentiation

Long-term potentiation (LTP) in the rat hippocampus is the most extensively studied cellular model for learning and memory. Induction of classical LTP involves an NMDA receptor- and calcium-dependent increase in functional synaptic AMPA receptors mediated by enhanced recycling of internalized AMPA receptors back to the postsynaptic membrane. Here we report a novel, physiologically relevant NMDA receptor-independent mechanism that drives increased AMPA receptor recycling and LTP. This pathway requires the metabotropic action of kainate receptors and activation of G-protein, protein kinase C and phospholipase C. Like classical LTP, kainate receptor-dependent LTP recruits recycling endosomes to spines, enhances synaptic recycling of AMPA receptors to increase their surface expression and elicits structural changes in spines, including increased growth and maturation. These data reveal a new and previously unsuspected role for postsynaptic kainate receptors in the induction of functional and structural plasticity in the hippocampus

Enea Vico, verissimo oracolo degli antiquari, tra studi numismatici e interesse per l'antico nel Cinquecento

Dottorato di ricerca in antichita' classiche in Italia e loro fortuna. 7. ciclo. A. a. 1991-94. Coordinatore Antonio GiulianoConsiglio Nazionale delle Ricerche - Biblioteca Centrale - P.le Aldo Moro, 7, Rome; Biblioteca Nazionale Centrale - P.za Cavalleggeri, 1, Florence / CNR - Consiglio Nazionale delle RichercheSIGLEITItal

A survey of the European valvatiform hydrobiid genera with special reference to Hauffenia Pollonera, 1898 (Gastropoda: Hydrobiidae)

All the taxa of genus and species groups introduced for the European valvatiform hydrobiids are analyzed, and if possible revised. All the type species have been redescribed on the basis of new anatomical studies or data in the literature when spirit specimens were not available. This enabled the following taxa. to be recognized as junior synonyms of Hauffenia Pollonera, 1898 (type species Horatia (Hauffenia) tellinii Pollonera, 1898): Erythropomatiana Radoman, 1978 (type species Valvata erythropomatia Hauffen, 1856); Lobaunia Haase, 1993 (type species: Lobaunia danubialis Haase, 1993); Neohoratia Schutt, 1961 (type species Valvata (?) subpiscinalis Kuscer, 1932); and Vrania Radoman, 1978 (type species Valvata wagneri Kuscer, 1928). Of the species currently assigned to Hauffenia, only a few from the eastern Alps and Balkans actually belong to it: H. erythropomatia (Hauffen, 1856); H. kerschneri (Zimmermann, 1930); H. media Bole, 1961; H. subcarinata Bole & Velkovrh, 1987; H. subpiscinalis ( Kuscer, 1932); H. tellinii (Pollonera, 1898) (with H. michleri Kuscer, 1932, as junior synonym); H. tovunica Radoman, 1978; H. wagneri (Kuscer, 1928); and H. wienerwaldensis Haase, 1992. Each is redescribed. Many "Hauffenia" species from the Balkans remain of uncertain generic status due to the absence, or incompleteness of anatomical data. However, Horatia (Hauffenia) raehlei Schutt, 1980, is placed in the genus Fissuria Boeters, 1981, on the basis of its anatomy. After anatomical study, most of the French "Hauffenia" have been assigned to Islamla Radoman, 1973a, and tentatively to the following species: I. minuta (Draparnaud, 1805), I. globulina (Paladilhe, 1866), I. spirata (Bernasconi, 1985), and I. consolationis (Bernasconi, 1985). Anatomical study of the French "Horatia" exilis (Paladilhe, 1867) supports its allocation to a new genus: Heraultia, n. gen. The Spanish species currently assigned to "Horatia" or "Neohoratia" do not belong to these genera. Some of them, namely "N." ateni (Boeters, 1969), "N." globulus globulus (Bofill, 1909), and "N." g. Iagari (Altimira, 1960), belong to Islamia. Diagnoses and keys are provided for the European valvatiform hydrobiid genera and for the species of Hauffenia, together with tables summarising their taxonomic status and distribution

Hydrobia Hartmann, 1821 and Cyclostoma acutum Draparnaud, 1805 (currently Hydrobia acuta; Mollusca, Gastropoda): proposed conservation by replacement of the lectotype of H. acuta with a neotype; Ventrosia Radoman, 1977: proposed designation of Turbo ventrosus Montagu, 1803 as the type species; and HYDROBIINA Mulsant, 1844 (Insecta, Coleoptera): proposed emendation of spelling to HYDROBIUSINA, so removing the homonymy with HYDROBIIDAE Troschel, 1857 (Mollusca)

Volume: 55Start Page: 139End Page: 14