Evaluation of Physical Exercise'S Effects on Low Back Pain Among University Students With Short Form-36 and Oswestry Disability Index

DergiPark: 762681tmsjAims: This study aims to investigate the main effects of physical exercise on lower back pain with the Short Form-36 Health Survey and Oswestry Disability Index scores. Methods: University students between 18-25 years old who suffer from low back pain were enrolled in the study. Short Form-36 Health Survey and Oswestry Disability Index were used as the reference scales of lower back pain and quality of life, respectively. There are some questions in the questionnaire to scale the average comfort of the place they sleep and sit during the day, which is the result of the Personal Comfort Score. The Mann-Whitney U test was used for non-normal distributed variables. Correlation and Linear Regression were used to analyze data. SPSS 25.0 was used for all statistical analysis. Results: A total of 139 university students (94 females, 45 males) were included in the study. The median age was 20 years (IQR=2) for female students and 20 years (IQR=1) for male students. Oswestry Disability Index scores of fe- male students were higher, while Short Form-36 scores were lower than the male students. Male students' physical functioning scores were higher than female students. Each one-unit increase in Personal Comfort Score is associated with an increase in the rate between the energy/fatigue (3.34 units). Body Mass Index considerably affected the pain and Oswestry Disability Score, an increase in one unit of baseline BMI upsurged the Short Form-36 pain score to 0.13, and Oswestry disability score to 0.55. Conc- lusion: In our study, Body Mass Index is found to be associated with Oswestry Disability Index and Short Form-36 score, which are used for the severity of low back pain and defining the life quality and of patients. PCS had a positive correlation between energy/fatigue. In addition, There was a positive correlation between physical exercise and general health score

Bakteriyel Vajinal Mikrobiyotanın Metagenomik Yaklaşımla Tanımlanması

Amaç: Bu çalışmanın amacı, alem düzeyinden tür düzeyine kadar farklı taksonomik seviyelerde yüksek verimli yeni nesil dizileme ve metagenomik yaklaşım kullanarak 38 Türk kadınının vajinal bakteriyel mikrobiyotasını belirlemektir. Gereç ve Yöntem: Yeditepe Üniversitesi Hastanesi Kadın Hastalıkları ve Doğum Kliniği’nde Haziran 2021’de DNA/RNA koruma toplama tüplerine vajinal sürüntü örnekleri (n=38) alındı ve ZymoBIOMICS DNA miniprep kiti ile DNA ekstraksiyonu yapıldı. Hastaların yaşı, medeni durumu, ön tanı ve anamnez durumu ile ilgili bilgiler toplandı. Vajinal mikrobiyotayı belirlemek için 16S rRNA amplikon DNA dizilimi kullanılarak metagenomik bir yaklaşım uygulandı. Bulgular: Vajinal örneklerde baskın filum Firmicutes’i Proteobacteria, Actinobacteria, Tenericutes, Fusobacteria ve Synergistetes izledi. Lactobacillus en fazla bulunan “cins düzeyinde bakteri” olup onu Prevotella, Enterobacter, Gardnerella ve Dialister izledi. Vajinal sürüntü örneklerinde tür düzeyinde Lactobacillus iners baskın bulundu, bunu Gardnerella vaginalis, Enterobacter tabaci, Prevotella timonensis, Prevotella bivia ve Lactobacillus jensenii izledi. Kanonik uyum analizi (CCA), filum düzeyinde Proteobacteria ve Fusobacteria’nın en yüksek yüzdelerle evli/bekar değişkeni ile ilişkili olduğunu, ancak Actinobacteria ve Tenericutes’in yaş değişkeni ile ilişkili olduğunu gösterdi. Campylobacter, Atopobium, Enterobacter ve Lactococcus en yüksek yüzdelerle evli/bekar değişkeni ile ilişkili bulunurken, Anaerococcus, Streptococcus, Sutterella ve Veillonella en yüksek yüzdelerle yaşla ilişkili bulundu. Ayrıca, CCA, Campylobacter ureolyticus, Lb. jensenii ve Atopobium vajinae türlerinin evli/bekar değişkeni ile en yüksek yüzdelerle ilişkilendirirken, Lactobacillus johnsonii ve G. vaginalis en yüksek yüzdelerle yaş değişkeninde ilişkili bulundu. Sonuç: Vajinal hastalıklar hala önemli bir halk sağlığı sorunudur. Son yıllarda teknolojik gelişmeler sayesinde daha derinlemesine çalışılan vajinal mikrobiyotanın sanıldığından daha karmaşık olduğu keşfedilmiştir. Bu bulguları doğrulamak ve geliştirmek için daha fazla hasta araştırmasına ihtiyaç vardır

Improved abnormal brain electrical activity via HMG-CoA reductase inhibitor rosuvastatin and the role of PI3K/Akt pathway

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Label-free surface enhanced raman spectroscopy for cancer detection

Simple Summary Blood is considered a rich reservoir of biomarkers for disease diagnosis. Surface-enhanced Raman scattering (SERS) is known for its high sensitivity and has been successfully employed to differentiate blood samples from cancer patients versus healthy individuals. Different from previous reports, this study aims at investigating the reliability of the observed results by varying several parameters influencing the observed spectra. Thus, blood taken from 30 healthy individuals as the control group, 30 patients with different types of cancers, and 15 patients with various types of chronic diseases were used in the study. The results revealed that spectral differences in the cancer group was directly related to the presence of cancer-related biomarkers. Although data were obtained from only small group of patients, the recorded sensitivity and specificity values clearly show the power of the technique to detect cancer. Blood is a vital reservoir housing numerous disease-related metabolites and cellular components. Thus, it is also of interest for cancer diagnosis. Surface-enhanced Raman spectroscopy (SERS) is widely used for molecular detection due to its very high sensitivity and multiplexing properties. Its real potential for cancer diagnosis is not yet clear. In this study, using silver nanoparticles (AgNPs) as substrates, a number of experimental parameters and scenarios were tested to disclose the potential for this technique for cancer diagnosis. The discrimination of serum samples from cancer patients, healthy individuals and patients with chronic diseases was successfully demonstrated with over 90% diagnostic accuracies. Moreover, the SERS spectra of the blood serum samples obtained from cancer patients before and after tumor removal were compared. It was found that the spectral pattern for serum from cancer patients evolved into the spectral pattern observed with serum from healthy individuals after the removal of tumors. The data strongly suggests that the technique has a tremendous potential for cancer detection and screening bringing the possibility of early detection onto the table