Comparison of Spermac and Diff-quik staining methods in the assessment of sperm morphology in asthenozoopermia cases [Asthenozoospermia olgularinda sperm morfolojisi degerlendirmede Spermac ve Diff-quik boya yöntemlerinin karşilaştirilmasi]

Sperm motility problems (Asthenozoopermia) is one of the major causes of male infertility. In cases with asthenozoopermia infertility increases to a greater extent when sperm morphology disorders are added to coexisting motility disorders. A total of 65 males (21 males with sperm motility disorder and 44 males without this disorder) who referred to the in vitro fertilization unit of Dokuz Eylul University Medical School between November 15, 2004 and April 5, 2005 were included in the present study. Spermac and Diff-quik staining methods were used in the assessment of sperm morphology in all semen samples. Test results showed consistency with both staining methods on 55 semen samples examined. Sperm morphology was normal in 32 semen samples with consistent test results by both methods, while the percentage of morphology was low in 23 cases. In 7 of remaining 10 cases, the sperm morphology was in the normal range with Diff-quik staining method while an abnormal percentage was detected with Spermac method. In 3 cases the sperm morphology was within normal limits with Spermac staining method while Diff-quik staining method demonstrated abnormal sperm morphology. Statistical analysis with Mc Nemar test demonstrated that both staining methods were reliable. Since neither of the two methods used in the evaluation of sperm morphology has proven superiority to the other, a cost reduction may be provided by choosing the economical one. © Gülhane Askeri Tip Akademisi 2008

Comparison of eosin and HOS test methods in assessment of sperm viability in asthenozoospermia cases [Astenozoospermi olgularinda semende canlilik testinin degerlendirilmesinde eozin ve HOS testlerinin karşilaştirilmasi]

Objective: This study investigated whether one of the two viability tests is superior to the other in cases with asthenozoospermia (reduced sperm motility) in which the rate of immotile spermatozoa exceeded 50%. Materials and Methods: A total of 21 male patients who visited the IVF (in vitro fertilization) unit of the Department of Obstetrics and Gynecology of Dokuz Eylül University Medical Faculty were included in the study. Semen samples were subjected to both macroscopic and microscopic examinations. Makler counting chamber was used for the sperm motility and concentration analyses. All semen samples were subjected to eosin viability test and HOS (hypo-osmolar swelling) test. The result of the eosin test was accepted as normal in the case of presence of 75% or more unstainable spermatozoa in a semen sample. The result of the HOS test was accepted as normal if swelling behavior were observed in more than 60% of the spermatozoa tails in a semen sample. Results: Both tests yielded normal results in 10 cases, while the test results were found to be abnormal in 4 of the cases. In 7 cases, the HOS test yielded abnormal results, while the results of the eosin test were found to be normal. The binomial (sign test) statistical evaluation revealed that the two tests have no superiority over one another (p>0.05). Discussion: The fact that the HOS test as a viability test yielded very similar results when compared to the eosin test demonstrates the significance of the HOS test in the sense that it is economic

Comparison of the effects of 2 different doses of remifentanil infusion for sedation during in-vitro fertilization procedure.

Objectives: To compare the sedation level, hemodynamic effects, patient and physician satisfactions following sedation achieved by 2 different doses of remifentanil (R) infusion with additional bolus infusions of propofol for in vitro fertilization (IVF) procedure