6 research outputs found

    Vibration Signal Analysis for the Lifetime-Prediction and Failure Detection of Future Turbofan Components

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    Planetary gearbox and hydrodynamic journal bearings (HJB) are going to be integrated in future turbofan engines. This paper presents the results of applied methods to detect failures of these components. At first, failure detection requirements are derived by using system engineering techniques. In consideration of the identified failures theoretical assumptions are discussed and subsequently verified. Vibration and acoustic emission (AE) sensors seem promising to detect failures in an early stage. To prove the theoretical considerations experiments are carried out on test benches. Tooth flank damage of a planet gear in a planetary gearbox design is investigated. High demands are placed on the signal processing due to design-related amplitude modulation effects. Vibrations are measured using acceleration and AE sensors, which are mounted on the ring gear. The investigated failure type leads to excitation of non-stationary AE signals. It is proposed that the AE signals have a cyclostationary characteristic. Using cyclostationary-based processing techniques the signal’s hidden periodicities can be revealed. A separated analysis of each planet and evaluation of the envelope spectrum finally allows the detection of this failure type. Instead of roller bearings, HJB can be integrated in planet gears. The most essential damaging mechanism for HJB is wear as a result of mixed or boundary friction. These friction states are caused by conditions like Start/Stop Cycles, insufficient oil supply, overload or oil contamination. The accumulated intensity and duration of friction can be a measure of the remaining useful lifetime (RUL). To estimate the RUL friction has to be differentiated regarding the intensity. AE technology is a promising method to detect friction in HJB. Therefore, AE signals of the mentioned conditions are acquired. Due to rotating planet gears there is no possibility to place AE sensors directly on the surface of HJB. Finally suitable features for both components are extracted from the processed signals. Their separation efficiency with respect to the failure types is evaluated

    Ciliary and rhabdomeric photoreceptor-cell circuits form a spectral depth gauge in marine zooplankton.

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    This is the final version.Available from eLife Publications via the DOI in this record.All data generated or analysed during this study are included in the manuscript and supporting files.Source data files have been provided for Figures 1, 3 and 4 and Figure 2-figure supplement 2.Ciliary and rhabdomeric photoreceptor cells represent two main lines of photoreceptor-cell evolution in animals. The two cell types coexist in some animals, however how these cells functionally integrate is unknown. We used connectomics to map synaptic paths between ciliary and rhabdomeric photoreceptors in the planktonic larva of the annelid Platynereis and found that ciliary photoreceptors are presynaptic to the rhabdomeric circuit. The behaviors mediated by the ciliary and rhabdomeric cells also interact hierarchically. The ciliary photoreceptors are UV-sensitive and mediate downward swimming in non-directional UV light, a behavior absent in ciliary-opsin knockout larvae. UV avoidance overrides positive phototaxis mediated by the rhabdomeric eyes such that vertical swimming direction is determined by the ratio of blue/UV light. Since this ratio increases with depth, Platynereis larvae may use it as a depth gauge during vertical migration. Our results revealed a functional integration of ciliary and rhabdomeric photoreceptor cells in a zooplankton larva.The research was supported by a grant from the DFG - Deutsche Forschungsgemeinschaft (Reference no. JE 777/3–1). SY was supported by the National Institutes of Health (R01EY016400) and Emory University. KTR is supported by grants from the University of Vienna (research platform “Rhythms of Life”), the FWF (http://www.fwf.ac.at/en/) research project grant (#P28970), and the European Council under the European Community’s Seventh Framework Programme (FP7/2007-2013) ERC Grant Agreement 337011

    Potential of redcurrant protein-enriched fractions as emulsifier in oil–water-emulsions

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    Berry protein, due to its functional properties, was extracted from redcurrant pomace seeds (RPS) in aqueous media to utilise side-streams and increase the sustainability of food production. Emulsions containing 1% redcurrant seed protein and 10% rapeseed oil were prepared by high pressure homogenisation. The emulsions’ structure, droplet surface charge, stability, rheological behaviour, and colour were characterised by microscopy, dynamic laser light scattering, storage tests and bulk rheology including large amplitude oscillatory shear rheology, respectively. Protein extracts of RPS proved to stabilise oil–water-interfaces by formation of a protein film having non-linear flow characteristics. While at low to medium shear, the emulsions showed pseudoplastic behaviour, limited molecular mobility at the interface induced softening at high shear. The shear stability of the interfacial protein layer decreased with increasing pH, i.e. with increasing molecular charge. Emulsions were prone to flocculation and subsequent creaming, either because of low surface charge at low pH or due to bridging by proteins and polymerised polyphenol complexes under neutral conditions. Although redcurrants only occupy a small market share in the berry sector, they are of high importance for specialised local producers and results presented here might evince possibilities for the utilisation of other pomace types

    Expression dynamics and protein localization of rhabdomeric opsins in Platynereis larvae.

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    The larval stages of polychaete annelids are often responsive to light and can possess one to six eyes. The early trochophore larvae of the errant annelid Platynereis dumerilii have a single pair of ventral eyespots, whereas older nectochaete larvae have an additional two pairs of dorsal eyes that will develop into the adult eyes. Early Platynereis trochophores show robust positive phototaxis starting on the first day of development. Even though the mechanism of phototaxis in Platynereis early trochophore larvae is well understood, no photopigment (opsin) expression has yet been described in this stage. In late trochophore larvae, a rhabdomeric-type opsin, r-opsin1, expressed in both the eyespots and the adult eyes has already been reported. Here, we identify another Platynereis rhabdomeric opsin, r-opsin3, that is expressed in a single photoreceptor in the eyespots in early trochophores, suggesting that it mediates early larval phototaxis. We also show that r-opsin1 and r-opsin3 are expressed in adjacent photoreceptor cells in the eyespots in later stages, indicating that a second eyespot-photoreceptor differentiates in late trochophore larvae. Using serial transmission electron microscopy (TEM), we identified and reconstructed both photoreceptors and a pigment cell in the late larval eyespot. We also characterized opsin expression in the adult eyes and found that the two opsins co-express there in several photoreceptor cells. Using antibodies recognizing r-opsin1 and r-opsin3 proteins, we demonstrate that both opsins localize to the rhabdomere in all six eyes. In addition, we found that r-opsin1 mRNA is localized to, and translated in, the projections of the adult eyes. The specific changes we describe in opsin transcription and translation and in the cellular complement suggest that the six larval eyes undergo spectral and functional maturation during the early planktonic phase of the Platynereis life cycle
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