Influencia de los ácaros en la vehiculación de micosis cutáneas en micromamíferos

Tesi de Llicenciatura per a la obtenció del Grau de Farmàcia. Facultat de Farmàcia. Universitat de Barcelona. Director: Montserrat Portús Vinyeta. 1981

First cases of gynandromorphism in Phlebotomus perniciosus Newstead, 1911 (Diptera, Psychodidae, Phlebotominae)

Two gynandromorphic specimens of Phlebotomus perniciosus Newstead, 1911 are described and illustrated for the first time The specimens were collected in the Northeast of the Iberian Peninsula (Spain)

Is leishmaniosis spreading to northern areas of the Iberian Peninsula? The examples of Lleida (NE Spain) and Andorra

Podeu consultar el llibre complet a: http://hdl.handle.net/2445/46988The entomological and canine leishmaniosis surveys carried out in the northwest of Catalonia and in Andorra in the context of the European project Emerging Diseases in a changing European eNvironment (EDEN) are summarized. The aim of the study was to obtain data on the presence of leishmaniosis in these areas and the spatial distribution of their vectors

Is leishmaniasis endemic on the island of Minorca (Spain)?. A human visceral case after living 13 years in Minorca

Ecoepidemiologica studies performed in Minorca (Balearic Islands) seem to show that leishmaniasis is not endemic in this island, even in the presence of the vector. All cases of leishmaniasis in man or dog diagnosed in the island seem to come from other Spanish regions. A recent case of human visceral leishmaniasis in a man who had not left Minorca for 13 years calls into question this assumption or the time that Leishmania may persist asymptomatic in the host

Isoenzymatic characterization of Phlebotomus ariasi and P. perniciosus of canine leishmaniasis foci from Eastern Pyrenean regions and comparison with other populations from Europe.

An entomological survey was carried out in 2007 in two Pyrenean counties of Lleida province (northeastern Spain), where cases of autochthonous canine leishmaniasis have been recently reported. Phlebotomus ariasi and P. perniciosus, vectors of Leishmania infantum in the Mediterranean area, were captured. The aim of the present study was to compare these phlebotomine populations with others captured in known leishmaniasis foci in Europe. Populations of these species were studied by analysing the polymorphism of seven enzymatic systems (HK, PGI, PGM, MDH, 6PGD, FUM and ACO) and compared with other specimens from endemic regions of France, Italy, Malta, Portugal and Spain captured in other campaigns, and also with previously published results. Phlebotomus ariasi was more polymorphic than P. perniciosus. Only the ACO locus had diagnostic alleles, but some other alleles show high characteristic frequencies for each species. The neighbourjoining trees separated two population groups in both species. On the basis of the isoenzyme study results, sand fly populations of the Pyrenean region in Lleida province are closely related to those of other nearby leishmaniasis endemic regions in France and Spain

El diagnóstico serológico de la leishmaniosis canina en la comarca del Priorat (Tarragona)

Se investiga la presencia de anticuerpos anti-Leishmania mediante una técnica de «Dot-ELISA» en 1.328 muestras de sangre procedentes de 902 perros de la comarca del Priorat (Cataluña), importante foco de leishmaniosis canina. El umbral de positividad para la mencionada técnica (11800) se establece a partir de los datos obtenidos al realizar en paralelo cultivo y serología. Los resultados serológicos obtenidos permiten observar una tasa de prevalencia de la infección de 10,2%. Tan sólo el 49,8 % de los sueros estudiados son totalmente negativos. Al 40 % restante se le detecta anticuerpos anti-Leishmania a títulos inferiores al umbral establecido cuyo posible significado se discute.The presence of anti-Leishmania antibodies is studied in 1328 blood samples from 902 dogs from the Priorat region (Catalonia), an important focus of canine leúhmaniosú, by a Dot-ELISA technique. The cut-off (1/800)is established through the data obtained by serology and culture in parallel. The prevalence of seropositives observed was 10,2 %. Only 49,8 % of sera were completely negative. The remaining 40% had anti-Leishmania antibodies at titres below the cut- off, the possible significance of which is discused

Trois cas de gynandromorphisme chez <i>Sergentomyia minuta</i> (Diptera, Psychodidae) dans la Péninsule Ibérique

Three gynandromorphe specimens of Sergentomyia minuta have been identified among sandflies captured in North-East Spain. The simultaneous presence of more or less modified male genitalia and of fome female structures were the most conspicuous features encountred.</p

Application of microsatellite genotyping to the study of a restricted Leishmania infantum focus: different genotype compositions in isolates from dogs and sand flies

Leishmania infantum polymorphism was studied by DNA microsatellite analysis of 110 L. infantum stocks (94 from dogs, 15 from sand flies, and 1 from a human visceral case) from a rural leishmaniasis-endemic area (Priorat) in northeastern Spain. Three microsatellites of the eight present in three fragments (internal transcribed spacer, Lm4, and Lm2) of L. infantum nuclear DNA are polymorphic inside the focus, resulting in 17 genotypes. Isolates from dogs and sand flies had different allelic compositions and shared only four genotypes. Microsatellite analysis is useful for L. infantum genotyping and epidemiologic tracking. Its application with strains from dogs and vectors in an area endemic for leishmaniasis shows the heterogeneous distribution of L. infantum in hosts living in sympatric conditions