血中の2,3,4,7,8-五塩化ダイベンゾフラン(PeCDF)の個人の半減期：油症患者における臨床症状並びに検診結果との関係

BACKGROUND: In 1968, many people developed dioxin poisoning (Yusho) in Japan. Ingestion of 2,3,4,7,8-pentachlorodibenzofuran (2,3,4,7,8-PeCDF) was considered to be the cause of this poisoning. Although some patients had high concentrations of 2,3,4,7,8-PeCDF in their blood, individuals' half-lives of 2,3,4,7,8-PeCDF were long. OBJECTIVES: To evaluate the relationship between clinical and laboratory parameters and the individual half-life of 2,3,4,7,8-PeCDF in blood. METHODS: Clinical and laboratory data were collected during annual check-ups from 2001 to 2008. We enrolled 71 patients, who were measured more than 3 times, and who had 2,3,4,7,8-PeCDF concentrations in blood >50pgg(-1) lipid. The half-life of 2,3,4,7,8-PeCDF for each patient was estimated using linear regression. Moreover, relationships between clinical and laboratory parameters and individual half-life were investigated by linear regression. RESULTS: A shortened individual half-life for 2,3,4,7,8-PeCDF was significantly correlated with an increased red blood cell count, increased viscous secretions from the meibomian glands, existing black comedones, and severe cedar pollen allergy. CONCLUSIONS: Symptoms that accelerate excretion of lipids from the body, such as viscous secretions from the meibomian glands, may lead to a shorter half-life of 2,3,4,7,8-PeCDF. Red blood cells are related to the half-life of 2,3,4,7,8-PeCDF. However, further studies are required to investigate the excretory mechanism of 2,3,4,7,8-PeCDF.博士（医学）・乙1325号・平成26年3月17

Role of the Aromatic Ring of Tyr43 in Tetraheme Cytochrome c(3) from Desulfovibrio vulgaris Miyazaki F

Tyrosine 43 is positioned parallel to the fifth heme axial ligand, His34, of heme 1 in the tetraheme cytochrome c(3). The replacement of tyrosine with leucine increased the redox potential of heme 1 by 44 and 35 mV at the first and last reduction steps, respectively; its effects on the other hemes are small. In contrast, the Y43F mutation hardly changed the potentials. It shows that the aromatic ring at this position contributes to lowering the redox potential of heme 1 locally, although this cannot be the major contribution to the extremely low redox potentials of cytochrome c(3). Furthermore, temperature-dependent line-width broadening in partially reduced samples established that the aromatic ring at position 43 participates in the control of the kinetics of intramolecular electron transfer. The rate of reduction of Y43L cytochrome c(3) by 5-deazariboflavin semiquinone under partially reduced conditions was significantly different from that of the wild type in the last stage of the reduction, supporting the involvement of Tyr43 in regulation of reduction kinetics. The mutation of Y43L, however, did not induce a significant change in the crystal structure

Prevalence of Atopic Dermatitis and Serum IgE of Yusho Patients Born before 1967

特集号 油症とPCB及びダイキシン関連化合物 研究報告 第23集 責任編集者 古江増隆The Twenty-third Reports of the Study on Yusho―PCBs and Dioxin-Related Compounds―Guest Editor Furue MasutakaDioxins may have an impact on the human immunological system, which would increase the risk to develop allergic diseases, such as atopic dermatitis. In order to determine the lifetime prevalence of atopic dermatitis in Yusho patients, a questionnaire-based survey was conducted in 2008. One thousand and seventy-one out of 1430 certified yusho patients who were born before Yusho accident answered the questionnaires, and the prevalence of atopic dermatitis in Yusho patients was 5.5%. We also measured serum IgE in 515 Yusho patients who attended annual medical check-ups from 2007 to 2009 and in 172 control subjects. Serum levels of IgE in Yusho patients were 250.7 ± 663.4 IU/ml, whereas those in control subjects were 265.0 ± 602.0 IU/ml. There was no significant difference in serum levels of IgE between Yusho patients and control subjects. In addition, no significant correlation was observed between serum levels of IgE and blood levels of dioxins in Yusho patients.油症発生から40 年が経過し，発症早期に認められた典型的な皮膚科所見，眼科所見を有する患者は減少しているが，発癌性や次世代への影響など慢性毒性への懸念は依然強い．ダイオキシン類の慢性毒性の一つとして，免疫系への影響があげられ，近年のアレルギー性疾患の増加とダイオキシン類による環境汚染との関連が議論されることがある．本研究では，経口的に高濃度のダイオキシン類に暴露された油症患者における，アトピー性皮膚炎有病率および血清IgE 値について調査し，血中ダイオキシン類濃度との相関について検討した

Chemokine Profile of Yusho Patients

特集号 油症とPCB及びダイキシン関連化合物 研究報告 第23集 責任編集者 古江増隆The Twenty-third Reports of the Study on Yusho―PCBs and Dioxin-Related Compounds―Guest Editor Furue MasutakaDioxins may have an impact on the human immunological system, which would increase the risk to develop allergic diseases, such as atopic dermatitis. In the present study, we measured serum levels of Th1- and Th2-favored chemokines in 233 Yusho patients who attended annual medical check-ups from 2006 to 2009 and in 97 control subjects. Serum levels of CCL5, CCL17, and CCL27 in Yusho patients were significantly lower than those in control subjects. In addition, serum levels of some chemokines have weak correlations with blood levels of dioxins in either Yusho patients or control subjects.ダイオキシン類の慢性毒性の一つとして免疫系 への影響があげられ，近年のアレルギー性疾患の 増加とダイオキシン類による環境汚染との関連が議論されることがある．本研究では，現在でも高い血中ダイオキシン類濃度を有する油症患者を対象に，Th1，Th2 環境で高値を示すケモカインを中心に各種血清ケモカイン濃度を測定し，ダイオキシン類の影響について検討した

油症認定患者におけるアトピー性皮膚炎有病率と血清IgE値に関する研究第2報

Dioxins may have an impact on the human immunological system, which would increase the risk to develop allergic diseases, such as atopic dermatitis. In order to determine the lifetime prevalence of atopic dermatitis in Yusho patients, a questionnaire-based survey was conducted in 2008. One thousand and seventy-one out of 1430 certified yusho patients who were born before Yusho accident answered the questionnaires, and the prevalence of atopic dermatitis in Yusho patients was 5.5%. We also measured serum IgE in 515 Yusho patients who attended annual medical check-ups from 2007 to 2009 and in 172 control subjects. Serum levels of IgE in Yusho patients were 250.7 ± 663.4 IU/ml, whereas those in control subjects were 265.0 ± 602.0 IU/ml. There was no significant difference in serum levels of IgE between Yusho patients and control subjects. In addition, no significant correlation was observed between serum levels of IgE and blood levels of dioxins in Yusho patients.油症発生から40 年が経過し，発症早期に認められた典型的な皮膚科所見，眼科所見を有する患者は減少しているが，発癌性や次世代への影響など慢性毒性への懸念は依然強い．ダイオキシン類の慢性毒性の一つとして，免疫系への影響があげられ，近年のアレルギー性疾患の増加とダイオキシン類による環境汚染との関連が議論されることがある．本研究では，経口的に高濃度のダイオキシン類に暴露された油症患者における，アトピー性皮膚炎有病率および血清IgE 値について調査し，血中ダイオキシン類濃度との相関について検討した．特集号 油症とPCB及びダイキシン関連化合物 研究報告 第23集 責任編集者 古江増隆The Twenty-third Reports of the Study on Yusho―PCBs and Dioxin-Related Compounds―Guest Editor Furue Masutak

油症認定患者における血清ケモカイン濃度に関する研究

Dioxins may have an impact on the human immunological system, which would increase the risk to develop allergic diseases, such as atopic dermatitis. In the present study, we measured serum levels of Th1- and Th2-favored chemokines in 233 Yusho patients who attended annual medical check-ups from 2006 to 2009 and in 97 control subjects. Serum levels of CCL5, CCL17, and CCL27 in Yusho patients were significantly lower than those in control subjects. In addition, serum levels of some chemokines have weak correlations with blood levels of dioxins in either Yusho patients or control subjects.ダイオキシン類の慢性毒性の一つとして免疫系 への影響があげられ，近年のアレルギー性疾患の 増加とダイオキシン類による環境汚染との関連が議論されることがある．本研究では，現在でも高い血中ダイオキシン類濃度を有する油症患者を対象に，Th1，Th2 環境で高値を示すケモカインを中心に各種血清ケモカイン濃度を測定し，ダイオキシン類の影響について検討した．特集号 油症とPCB及びダイキシン関連化合物 研究報告 第23集 責任編集者 古江増隆The Twenty-third Reports of the Study on Yusho―PCBs and Dioxin-Related Compounds―Guest Editor Furue Masutak

Development of an efficient one-step real-time reverse transcription polymerase chain reaction method for severe acute respiratory syndrome-coronavirus-2 detection.

The general methods to detect the RNA of severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) in clinical diagnostic testing involve reverse transcriptases and thermostable DNA polymerases. In this study, we compared the detection of SARS-CoV-2 by a one-step real-time RT-PCR method using a heat-resistant reverse transcriptase variant MM4 from Moloney murine leukemia virus, two thermostable DNA polymerase variants with reverse transcriptase activity from Thermotoga petrophila K4 and Thermococcus kodakarensis KOD1, or a wild-type DNA polymerase from Thermus thermophilus M1. The highest performance was achieved by combining MM4 with the thermostable DNA polymerase from T. thermophilus M1. These enzymes efficiently amplified specific RNA using uracil-DNA glycosylase (UNG) to remove contamination and human RNase P RNA amplification as an internal control. The standard curve was obtained from 5 to 105 copies of synthetic RNA. The one-step real-time RT-PCR method's sensitivity and specificity were 99.44% and 100%, respectively (n = 213), compared to those of a commercially available diagnostic kit. Therefore, our method will be useful for the accurate detection and quantification of SARS-CoV-2