Analysis of the overhead throwing motion in softball and baseball

<p>Ref indicates the nucleotide sequences of the ORF 73 gene of the OvHV-2 reference genomes obtained from the GenBank. Variable regions are highlighted in rectangles (▯), and groups of genotypes (G) are illustrated on the right side of the align sequences.</p

Summary of genotypes of different genes investigated for characterization of South African OvHV-2 strains.

<p>Summary of genotypes of different genes investigated for characterization of South African OvHV-2 strains.</p

The phylogenetic trees of the O RF 73 nucleotide (A & B) and amino acid (C & D) sequences of OvHV-2 strains and published closely related reference sequences obtained from GenBank constructed using Neighbor-Joining (A & C) and Maximum Likelihood (B & D) methods.

<p>The unrooted condensed trees were constructed using Neighbor-Joining (A & C) and Maximum Likelihood (B & D) methods, showing branching patterns of each clade. Groups 1 and 2 are illustrated and abbreviated as clade 1 (C1) and 2 (C2), respectively, with supporting bootstrap values at each clade nodes.</p

The list of bovine blood samples, obtained from different geographic areas in South Africa, used for molecular characterization of OvHV-2.

<p>The list of bovine blood samples, obtained from different geographic areas in South Africa, used for molecular characterization of OvHV-2.</p

The phylogenetic trees of the ORF 27 nucleotide (A & B) and amino acid (C & D) sequences of OvHV-2 strains and their closely related reference sequences obtained from GenBank constructed using Neighbor-Joining (A & C) and Maximum Likelihood (B & D) methods.

<p>The unrooted condensed trees were constructed using Neighbor-Joining (A & C) and Maximum Likelihood (B & D) methods, showing branching patterns of each clade. Groups 1 and 2 are illustrated and abbreviated as clade 1 (C1) and 2 (C2), respectively, with supporting bootstrap values at each clade nodes.</p

Oligonucleotide primer sequences used for specific amplification of Ov 8 ex2, Ov 7, ORF 27 and ORF 73 genes, and indicating positions of each gene on the OvHV-2 reference genome sequences.

<p>Oligonucleotide primer sequences used for specific amplification of Ov 8 ex2, Ov 7, ORF 27 and ORF 73 genes, and indicating positions of each gene on the OvHV-2 reference genome sequences.</p

Multiple sequence alignment of nucleotide sequences of the Ov 7 gene from SA OvHV-2 strains and reference sequences derived from OvHV-2 genome sequences (Accession numbers: NC-007646.1, AY839756.1, DQ198083.1), showing three genotypes of Ov 7 gene.

<p>Ref on the alignment indicates the nucleotide sequences of the Ov 7 gene of the OvHV-2 reference genomes obtained from the GenBank. Variable regions are highlighted in rectangles (▯) and group of genotypes (G) are illustrated on the right side of the align sequences.</p

Multiple sequence alignment of amino acid sequences of the ORF 73 gene from SA OvHV-2 strains and reference sequences derived from OvHV-2 genome sequences (Accession numbers: NC-007646.1, AY839756.1, DQ198083.1) analysed with ExPASy translate tool.

<p>Ref indicates the amino acid sequences of the ORF 73 gene of the OvHV-2 reference genomes obtained from the GenBank. Variable regions are highlighted in rectangles (▯), and groups of amino acid sequences (G) are illustrated on the right side of the align sequences</p

The phylogenetic trees of the Ov 7 nucleotide (A & B) and amino acid (C & D) sequences of OvHV-2 strains and their closely related reference sequences obtained from GenBank.

<p>The unrooted condensed trees were constructed using Neighbor-Joining (A & C) and Maximum Likelihood (B & D) methods, showing branching patterns of each clade. Groups 1, 2 and 3 are illustrated and abbreviated as clade 1 (C1), 2 (C2) and 3 C3), respectively, with supporting bootstrap values at each clade nodes.</p

A representation of PCR products obtained from amplification of complete glycoprotein genes, Ov 7 (495 bp), Ov 8 ex2 (253 bp), ORF 27 (999 bp) and the tegument gene, ORF 73 (1649 bp) of OvHV-2.

<p>A representation of PCR products obtained from amplification of complete glycoprotein genes, Ov 7 (495 bp), Ov 8 ex2 (253 bp), ORF 27 (999 bp) and the tegument gene, ORF 73 (1649 bp) of OvHV-2.</p