Endocytosis of the ASGP receptor H1 is reduced by mutation of tyrosine-5 but still occurs via coated pits

The clustering of plasma membrane receptors in clathrin-coated pits depends on determinants within their cytoplasmic domains. In several cases, individual tyrosine residues were shown to be necessary for rapid internalization. We have mutated the single tyrosine at position 5 in the cytoplasmic domain of the major subunit H1 of the asialoglycoprotein receptor to alanine. Expressed in fibroblasts cells, the mutant protein was accumulated in the plasma membrane, and its rate of internalization was reduced by a factor of four. The residual rate of endocytosis, however, was still significantly higher than that of resident plasma membrane proteins. Upon acidification of the cytoplasm, which specifically inhibits the formation of clathrin-coated vesicles but not uptake of the fluid phase marker Lucifer yellow, residual endocytosis was blocked. By immunoelectron microscopy mutant H1 could be directly demonstrated in coated pits. The fraction of wild-type and mutant H1 present in coated pits as determined by immunogold localization correlated well with the respective rates of internalization. Thus, mutation of tyrosine-5 only partially inactivates recognition of H1 for incorporation into coated pits

Spin Effects in a Quantum Ring

Recent experiments are reviewed that explore the spin states of a ring-shaped many-electron quantum dot. Coulomb-blockade spectroscopy is used to access the spin degree of freedom. The Zeeman effect observed for states with successive electron number allows to select possible sequences of spin ground states of the ring. Spin-paired orbital levels can be identified by probing their response to magnetic fields normal to the plane of the ring and electric fields caused by suitable gate voltages. This narrows down the choice of ground-state spin sequences. A gate-controlled singlet--triplet transition is identified and the size of the exchange interaction matrix element is determined.Comment: 13 pages, 3 figures, Proceedings of the QD2004 conference in Banf

Issues in economics: what is the cost of deflation?

A steady decline in the core rate of inflation over the past few years has prompted the question: What are the economic consequences when the inflation rate drops below zero?Deflation (Finance)

Transmission Phase Through Two Quantum Dots Embedded in a Four-Terminal Quantum Ring

We use the Aharonov-Bohm effect in a four-terminal ring based on a Ga[Al]As heterostructure for the measurement of the relative transmission phase. In each of the two interfering paths we induce a quantum dot. The number of electrons in the two dots can be controlled independently. The transmission phase is measured as electrons are added to or taken away from the individual quantum dots.Comment: 3 pages, 4 figure

Microwave shielding of transparent and conducting single-walled carbon nanotube films

The authors measured the transport properties of single-walled carbon nanotube (SWCNT) films in the microwave frequency range from 10 MHz to 30 GHz by using the Corbino reflection technique from temperatures of 20-400 K. Based on the real and imaginary parts of the microwave conductivity, they calculated the shielding effectiveness for various film thicknesses. Shielding effectiveness of 43 dB at 10 MHz and 28 dB at 10 GHz are found for films with 90% optical transmittance, which suggests that SWCNT films are promising as a type of transparent microwave shielding material. By combining their data with those from the literature, the conductivity of SWCNT films was established in a broad frequency range from dc to visible.Comment: 4 pages, 4 figure

Kondo Effect in a Many-Electron Quantum Ring

The Kondo effect is investigated in a many-electron quantum ring as a function of magnetic field. For fields applied perpendicular to the plane of the ring a modulation of the Kondo effect with the Aharonov-Bohm period is observed. This effect is discussed in terms of the energy spectrum of the ring and the parametrically changing tunnel coupling. In addition, we use gate voltages to modify the ground-state spin of the ring. The observed splitting of the Kondo-related zero-bias anomaly in this configuration is tuned with an in-plane magnetic field.Comment: 4 pages, 4 figure

Singlet-Triplet Transition Tuned by Asymmetric Gate Voltages in a Quantum Ring

Wavefunction and interaction effects in the addition spectrum of a Coulomb blockaded many electron quantum ring are investigated as a function of asymmetrically applied gate voltages and magnetic field. Hartree and exchange contributions to the interaction are quantitatively evaluated at a crossing between states extended around the ring and states which are more localized in one arm of the ring. A gate tunable singlet-triplet transition of the two uppermost levels of this many electron ring is identified at zero magnetic field.Comment: 4 page