Ultra-large sheet formation by 1D to 2D hierarchical self-assembly of a “rod–coil” graft copolymer with a polyphenylene backbone

This communication reports a unique ultra-large sheet formation through hierarchical self-assembly of a rod–coil graft copolymer containing a rigid polyphenylene backbone and flexible poly(ethylene oxide) (PEO) side chains. The hierarchical self-assembly process involved a distinctive morphological transition of 1D helical to 2D superstructures. The graft copolymer offers a new chance for the challenging bottom-up fabrication of ultra-large self-assembled nanosheets in solution, as well as a novel system for fundamental studies on 2D self-assembly of polymers

Parameter optimization analysis of rotary electromagnetic vibration energy harvester for performance enhancement under free vibration

Summary: In this paper, three new important aspects of rotary electromagnetic vibration energy harvesting technology (RE-VEH) are concerned and investigated: (i) vibro-electric coupling mechanism of the RE-VEH system is studied through theoretical modeling; (ii) quantitative analysis of system parameters based on numerical simulation method is carried out for the optimal design of RE-VEH; and (iii) dynamic power output performance of the RE-VEH system in free vibration is discussed. The parameter adjusting methods of the RE-VEH system in free vibration mode are obtained through theoretical analysis and numerical simulation. The experimental results show that the power output performance of RE-VEH in free vibration mode matches the numerical simulation results. The simulation and experimental results show that the maximum voltage output and power output of the RE-VEH with different structure parameters under free vibration can be up to the level of 100∼101 V/watt. The above results indicate that RE-VEH in a free vibration environment has significant energy output performance

Ischemia-Challenged human umbilical vascular endothelial cells: Proteomics data

[Human umbilical vascular endothelial cells (HUVECs) underwent ischemia, ischemia/reperfusion and normal control (sham) treatment and marked as group I, IR and NC, respectively, were detected by quantitative proteomics and bioinformatics analyses. Proteins in Beclin-1/LC3-II-dependent canonical macroautophagy pathway were verified in details. The significantly upregulated proteins encoded by autophagy-related genes (ATGs) included ATG2A, ATG3, ATG4B, ATG5, ATG7, ATG9A, ATG12, ATG16 and ATG101. The significantly enhanced lysosomal proteins comprised Cathepsin B, Cathepsin D, lysosome-associated membrane protein 1 (LAMP1) and LAMP2. However, the differentially expressed proteins excluded Beclin-1, microtubule-associated protein light chain 3 (LC3)-I and LC3-II. Western blot analyses verified that the protein expressions of Beclin-1, LC3-I and LC3-II were neither upregulated nor downregulated in ischemia-challenged HUVECs.

Three-Dimensional Structure Analysis and Droplet Spectrum Characteristics of Southwest Vortex Precipitation System Based on GPM-DPR

This study is the first in the region to use Global Precipitation Mission Dual-Frequency Precipitation Radar (GPM-DPR) and Fengyun-2G (FY-2G) observations to qualitatively and quantitatively study the Southwest Vortex evolution characteristics during the flood season from 2019 to 2021. Furthermore, vertical characteristics of the two main precipitation types in the Southwest Vortex, stratiform and convective, were statistically analyzed at different life stages, including horizontal and vertical distribution of precipitation particles, droplet spectrum characteristics, and vertically layered precipitation contribution. The results showed that: (1) The typical convective precipitation (CP) in the developing and mature stages has strong reflectivity distribution centers in the upper and lower layers, showing characteristics related to terrain. Additionally, the high-level hydrometeor particles are mainly solid precipitation particles, and particles in the lower layers collide and coalesce in the violent vertical motion of the airflow. (2) For the three stages of CP, the reflectivity below melting layer (ML) first showed a rapid weakening trend toward the surface and then remained unchanged, significantly changing its vertical structure. The main rainfall type of the Southwest Vortex system was stratiform precipitation (SP) in the three stages. (3) In the two types of cloud precipitation, the developing stage is generally composed of large and sparse precipitation particles, the mature stage of large and dense precipitation particles, and the dissipating stage of small and sparse precipitation particles. The findings of this study reveal the three-dimensional refined structure and vertical variation characteristics of different life stages of the Southwest Vortex precipitation cloud system and provide important tools and references for improving the accuracy of numerical models and the forecast level of short-term heavy precipitation under complex terrain

Pro-Inflammatory Cytokine TNF-α Attenuates BMP9-Induced Osteo/ Odontoblastic Differentiation of the Stem Cells of Dental Apical Papilla (SCAPs)

Background/Aims: Periapical periodontitis is a common oral disease caused by bacterial invasion of the tooth pulp, which usually leads to local release of pro-inflammatory cytokines and osteolytic lesion. This study is intended to examine the effect of TNF-α on BMP9-induced osteogenic differentiation of the stem cells of dental apical papilla (SCAPs). Methods: Rat model of periapical periodontitis was established. TNF-α expression was assessed. Osteogenic markers and ectopic bone formation in iSCAPs were analyzed upon BMP9 and TNF-α treatment. Results: Periapical periodontitis was successfully established in rat immature permanent teeth with periapical lesions, in which TNF-α was shown to release during the inflammatory phase. BMP9-induced alkaline phosphatase activity, the expression of osteocalcin and osteopontin, and matrix mineralization in iSCAPs were inhibited by TNF-α in a dose-dependent fashion, although increased AdBMP9 partially overcame TNF-α inhibition. Furthermore, high concentration of TNF-α effectively inhibited BMP9-induced ectopic bone formation in vivo. Conclusion: TNF-α plays an important role in periapical bone defect during the inflammatory phase and inhibits BMP9-induced osteoblastic differentiation of iSCAPs, which can be partially reversed by high levels of BMP9. Therefore, BMP9 may be further explored as a potent osteogenic factor to improve osteo/odontogenic differentiation in tooth regeneration in chronic inflammation conditions

P. aeruginosa lipopolysaccharide-induced MUC5AC and CLCA3 expression is partly through Duox1 in vitro and in vivo.

We have previously found that reactive oxygen species (ROS) are involved in Pseudomonas aeruginosa lipopolysaccharide (PA-LPS) induced MUC5AC in airway epithelial cells. Dual oxidase1 (Duox1), a member of NADPH oxidase(Nox), is known to be responsible for ROS production in respiratory tract epithelial cells. Our aim was to clarify whether Duox1 was also involved in the PA-LPS-induced MUC5AC and calcium dependent chloride channel 3(Clca3), another recognized marker of goblet cell hyperplasia and mucus hyper-production.PA-LPS-induced Duox1 mRNA levels were examined in A549 cells, primary mouse tracheal epithelial cells (mTECS) and lung tissues of mice. Nox inhibitors diphenyleneiodonium chloride (DPI) and Duox1 siRNA were used to investigate whether Duox1 is involved in PA-LPS-induced MUC5AC and Clca3 expression both in vitro and in vivo.Duox1 is induced by PA-LPS in A549 cells, primary mTECs and lung tissues of mice. DPI significantly inhibited PA-LPS-induced up-regulation of Duox1, Muc5ac and Clca3 in primary mouse trachea epithelial cells and lung tissues of mice. Knockdown of Duox1 markedly inhibited PA-LPS-induced MUC5AC expression via a ROS-TGF-α cascade in A549 cells. Furthermore, DPI significantly inhibited PA-LPS-induced increases in inflammatory cells accumulated in mouse lungs.We demonstrate for the first time that PA-LPS-induced MUC5AC and Clca3 expression is partly through Duox1, and provide supportive evidence for Duox1 as a potential target in treatments of mucin over-production diseases

Genome-Wide Identification and Functional Characterization of Stress Related Glyoxalase Genes in <i>Brassica napus</i> L.

Rapeseed (Brassica napus L.) is not only one of the most important oil crops in the world, but it is also an important vegetable crop with a high value nutrients and metabolites. However, rapeseed is often severely damaged by adverse stresses, such as low temperature, pathogen infection and so on. Glyoxalase I (GLYI) and glyoxalase II (GLYII) are two enzymes responsible for the detoxification of a cytotoxic metabolite methylglyoxal (MG) into the nontoxic S-D-lactoylglutathione, which plays crucial roles in stress tolerance in plants. Considering the important roles of glyoxalases, the GLY gene families have been analyzed in higher plans, such as rice, soybean and Chinese cabbage; however, little is known about the presence, distribution, localizations and expression of glyoxalase genes in rapeseed, a young allotetraploid. In this study, a total of 35 BnaGLYI and 30 BnaGLYII genes were identified in the B. napus genome and were clustered into six and eight subfamilies, respectively. The classification, chromosomal distribution, gene structure and conserved motif were identified or predicted. BnaGLYI and BnaGLYII proteins were mainly localized in chloroplast and cytoplasm. By using publicly available RNA-seq data and a quantitative real-time PCR analysis (qRT-PCR), the expression profiling of these genes of different tissues was demonstrated in different developmental stages as well as under stresses. The results indicated that their expression profiles varied among different tissues. Some members are highly expressed in specific tissues, BnaGLYI11 and BnaGLYI27 expressed in flowers and germinating seed. At the same time, the two genes were significantly up-regulated under heat, cold and freezing stresses. Notably, a number of BnaGLY genes showed responses to Plasmodiophora brassicae infection. Overexpression of BnGLYI11 gene in Arabidopsis thaliana seedlings confirmed that this gene conferred freezing tolerance. This study provides insight of the BnaGLYI and BnaGLYII gene families in allotetraploid B. napus and their roles in stress resistance, and important information and gene resources for developing stress resistant vegetable and rapeseed oil

Whole-Genome Comparison Reveals Structural Variations behind Heading Leaf Trait in <i>Brassica oleracea</i>

Brassica oleracea displays remarkable morphological variations. It intrigued researchers to study the underlying cause of the enormous diversification of this organism. However, genomic variations in complex heading traits are less known in B. oleracea. Herein, we performed a comparative population genomics analysis to explore structural variations (SVs) responsible for heading trait formation in B. oleracea. Synteny analysis showed that chromosomes C1 and C2 of B. oleracea (CC) shared strong collinearity with A01 and A02 of B. rapa (AA), respectively. Two historical events, whole genome triplication (WGT) of Brassica species and differentiation time between AA and CC genomes, were observed clearly by phylogenetic and Ks analysis. By comparing heading and non-heading populations of B. oleracea genomes, we found extensive SVs during the diversification of the B. oleracea genome. We identified 1205 SVs that have an impact on 545 genes and might be associated with the heading trait of cabbage. Overlapping the genes affected by SVs and the differentially expressed genes identified by RNA-seq analysis, we identified six vital candidate genes that may be related to heading trait formation in cabbage. Further, qRT-PCR experiments also verified that six genes were differentially expressed between heading leaves and non-heading leaves, respectively. Collectively, we used available genomes to conduct a comparison population genome analysis and identify candidate genes for the heading trait of cabbage, which provides insight into the underlying reason for heading trait formation in B. oleracea